Sex-specific responses of the reproductive system of zebrafish (Danio rerio) to electrolysis

Zheng, Chao; Chen, Cheng; Gao, Yan; Gan, Lin; Zhang, Wen; Yang, Liuyan

doi:10.1016/j.cbpc.2022.109294

Cited by 2 publications

(2 citation statements)

References 47 publications

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“…Indeed, regarding Experiment 1, cancer does not appear to be associated with differential sex-specific mortality at the embryonic stage, nor does it play a role in sex determination (since both phenomena would have skewed the sex-ratio). These results contrasts with numerous studies that have identified sex-specific responses in zebrafish regarding various factors (e.g., (Zhou et al 2010, Li et al 2019, Giommi et al 2022, Lin et al 2022, Park et al 2022, van Gelderen et al 2022, Zhai et al 2022, Zheng et al 2022, Zhong et al 2023)), including cancers (e.g., (Lee et al 2018, 2021, Mensah et al 2019, Montal et al 2023)). One possible explanation is that the cancer type used in our study may have too low aggressiveness, as possibly evidenced by the ability of individuals affected at the embryo stage to develop into adulthood.…”

Section: Discussioncontrasting

confidence: 99%

Consequences of Cancer on ZebrafishDanio rerio: Insights into Sex Determination, Sex Ratio, and Offspring Survival

Boutry,

Douhard,

Asselin

et al. 2024

Preprint

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Offspring sex ratio has been proposed as an indicator of the risk of developing certain cancers in humans, but offspring sex ratio may also be a consequence of the disease. In this study, we delve into this subject using the fish Danio rerio as a model system. First, we explore whether inducing skin cancer at an early stage of the host's life (embryonic stage) has the potential to influence sex determination and/or sex-specific mortality. Second, we investigate whether the sex ratio in offspring produced by tumor-bearing adult females differs from that of healthy females. Third, we compare the survival (until sexual maturity) of offspring produced by cancerous and non-cancerous females. We found that skin cancer did not influence sex ratio in both experiments. However, consistent with previous studies on other model systems, the survival of offspring from cancerous females was higher, suggesting that diseased females allocate more resources to current reproductive efforts compared to their healthy counterparts. This study makes a significant contribution to our understanding of the ecological and evolutionary consequences of host-tumor interactions in animals.

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Section: Discussioncontrasting

confidence: 99%

Consequences of Cancer on ZebrafishDanio rerio: Insights into Sex Determination, Sex Ratio, and Offspring Survival

Boutry,

Douhard,

Asselin

et al. 2024

Preprint

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“…The expressions of estrogen synthesis genes cyp19a1b and receptor gene esr1 were significantly upregulated with the P and IP treatment (Figure C). Vitellogenin (VTG) is synthesized primarily in the liver of female fish under the control of the steroid hormone, and abnormal production of VTG is considered a specific biomarker for hormone-mimicking pollutants in the environment. , The production of E2 and VTG was stimulated by cyp19a1b . As expected, increases were noted in the levels of E2 and VTG.…”

Section: Resultsmentioning

confidence: 99%

Psoralen and Isopsoralen, Two Estrogen-Like Natural Products from Psoraleae Fructus, Induced Cholestasis via Activation of ERK1/2

Chen,

Qian,

et al. 2024

Chem. Res. Toxicol.

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With the increasing use of oral contraceptives and estrogen replacement therapy, the incidence of estrogen-induced cholestasis (EC) has tended to rise. Psoralen (P) and isopsoralen (IP) are the major bioactive components in Psoraleae Fructus, and their estrogen-like activities have already been recognized. Recent studies have also reported that ERK1/2 plays a critical role in EC in mice. This study aimed to investigate whether P and IP induce EC and reveal specific mechanisms. It was found that P and IP increased the expression of esr1, cyp19a1b and the levels of E2 and VTG at 80 μM in zebrafish larvae. Exemestane (Exe), an aromatase antagonist, blocked estrogen-like activities of P and IP. At the same time, P and IP induced cholestatic hepatotoxicity in zebrafish larvae with increasing liver fluorescence areas and bile flow inhibition rates. Further mechanistic analysis revealed that P and IP significantly decreased the expression of bile acids (BAs) synthesis genes cyp7a1 and cyp8b1, BAs transport genes abcb11b and slc10a1, and BAs receptor genes nr1h4 and nr0b2a. In addition, P and IP caused EC by increasing the level of phosphorylation of ERK1/2. The ERK1/2 antagonists GDC0994 and Exe both showed significant rescue effects in terms of cholestatic liver injury. In conclusion, we comprehensively studied the specific mechanisms of P- and IP-induced EC and speculated that ERK1/2 may represent an important therapeutic target for EC induced by phytoestrogens.

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Sex-specific responses of the reproductive system of zebrafish (Danio rerio) to electrolysis

Cited by 2 publications

References 47 publications

Consequences of Cancer on ZebrafishDanio rerio: Insights into Sex Determination, Sex Ratio, and Offspring Survival

Consequences of Cancer on ZebrafishDanio rerio: Insights into Sex Determination, Sex Ratio, and Offspring Survival

Psoralen and Isopsoralen, Two Estrogen-Like Natural Products from Psoraleae Fructus, Induced Cholestasis via Activation of ERK1/2

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