Sex Differences in the Serum Proteomic Profile During Acute Low Back Pain—A Preliminary Study of the Relationship to Future Low Back Pain

Jenkins, Luke C.; Chang, Wei-Ju; Humburg, Peter; Wasinger, Valerie C.; Stone, Laura S.; Dorsey, Susan G.; Renn, Cynthia; Starkweather, Angela; Schabrun, Siobhan M.

doi:10.1016/j.jpain.2023.11.009

Cited by 2 publications

(1 citation statement)

References 96 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…LBP is the most common chronic pain condition, is the leading cause of disability globally, and is increasing in prevalence more rapidly than any other chronic pain condition (2)(3)(4). This burden and the prevalence and incidence of LBP is higher in women than men (2,4,5). Although injury or accumulated damage to the discovertebral complex, annulus fibrosis, internal intervertebral disc structures, facet joints and/or sacroiliac joint can cause acute episodes, the development and maintenance of persistent LBP generally involves complex mechanisms including interactions between the immune and nervous systems.…”

Section: Introductionmentioning

confidence: 99%

Characterization of pain-related behaviors in a rat model of acute-to-chronic low back pain: single vs. multi-level disc injury

Barbe,

Chen,

Loomis

et al. 2024

Front. Pain Res.

Self Cite

View full text Add to dashboard Cite

IntroductionLow back pain is the most common type of chronic pain. We examined pain-related behaviors across 18 weeks in rats that received injury to one or two lumbar intervertebral discs (IVD) to determine if multi-level disc injuries enhance/prolong pain.MethodsTwenty-three Sprague-Dawley adult female rats were used: 8 received disc puncture (DP) of one lumbar IVD (L5/6, DP-1); 8 received DP of two lumbar IVDs (L4/5 & L5/6, DP-2); 8 underwent sham surgery.ResultsDP-2 rats showed local (low back) sensitivity to pressure at 6- and 12-weeks post-injury, and remote sensitivity to pressure (upper thighs) at 12- and 18-weeks and touch (hind paws) at 6, 12 and 18-weeks. DP-1 rats showed local and remote pressure sensitivity at 12-weeks only (and no tactile sensitivity), relative to Sham DP rats. Both DP groups showed reduced distance traveled during gait testing over multiple weeks, compared to pre-injury; only DP-2 rats showed reduced distance relative to Sham DP rats at 12-weeks. DP-2 rats displayed reduced positive interactions with a novel adult female rat at 3-weeks and hesitation and freezing during gait assays from 6-weeks onwards. At study end (18-weeks), radiological and histological analyses revealed reduced disc height and degeneration of punctured IVDs. Serum BDNF and TNFα levels were higher at 18-weeks in DP-2 rats, relative to Sham DP rats, and levels correlated positively with remote sensitivity in hind paws (tactile) and thighs (pressure).DiscussionThus, multi-level disc injuries resulted in earlier, prolonged and greater discomfort locally and remotely, than single-level disc injury. BDNF and TNFα may have contributing roles.

show abstract

Section: Introductionmentioning

confidence: 99%

Characterization of pain-related behaviors in a rat model of acute-to-chronic low back pain: single vs. multi-level disc injury

Barbe,

Chen,

Loomis

et al. 2024

Front. Pain Res.

Self Cite

View full text Add to dashboard Cite

show abstract

Neuroimmune mechanisms of a mouse model of chronic back pain

Goins,

Zurek,

Holguin

et al. 2024

Preprint

View full text Add to dashboard Cite

Chronic back pain (CBP) is the leading cause of disability affecting 1 in 10 people worldwide. Symptoms are marked by persistent lower back pain, reduced mobility, and heightened cold sensitivity. Here, we utilize a mouse model of CBP induced by injecting urokinase-type plasminogen activator (uPA), a proinflammatory agent in the fibrinolytic pathway, between the L2/L3 lumbar vertebrae. We identified neuroimmune interactions contributing to uPA-induced CBP (henceforth, uPA-CBP) in mouse dorsal root ganglia (DRG), where nociceptive neurons reside. Flow cytometric data reveal that uPA-CBP increases CD45+CD11b+ cells in the DRG, a population characteristically implicated in other chronic pain models1. Blocking colony stimulating factor 1 receptor (CSF1R) signaling using PLX5622 partially reduced pain, suggesting CD45+CD11b+ macrophage involvement. Whole-cell patch-clamp electrophysiology data indicated DRG neuron hyperexcitability in CBP mice compared to controls. RNA sequencing revealed upregulation of pain- and inflammation-related genes involved in leukocyte migration. Together, these findings underscore the importance of the DRG neuroimmune axis in mediating chronic back pain.HighlightsuPA-CBP induces gait changes, mechanical and thermal sensitivity compared to shamsuPA-CBP mice show increased CD45+CD11b+ cells in DRG compared to shamsuPA-CBP mice show neuronal excitability in DRG neurons compared to shamsPain behaviors are alleviated by pharmacologically blocking CSF1R signalingDysregulation of inflammation- and ion channel-related genes in uPA-CBP DRG

show abstract

Sex Differences in the Serum Proteomic Profile During Acute Low Back Pain—A Preliminary Study of the Relationship to Future Low Back Pain

Cited by 2 publications

References 96 publications

Characterization of pain-related behaviors in a rat model of acute-to-chronic low back pain: single vs. multi-level disc injury

Characterization of pain-related behaviors in a rat model of acute-to-chronic low back pain: single vs. multi-level disc injury

Neuroimmune mechanisms of a mouse model of chronic back pain

Contact Info

Product

Resources

About