Sex-Related Effect on Gene Expression in the Mouse Meibomian Gland

Richards, Stephen M.; Yamagami, Hiroko; Schirra, F.; Suzuki, Tomo; Sullivan, David A.; Jensen, Roderick V.

doi:10.1080/02713680500514644

Cited by 24 publications

(20 citation statements)

References 38 publications

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“…Our findings are consistent with the gender associated and gland specific variations in mRNA levels previously reported in rodent model salivary gland systems (20,27,28) and in lacrimal (29) and meibomian glands (30). The differentially expressed genes in these studies are involved in a wide range of biological processes, molecular functions and cellular components, including growth and development, transcription, metabolism, signal transduction, ion transport, receptor activity and protein and nucleic acid binding.…”

Section: Discussionsupporting

confidence: 79%

Age and gender related differences in human parotid gland gene expression

Srivastava

Wang

Zhou

et al. 2008

Archives of Oral Biology

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Objective-The present study evaluated differences in gene expression associated with age and gender in the human parotid gland.Design-Parotid gland tissue was analyzed using the Affymetrix® GeneChip® HGU133plus2.0 array.Results-Differential gene expression, defined as a statistically significant difference with a 1.5 fold or greater change, was detected in 787 gene probe sets; 467 (~59%) showed higher expression in females. Several genes associated with saliva secretion were differentially expressed in male and female parotid glands including vesicle-associated membrane protein 3 VAMP3, synaptosomalassociated protein SNAP23, RAS oncogene family member RAB1A and the syntaxin binding protein STXBP1. Evaluation of gene expression in the youngest and the oldest female subjects revealed that the expression of 228 probe sets were altered during aging; 155 genes were up-regulated in the aged female parotid gland. However, of the genes that were altered during aging, 22 of the 30 probes (73%) classified as being associated with immune responses were down-regulated in the aged parotid gland. A panel of differentially expressed, age-and gender-related genes was selected for validation by quantitative, real-time RT-PCR. Comparable differences in gene expression were detected by both Affymetrix array and quantitative, real-time RT-PCR methods.Conclusions-Our data suggest that salivary gland function may be adversely affected in the aged population due, at least in part, to the altered regulation of several categories of genes. Moreover, the gender specific differences in gene expression identified in the present study correlate with the previously observed sexual dimorphism in salivary gland function.

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Section: Discussionsupporting

confidence: 79%

Age and gender related differences in human parotid gland gene expression

Srivastava

Wang

Zhou

et al. 2008

Archives of Oral Biology

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“…Recent studies have demonstrated that factors such as strain [34,35], gender [36,37], as well as diet [38,39] and circadian variation, [40] can influence gene expressions in various organisms and tissues. In our study we created two retina samples by randomized pooling of samples from a large number of individuals aiming to cancel out the effects of such factors.…”

Section: Discussionmentioning

confidence: 99%

Comparison of hybridization-based and sequencing-based gene expression technologies on biological replicates

et al. 2007

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Background: High-throughput systems for gene expression profiling have been developed and have matured rapidly through the past decade. Broadly, these can be divided into two categories: hybridization-based and sequencing-based approaches. With data from different technologies being accumulated, concerns and challenges are raised about the level of agreement across technologies. As part of an ongoing large-scale cross-platform data comparison framework, we report here a comparison based on identical samples between one-dye DNA microarray platforms and MPSS (Massively Parallel Signature Sequencing).

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“…[125][126][127] Androgens appear to control 30% of the sexassociated variations in sex-related differences of the MG (in mouse). 128 Androgens have a major influence on MG structure, 129,130 aging and androgen status disorders leading to obstructive MGD with a lack of lipids at the lid margin and in the tear film, an altered lipid profile, and DE symptoms. 15,79,[131][132][133] Estrogens (in particular 17-b-estradiol) appear to negatively influence lipid secretion in MG, by suppressing genes involved in lipid biosynthesis, whereas they upregulate the expression of genes involved in lipid and fatty acid catabolism.…”

Section: Gender Sex Steroids and Evaporative Dementioning

confidence: 99%

Sex-Steroid Imbalance in Females and Dry Eye

Versura

Giannaccare

Campos

2014

Current Eye Research

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Dry eye (DE) is a multifactorial disorder of the ocular surface unit that results in eye discomfort, visual disturbance and ocular surface damage. It is one of the most common complaints in daily ophthalmic practice. The risk of DE increases with age in both sexes, while its incidence is higher among females. In addition, the condition of menopause in aging women may also contribute to DE onset or worsening as a consequence of an overall hormonal imbalance. Sex hormones play a key role in ocular surface physiology and they impact differently on ocular surface tissues. Reduced estrogen levels were historically thought to be responsible in age-related DE onset but more recent investigations have reconsidered the role of androgens that are present and exert a protective function on the ocular surface. Hormone levels themselves, withdrawal changes in hormone levels, and the changes in hormone-receptor responsiveness are all important factors but it remains to be fully elucidated how estrogen or androgen insufficiency act alone or together in a combined imbalance or interplay to raise the risk of disease. The purpose of this review is to briefly outline current scientific evidence on the influence of androgens and estrogens, on the Lachrymal and Meibomian glands and on ocular surface epithelia including conjunctival goblet cells during reproductive and menopausal periods. The role of sex steroids is also discussed in relation to the pathogenesis of different forms of DE and Sjogren's syndrome (SS). The impact of systemic hormone therapy (HT) in DE post-menopausal women still appears as a controversial issue, despite the many clinical studies. Finally, the outcomes of topical applications of steroid-based products are summarized, underlying the need for potential (tear) biomarker(s) in the rationale of DE-targeted therapy.

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Sex-Related Effect on Gene Expression in the Mouse Meibomian Gland

Abstract: These findings support our hypothesis that sex-related differences exist in gene expression of the meibomian gland.

Cited by 24 publications

References 38 publications

Age and gender related differences in human parotid gland gene expression

Age and gender related differences in human parotid gland gene expression

Comparison of hybridization-based and sequencing-based gene expression technologies on biological replicates

Sex-Steroid Imbalance in Females and Dry Eye

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