Sex-specific expression of CTNNB1 in the gonadal morphogenesis of the chicken

Bae, Seung Min; Lim, Whasun; Jeong, Wooyoung; Lee, Jin Young; Kim, Jin‐Young; Bazer, Fuller W.; Song, Gwonhwa

doi:10.1186/1477-7827-11-89

Cited by 14 publications

(8 citation statements)

References 41 publications

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“…The cyto-locations of β-catenin in the gonads vary among species. In mice, β-catenin mRNA is mainly expressed in ovarian somatic cells, such as granulosa cells and surface epithelium cells [ 34 ]. In C .…”

Section: Discussionmentioning

confidence: 99%

“…semilaevis , Nile tilapia and Chlamys farreri , β-catenin is predominately expressed in the oocytes, especially in the early stages of development [ 12 , 27 ]. However, chicken β-catenin mRNA is transcribed only in the Sertoli cells of the testes from the immature to mature stages [ 34 ]. We speculate that this restricted transcription is probably caused by differences in the species.…”

Section: Discussionmentioning

confidence: 99%

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Identification and analysis of the β-catenin1 gene in half-smooth tongue sole (Cynoglossus semilaevis)

Zhu

et al. 2017

PLoS ONE

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β-catenin is a key signalling molecule in the canonical Wnt pathway, which plays a role in cell adhesion, embryogenesis and sex determination. However, little is known about its function in teleosts. We cloned and characterized the full-length β-catenin1 gene from half-smooth tongue sole (Cynoglossus semilaevis), which was designated CS-β-catenin1. The CS-β-catenin1 cDNA consists of 2,346 nucleotides and encodes a protein with 782 amino acids. Although CS-β-catenin1 was transcribed in the gonads of both sexes, the level was significantly higher in ovaries compared to testes. Furthermore, the mRNA level of CS-β-catenin1 was significantly upregulated at 160 days and constantly increased until 2 years of age. In situ hybridization revealed that CS-β-catenin1 mRNA was mainly localized in oocyte cells, especially in stage I, II and III oocytes. When CS-β-catenin1 expression was inhibited by injection of quercetin in the ovaries, levels of CS-Figla and CS-foxl2 mRNA were significantly down-regulated, and CS-dmrt1 was up-regulated, which suggested that CS-β-catenin1 is a potential upstream gene of CS-Figla and is involved in the development of the ovaries, i.e., folliculogenesis.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Identification and analysis of the β-catenin1 gene in half-smooth tongue sole (Cynoglossus semilaevis)

Zhu

et al. 2017

PLoS ONE

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“…Chlamys farreri, b-catenin was localized to the oocytes of the ovary (Li et al, 2014). In mammals, b-catenin was found in both germ and somatic cells of the ovary in mouse (Liu et al, 2009); But little or no expression of b-catenin mRNA was detected in the either immature or mature ovary (Bae et al, 2013). In this study, the b-catenin 1 protein was concentrated mainly in the cytoplasm of early development Oestrogen, especially E2, plays an important role in gonad development, sex regulation and reproductive behaviour.…”

Section: Discussionmentioning

confidence: 99%

“…β ‐catenin is the transcriptional effector of canonical Wnt signalling pathway, which plays an important role in sex determination and differentiation of mammals (Eggers & Sinclair, ). Currently, researches show that the activity of β ‐catenin gene had been determined as a pivotal gene for gonad development and maintenance of ovarian function in mammalian species (Bae et al., ). β ‐catenin loss leads to the formation and appearance of testis‐specific cells in foetal mouse ovaries (Liu, Bingham, Parker & Yao, ).…”

Section: Introductionmentioning

confidence: 99%

Characterization of β-catenin 1 during the gonad development in the common carp (Cyprinus carpio )

et al. 2017

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β‐catenin gene is a pivotal gene for gonad development and maintenance of ovarian function in mammals. However, little is known about its expression and function in gonad development of fish. In this study, a complete cDNA (3342 bp) sequence of β‐catenin 1 was cloned from the common carp, Cyprinus carpio, by RACE PCR, which encodes a 780‐amino‐acid protein. Quantitative real‐time PCR demonstrated that β‐catenin 1 mRNA expressions were high in the testis and ovary tissue and the expression increased as the testes developed and the early stage ovaries developed. Western blot results revealed a single immunoreactive band with an estimated molecular weight of 90 kDa in testes. Immunohistochemistry analysis revealed that the β‐catenin 1 protein was concentrated mainly in the cytoplasm of early development stage of oocyte cells and in the cytomembrane of developing and mature sperm cells. 17β‐Ethinylestradiol injecting intraperitoneally into the fish decreased the relative β‐catenin 1 mRNA expression level except 1 μg/g 72 hr and 5 μg/g 48 hr of treatments in the ovary by real‐time PCR. These results suggest, for the first time, that β‐catenin 1 is an essential protein in gonad development and might be involved in ovarian early development of C. carpio.

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“…JUN and FOS, core nodes in module 1 of focally-irradiated lung areas, are transcription factors involved in diverse cellular processes such as development and immune process [ 45 , 46 ]. CTNNB1, core node with degrees of 51 in module 3 of focally-irradiated lung areas, is a key transcriptional regulator and is involved in morphogenesis or developmental process [ 47 , 48 ]. CTNNB1 was reported to regulate immune response [ 49 ] and to integrate cell adhesion and differentiation [ 50 ].…”

Section: Discussionmentioning

confidence: 99%

Focal exposure of limited lung volumes to high-dose irradiation down-regulated organ development-related functions and up-regulated the immune response in mouse pulmonary tissues

Kim

Jin²,

Lee

et al. 2016

BMC Genet

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BackgroundDespite the emergence of stereotactic body radiotherapy (SBRT) for treatment of medically inoperable early-stage non-small-cell lung cancer patients, the molecular effects of focal exposure of limited lung volumes to high-dose radiation have not been fully characterized. This study was designed to identify molecular changes induced by focal high-dose irradiation using a mouse model of SBRT.ResultsCentral areas of the mouse left lung were focally-irradiated (3 mm in diameter) with a single high-dose of radiation (90 Gy). Temporal changes in gene expression in the irradiated and non-irradiated neighboring lung regions were analyzed by microarray. For comparison, the long-term effect (12 months) of 20 Gy radiation on a diffuse region of lung was also measured. The majority of genes were down-regulated in the focally-irradiated lung areas at 2 to 3 weeks after irradiation. This pattern of gene expression was clearly different than gene expression in the diffuse region of lungs exposed to low-dose radiation. Ontological and pathway analyses indicated these down-regulated genes were mainly associated with organ development. Although the number was small, genes that were up-regulated after focal irradiation were associated with immune-related functions. The temporal patterns of gene expression and the associated biological functions were also similar in non-irradiated neighboring lung regions, although statistical significance was greatly reduced when compared with those from focally-irradiated areas of the lung. From network analysis of temporally regulated genes, we identified inter-related modules associated with diverse functions, including organ development and the immune response, in both the focally-irradiated regions and non-irradiated neighboring lung regions.ConclusionsFocal exposure of lung tissue to high-dose radiation induced expression of genes associated with organ development and the immune response. This pattern of gene expression was also observed in non-irradiated neighboring areas of lung tissue, indicating a global lung response to focal high-dose irradiation.Electronic supplementary materialThe online version of this article (doi:10.1186/s12863-016-0338-9) contains supplementary material, which is available to authorized users.

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Sex-specific expression of CTNNB1 in the gonadal morphogenesis of the chicken

Cited by 14 publications

References 41 publications

Identification and analysis of the β-catenin1 gene in half-smooth tongue sole (Cynoglossus semilaevis)

Identification and analysis of the β-catenin1 gene in half-smooth tongue sole (Cynoglossus semilaevis)

Characterization of β-catenin 1 during the gonad development in the common carp (Cyprinus carpio )

Focal exposure of limited lung volumes to high-dose irradiation down-regulated organ development-related functions and up-regulated the immune response in mouse pulmonary tissues

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