Sex Pheromone of the Tsetse Fly: Isolation, Identification, and Synthesis of Contact Aphrodisiacs

Carlson, D. A.; Langley, P. A.; Huyton, P. M.

doi:10.1126/science.675256

Cited by 144 publications

(76 citation statements)

References 12 publications

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“…Cuticular hydrocarbons long have been proposed to play a role in courting behavior and mating (Carlson et al 1978;Markow and Toolson 1990). We observed a significant upregulation of desaturase-2 (desat2) in D. mojavensis females following mating.…”

Section: Discussionmentioning

confidence: 73%

Transcriptional Regulation of Metabolism Associated With the Increased Desiccation Resistance of the Cactophilic Drosophila mojavensis

Matzkin

Markow²

2009

Genetics

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In Drosophila, adaptation to xeric environments presents many challenges, greatest among them the maintenance of water balance. Drosophila mojavensis, a cactophilic species from the deserts of North America, is one of the most desiccation resistant in the genus, surviving low humidity primarily by reducing its metabolic rate. Genetic control of reduced metabolic rate, however, has yet to be elucidated. We utilized the recently sequenced genome of D. mojavensis to create an oligonucleotide microarray to pursue the identities of the genes involved in metabolic regulation during desiccation. We observed large differences in gene expression between male and female D. mojavensis as well as both quantitative and qualitative sex differences in their ability to survive xeric conditions. As expected, genes associated with metabolic regulation and carbohydrate metabolism were differentially regulated between stress treatments. Most importantly, we identified four points in central metabolism (Glyceraldehyde 3-phosphate dehydrogenase, transaldolase, alcohol dehydrogenase, and phosphoenolpyruvate carboxykinase) that indicate the potential mechanisms controlling metabolic rate reduction associated with desiccation resistance. Furthermore, a large number of genes associated with vision pathways also were differentially expressed between stress treatments, especially in females, that may underlie the initial detection of stressful environments and trigger subsequent metabolic changes.

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Section: Discussionmentioning

confidence: 73%

Transcriptional Regulation of Metabolism Associated With the Increased Desiccation Resistance of the Cactophilic Drosophila mojavensis

Matzkin

Markow²

2009

Genetics

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“…Rogoff et al (1964) reported that benzene extracts of female Musca domestica (L.) elicited both attraction and excitation of mating behavior patterns in males. Carlson et al (1971) have found Z-9-tricosene as sex attractant for house fly; similarly, the versatile role of hydrocarbons in different insects such as stable fly (Muhammed et al 1975), little house fly (Uebel et al 1977), horn fly (Macklay 1977), tsetse fly (Carlson et al 1978;Huyton et al 1980;Carlson and Schlein 1991), and fruit fly (Oguma et al 1992a, b) has been elucidated. Moore and Fisher (1969) have reported that a metabolite released into the water by Ae.…”

Section: Introductionmentioning

confidence: 97%

Electroantennogram, flight orientation, and oviposition responses of Aedes aegypti to the oviposition pheromone n-heneicosane

Seenivasagan

Sharma

Sekhar³

et al. 2008

Parasitol Res

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Oviposition pheromones specifically influence the females of many insects to lay eggs in the sites resulting in more egg deposition. A previous report describes the principal role of n-heneicosane (C(21)) identified and characterized from the larval cuticle of Aedes aegypti (L.) in attracting the gravid mosquitoes to oviposit in treated substrates among other chemical components. However, the means by which this compound is perceived by the females for oviposition has not been reported. In this study, we have recorded the peripheral olfactory responses from the antenna of Ae. aegypti from 10(-7) g to 10(-3) g doses of n-heneicosane. The EAG response of female mosquitoes increased in a dose-dependent manner with increasing stimulus strength. In the orientation assay using Y-maze olfactometer, female mosquitoes were attracted to the odor plume of 10(-6) g and 10(-5) g dose, while the higher dose of 10(-3) g plume enforced repellency to gravid mosquitoes. The response to oviposition substrates by gravid Ae. aegypti females differed across the range of concentrations of n-heneicosane under multiple choice conditions, larger number of eggs were deposited in 10 ppm (10 mg/l) solutions compared to lower and higher concentrations indicating 10 ppm was most attractive. Application of n-heneicosane at 10 ppm in breeding habitats will be a useful method to attract the gravid mosquitoes using ovitraps for surveillance and monitoring. The possible use of this compound in monitoring of mosquito population in endemic areas in relevance to integrated vector management strategies is discussed in detail.

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“…The two pheromones, the non-volatile MSP and the volatile ASP, may function in a synergistic manner to enable the male to reach the female's gonopore. Perhaps the volatile sex pheromone enhances the effect of the contact sex pheromone just as vision, tactile stimuli or interactive behavior patterns enhance the contact sex pheromone perception in some insects (Carlson et al, 1978;Langley et al, 1982).…”

Section: Discussionmentioning

confidence: 99%

“…A number of studies have been conducted on the identification of the cuticular contact sex pheromones and their receptors in the African tsetse flies because of their importance as vectors of African sleeping sickness. Langley et al (1975) first described the cuticular contact sex pheromone of Glossina (morsitans) morsitans as 15, 19, 23-trimethyl-heptatriacontane; later, Carlson et al (1978) isolated, identified and synthesized the compound along with non-stimulatory components of the pheromone. Carlson et al (1978), Huyton et al (1980), Langley et al (1982) and Langley and Hall (1984) also noted that visual and tactile stimuli are important in the mating behavior of tsetse flies.…”

Section: Discussionmentioning

confidence: 99%

Role of the male claw sensilla in perception of female mounting sex pheromone in Dermacentor variabilis, Dermacentor andersoni and Amblyomma americanum

Phillips

Sonenshine

1993

Exp Appl Acarol

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Phillips, J. S. and Sonenshinc, D. l:'. Role of the male claw sensilla in perception of female mounting sex pheromone in Dermacentor variabilis, Dermacentor amlersoni and Amblyomma americanum. Exp. Appl. Acarol., 17: 631-653.The foreleg claw sensilla of male D. variabilis, D. andersoni and A. americam~m ticks include the receptors that perceive the female contact mounting sex pheromone (MSP). In all three tick species, the foreleg claw sensilla comprise six anteriorly-directed setae arranged in three symmetrical pairs, two each on the opposite sides of the apotele of the claw and one on the ventral side, Morphological study and behavioral bioassays of these setac revealed that only d~e dorsal ,and middle (= lateral) pairs of claw sensilla are mechanogustato~', while the ventral pair are strictly mechanoreceptors. The dorsal and middle sensou' setae exhibit a single pore-like structure located at or near their tip, a feature characteristic of mechanogustatory scnsilla. These setae are similar to those found on the palps that are believed to tunction as pheromone receptors, in all three tick species, male mounting and postmounting behaviors were suppressed only when the dorsal and middle pairs of claw sensilla were ablated or covered with gelatin; normal behavior was restored when the gelatin was removed. Doseresponse bioassays were conducted with D. variabilis males to authenticate the results of the gelatin tests. The results of these bioassays demonstrated that the gelatin coat was impervious to the pheromone. The characteristics of the ixodid tick mating system that distinguish it from mating processes in other arthropods are discussed.

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Sex Pheromone of the Tsetse Fly: Isolation, Identification, and Synthesis of Contact Aphrodisiacs

Cited by 144 publications

References 12 publications

Transcriptional Regulation of Metabolism Associated With the Increased Desiccation Resistance of the Cactophilic Drosophila mojavensis

Transcriptional Regulation of Metabolism Associated With the Increased Desiccation Resistance of the Cactophilic Drosophila mojavensis

Electroantennogram, flight orientation, and oviposition responses of Aedes aegypti to the oviposition pheromone n-heneicosane

Role of the male claw sensilla in perception of female mounting sex pheromone in Dermacentor variabilis, Dermacentor andersoni and Amblyomma americanum

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