2022
DOI: 10.1007/s11064-022-03757-z
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Sex Dimorphic Glucose Transporter-2 Regulation of Hypothalamic Astrocyte Glucose and Energy Sensor Expression and Glycogen Metabolism

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Cited by 8 publications
(15 citation statements)
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“…Incorporation of a plan to investigate potential sex differences in GLUT2 regulation of hypothalamic astrocyte MAPK family protein expression and phosphorylation aligns with the current U.S. National Institutes of Health policy emphasis on consideration of sex as a critical biological variable. This focus is supported by reports that the MAPK profile is sex-dimorphic in several organs [22][23][24][25][26] and is sensitive to estradiol [27], and by recent findings that GLUT2 imposes sex-specific control of astrocyte glucose and glycogen metabolism [15]. The work performed herein used siRNA gene silencing tools along with immunoblotting to determine if and how GLUT2 gene knockdown may affect total versus phosphorylated protein expression profiles for p44/21 MAPK (ERK1/2), p38 MAPK, and SAPK/JNK molecular-weight isoforms in glucose-supplied versus glucose-deprived hypothalamic astrocytes from each sex.…”
Section: Introductionmentioning
confidence: 79%
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“…Incorporation of a plan to investigate potential sex differences in GLUT2 regulation of hypothalamic astrocyte MAPK family protein expression and phosphorylation aligns with the current U.S. National Institutes of Health policy emphasis on consideration of sex as a critical biological variable. This focus is supported by reports that the MAPK profile is sex-dimorphic in several organs [22][23][24][25][26] and is sensitive to estradiol [27], and by recent findings that GLUT2 imposes sex-specific control of astrocyte glucose and glycogen metabolism [15]. The work performed herein used siRNA gene silencing tools along with immunoblotting to determine if and how GLUT2 gene knockdown may affect total versus phosphorylated protein expression profiles for p44/21 MAPK (ERK1/2), p38 MAPK, and SAPK/JNK molecular-weight isoforms in glucose-supplied versus glucose-deprived hypothalamic astrocytes from each sex.…”
Section: Introductionmentioning
confidence: 79%
“…no. B-002000-UB-100; Horizon Discovery), using reported methods [15]. This knockdown paradigm results in approximate 50% reductions in gene product expression across all treatment groups [15].…”
Section: Methodsmentioning
confidence: 99%
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“…GPER imposes bidirectional control of VMNvl glycogen amassment, for example, inhibitory during normoglycemia versus stimulatory upon decline in glycemic profiles, inferring that direction is subject to energy status. VMNvl astrocytes likely affect this switch in response to intrinsic energy monitoring as these glial express glucose and energy sensors [51]. Evidence for GPER inhibition of VMNvl astrocyte GPbb and GPmm protein expression during IIH infers that an absolute reduction in one or both variants may contribute to increased glycogen accrual in that location.…”
Section: Discussionmentioning
confidence: 99%