SEX PHEROMONES THAT INDUCE SEXUAL CELL DIVISION IN THE CLOSTERIUM PERACEROSUM–STRIGOSUM–LITTORALE COMPLEX (CHAROPHYTA)1

Tsuchikane, Yuki; Fukumoto, Ryo-hei; Akatsuka, Satoko; Fujii, Tadashi; Sekimoto, Hiroyuki

doi:10.1046/j.1529-8817.2003.02062.x

Cited by 28 publications

(29 citation statements)

References 12 publications

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“…In addition, although many of the deduced amino acid sequences of the ESTs on the array have no similarity to known proteins, a number of interesting ESTs, such as those related to intercellular communication, receptor-like protein kinase (CpRLK1), and LRR-containing receptor-like protein (CpRLP1), were unearthed by data mining. In Closterium, intercellular communication between two mating types via pheromonal substances is essential for successful conjugation (Tsuchikane et al, 2003(Tsuchikane et al, , 2005. To date, direct information on the relationships between sex pheromones and these molecules has been lacking.…”

Section: Discussionmentioning

confidence: 99%

Gene Expression Profiling Using cDNA Microarray Analysis of the Sexual Reproduction Stage of the Unicellular Charophycean Alga Closterium peracerosum-strigosum-littorale Complex

Sekimoto

Tanabe²,

Tsuchikane³

et al. 2006

Plant Physiology

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The desmid Closterium peracerosum-strigosum-littorale complex, which is the closest unicellular sister to land plants, is the best characterized of the charophycean green algae with respect to the process of sexual reproduction. To elucidate the molecular mechanism of intercellular communication during sexual reproduction, we created a normalized cDNA library from mixed cells of the sexual and the vegetative phases and generated a cDNA microarray. In total, 3,236 expressed sequence tags, which were classified into 1,615 nonredundant groups, were generated for cDNA microarray construction. Candidate genes for key factors involved in fertilization, such as those that encode putative receptor-like protein kinase, leucine-rich-repeat receptorlike protein, and sex pheromone homologs, were up-regulated during sexual reproduction and/or by the addition of the purified sex pheromones, and the expression patterns of these genes were confirmed by quantitative real-time polymerase chain reaction analysis. This first transcriptome profile of Closterium will provide critical clues as to the mechanism and evolution of intercellular communication between the egg and sperm cells of land plants.

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Section: Discussionmentioning

confidence: 99%

Gene Expression Profiling Using cDNA Microarray Analysis of the Sexual Reproduction Stage of the Unicellular Charophycean Alga Closterium peracerosum-strigosum-littorale Complex

Sekimoto

Tanabe²,

Tsuchikane³

et al. 2006

Plant Physiology

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“…Two sets of primers that hybridize in the MADS or K domain of CpMADS1 were used. CpMADS1 expression gradually increased after cocultivation in nitrogendeficient medium was begun, with the highest expression at 4 h after the beginning of cocultivation, at which point the vegetative cells were communicating with the opposite mating-type cells and beginning to differentiate into gametangial cells (25). CpMADS1 expression decreased at Ϸ8 h after the onset of cocultivation, when most of the cells had begun to differentiate into gametangial cells but before fertilization (25).…”

Section: Expression Patterns Of Stonewort and Desmid Mads-box Genesmentioning

confidence: 99%

“…C. peracerosum-strigosum-littorale complex is a unicellular, heterothallic green alga. When the vegetative cells of two different mating types (mt Ϫ and mt ϩ ) are cocultured in nitrogen-deficient medium, the cells divide and differentiate into two gametangial cells (25). Gametangial cells of opposite mating types form a pair and release their gametes, which conjugate to form a zygote.…”

Section: Expression Patterns Of Stonewort and Desmid Mads-box Genesmentioning

confidence: 99%

Characterization of MADS-box genes in charophycean green algae and its implication for the evolution of MADS-box genes

Tanabe

Hasebe²,

Sekimoto³

et al. 2005

Proc. Natl. Acad. Sci. U.S.A.

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The MADS-box genes of land plants are extensively diverged to form a superfamily and are important in various aspects of development including the specification of floral organs as homeotic selector genes. The closest relatives of land plants are the freshwater green algae charophyceans. To study the origin and evolution of land plant MADS-box genes, we characterized these genes in three charophycean green algae: the stonewort Chara globularis, the coleochaete Coleochaete scutata, and the desmid Closterium peracerosum-strigosum-littorale complex. Phylogenetic analyses suggested that MADS-box genes diverged extensively in the land plant lineage after the separation of charophyceans from land plants. The stonewort C. globularis mRNA was specifically detected in the oogonium and antheridium together with the egg and spermatozoid during their differentiation. The expression of the C. peracerosum-strigosum-littorale-complex gene increased when vegetative cells began to differentiate into gametangial cells and decreased after fertilization. These expression patterns suggest that the precursors of land plant MADS-box genes originally functioned in haploid reproductive cell differentiation and that the haploid MADS-box genes were recruited into a diploid generation during the evolution of land plants.charophytes ͉ land plants ͉ Chara ͉ Coleochaete ͉ Closterium D iversity in form, a hallmark of extant species, is probably caused by modifications of ancestral gene networks regulating development and by the generation of novel developmental processes (1). The evolution of transcription factors, which have critical functions in development, through gene duplication and subsequent functional divergence has been hypothesized to be a major force in developmental evolution (reviewed in ref.2). The adaptation of green plants to a terrestrial environment and their subsequent diversification are tightly linked to the evolution of the body plan of land plants (3, 4). Members of the MADS-box gene family regulate various aspects of development in flowering plants and therefore were probably involved in the evolution of the morphology of land plants.MADS-box genes are characterized by the conserved MADS domain and are found in a wide range of eukaryotes including metazoans, fungi, slime mold, and green plants (5). These genes have been classified into several groups (6). MIKC C -and MIKC*-type MADS-box genes contain intervening (I), keratinlike (K), and C-terminal (C) domains (7,8). They are present in all major land plant taxa including seed plants, pteridophytes, and bryophytes but have not been found in other organisms such as green algae (5, 6, 9). The Arabidopsis genome (10) contains Ϸ38 MIKC C -type and 5 MIKC*-type genes (6, 9). The MIKC*-type genes tend to form a monophyletic group, including several subgroups of genes with unknown functions (9), but the MIKC Ctype MADS-box genes have been classified into about a dozen subfamilies with diverse functions and expression patterns (reviewed in ref. 6). The floral homeotic genes ar...

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“…The SCD-inducing activities specifi c to the two mating-type cells have been detected and characterized physiologically (Tsuchikane et al 2003 ). Closterium exhibits gliding locomotory behavior, mediated by the forceful extrusion of mucilage from one pole of the cell that causes the cell to glide in the opposite direction (Domozych et al 1993 ).…”

Section: Sex Pheromones Involved In Scd and Mucilage Secretionmentioning

confidence: 99%

Sexual Reproduction of a Unicellular Charophycean Alga, Closterium peracerosum-strogosum-littorale Complex

Sekimoto

Tsuchikane

Abe

2014

Sexual Reproduction in Animals and Plants

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The genus Closterium is the best characterized charophycean green alga with respect to the process of sexual reproduction. Two sex pheromones , named PR-IP Inducer and PR-IP , that are involved in the progress of these processes were physiologically and biochemically characterized and the corresponding genes were cloned. These pheromones function in most steps of sexual reproduction. The timing after mixing, appropriate concentrations of the pheromones, and conditions of the cells are all essential for pheromones to be functional. To elucidate the molecular mechanisms of sexual reproduction in detail, molecular tools such as expressed sequence tag (EST), microarray analysis, and genetic transformation systems have been established. These methods will enable us to clarify the details of sexual reproduction in the near future.

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SEX PHEROMONES THAT INDUCE SEXUAL CELL DIVISION IN THE CLOSTERIUM PERACEROSUM–STRIGOSUM–LITTORALE COMPLEX (CHAROPHYTA)¹

Cited by 28 publications

References 12 publications

Gene Expression Profiling Using cDNA Microarray Analysis of the Sexual Reproduction Stage of the Unicellular Charophycean Alga Closterium peracerosum-strigosum-littorale Complex

Gene Expression Profiling Using cDNA Microarray Analysis of the Sexual Reproduction Stage of the Unicellular Charophycean Alga Closterium peracerosum-strigosum-littorale Complex

Characterization of MADS-box genes in charophycean green algae and its implication for the evolution of MADS-box genes

Sexual Reproduction of a Unicellular Charophycean Alga, Closterium peracerosum-strogosum-littorale Complex

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