Sex Steroids Level in Blood Plasma and Ovarian Follicles of the Chimeric Chicken

Sechman, Andrzej; Łakota, Paweł; Wojtysiak, Dorota; Hrabia, Anna; Mika, Magdalena; Lisowski, Mirosław; Czekalski, P.; Rzasa, J; Kapkowska, E.; Bednarczyk, Marek

doi:10.1111/j.1439-0442.2006.00886.x

Cited by 18 publications

(12 citation statements)

References 43 publications

(61 reference statements)

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“…These results were in agreement with expectations, especially the high rate of gonad chimerism observed in progeny tested chimeras produced using the same method (BEDNARCZYK et al 2002;SECHMAN et al 2006). Gonads are the final destination for PG.…”

Section: Discussionsupporting

confidence: 92%

Identification of the Rate of Chimerism of Different Tissues with Microsatellite Markers in Chicken Chimeras

Siwek

Sławińska²,

Łakota³

et al. 2010

folia biol (krakow)

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The goal of our study was to evaluate whether private alleles can be defined in microsatellite markers for the breeds under investigation; to evaluate if these private alleles distinguish chicken chimera when using different tissues; to trace them back to the donor: Green-Legged Partridgelike and recipient: White Leghorn chicken breeds, and further on, to estimate the level of chimerism in each tissue. Private and common alleles were defined for donor and recipient chicken breeds in 3 loci. The rate of chimerism was defined based on private alleles present in liver, heart, breast muscle, femoral muscle and gonads. The highest rate of chimerism was observed in liver. A lower rate of chimersim was observed in gonads, and femoral muscle, and finally the lowest rate of chimerism was observed in breast muscle and heart.

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Section: Discussionsupporting

confidence: 92%

Identification of the Rate of Chimerism of Different Tissues with Microsatellite Markers in Chicken Chimeras

Siwek

Sławińska²,

Łakota³

et al. 2010

folia biol (krakow)

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“…The wall of the ovarian follicle consists of concentric layers of tissue that surround the oocyte and yolk, including the granulosa and the theca (interna and externa) (Johnson, 2000). The granulosa layer produces predominantly progesterone (P4) which is a precursor for testosterone (T) and oestradiol (E2) synthesis by the theca interna and externa layers, respectively (Bahr et al, 1983;Nitta et al, 1991;Rząsa et al, 2002;Sechman et al, 2004bSechman et al, , 2006. The stromal tissue with cortical follicles below 1 mm and the theca layer of white nonhierarchical follicles (< 8 mm), via the Δ 5 steroidogenic pathway, produce mainly T which is immediately converted to E2 by aromatase P450 (P450arom) (Lee et al, 1998).…”

mentioning

confidence: 99%

Effect of tamoxifen on sex steroid concentrations in chicken ovarian follicles

Rzasa

Sechman

Paczoska-Eliasiewicz

et al. 2009

Acta Veterinaria Hungarica

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The aim of the present investigation was to study the effect of tamoxifen (TAM), an oestrogen receptor antagonist, on the concentrations of sex hormones in chicken ovarian follicles. The experiment was carried out on Hy-line hens which were randomly divided into two groups (control and experimental). TAM was given at a dose of 4 mg/hen (per os) at first once a day for 7 consecutive days, and subsequently four times a day for the next 6 days. Control hens received placebo. Birds were killed on the day after the last TAM treatment. From the dissected ovaries the following compartments were isolated: stroma with follicles < 1 mm, white non-hierarchical (1-4 mm and 4-8 mm) and yellow hierarchical follicles (F6-F1; 18-35 mm). The concentrations of the sex steroids progesterone (P4), testosterone (T) and oestradiol (E2) in the ovarian follicles were determined by radioimmunoassay. In the TAM-treated group, a gradual decrease in egglaying rate was observed from the 4th day of the experiment. Eventually, egg laying stopped entirely on the 12th day of the experiment. TAM significantly decreased the weight of the ovary and affected the sex hormone concentrations in the ovarian follicles. Following TAM treatment (1) a significant increase in E2 and T concentrations in the stroma, white follicles and the F4 and F1 follicles, (2) a significant decrease in E2 and T concentrations in the F2 follicle, and (3) a significant decline of P4 in the F4 to F1 follicles were observed. The results indicate that the blockade of oestrogen receptors by TAM significantly modulates the process of chicken ovarian steroidogenesis.

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“…The liver (Liv) was used as a negative control; nd -not detected: a, b -significant at P#0.05. (BAHR et al 1983;ROBINSON & ETCHES 1986;GOMEZ et al 1998;LEE et al 1998;PROSZKO-WIEC-WÊGLARZ et al 2005;RZ¥SA et al 2009;SECHMAN et al 2006;SECHMAN et al 2009). Histochemical and immunohistochemical studies have also confirmed that granulosa cells of preovulatory follicles show 3â-HSD activity, which means that they are a site of steroid biosynthesis (ARMSTRONG 1979;DAVIDSON et al 1979;MARRONE & SEBRING 1989;NITTA et al 1993).…”

Section: Resultsmentioning

confidence: 97%

“…The structure and function of granulosa cells from preovulatory ovarian follicles, which are the principal source of progesterone (P 4 ) in avian ovaries, and their role in ovarian follicle growth has been the subject of several studies (ARMSTRONG 1979;BAHR et al 1983;GOMEZ et al 1998;NITTA et al 1993;YOSHIMURA et al 1993;YAO & BAHR 2001a;PROSZKOWIEC-WÊGLARZ et al 2005;RZ¥SA et al 2009;SECHMAN et al 2006;SECHMAN et al 2009). Most of these studies regarded the granulosa layer as a uniform structure.…”

mentioning

confidence: 99%

Structure and Steroidogenic Activity of the Granulosa Layer of F1 Preovulatory Ovarian Follicles of the Hen (Gallus domesticus)

Wojtysiak¹,

Okólski²,

Sechman³

2011

folia biol (krakow)

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WOJTYSIAK D., OKÓLSKI A., SECHMAN A. 2011. Structure and steroidogenic activity of the granulosa layer of F1 preovulatory ovarian follicles of the hen (Gallus domesticus). Folia biologica (Kraków) 59: 59-64. The study was performed to determine the structure and steroidogenic activity of granulosa cells derived from the germinal disc region, proximal region and distal region of the largest preovulatory ovarian follicle (F1) of the hen. The study was carried out on 34 Hy-Line Brown egg-laying hens aged 40 weeks. Morphology of the granulosa cells was studied by histological assessment and scanning electron microscopy. Moreover, the level of P " , histochemical activity of 3â-HSD and expression of 3â-HSD gene mRNA in granulosa cells of F1 follicle were determined. The findings indicate that the morphology and steroidogenic activity of the granulosa layer in F1 preovulatory ovarian follicle are associated with the region of the follicle. This is consistent with earlier studies. In the germinal disc region the granulosa cells form a multilayer while in the proximal and distal regions granulosa cells form a single layer. Analysis of P " concentration revealed that its level in granulosa cells was markedly reduced closer to the germinal disc. Moreover, our study demonstrates for the first time the lower histochemical activity of 3â-HSD and expression of 3â-HSD mRNA in granulosa cells from the germinal disc region compared with the proximal and distal region.

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Sex Steroids Level in Blood Plasma and Ovarian Follicles of the Chimeric Chicken

Cited by 18 publications

References 43 publications

Identification of the Rate of Chimerism of Different Tissues with Microsatellite Markers in Chicken Chimeras

Identification of the Rate of Chimerism of Different Tissues with Microsatellite Markers in Chicken Chimeras

Effect of tamoxifen on sex steroid concentrations in chicken ovarian follicles

Structure and Steroidogenic Activity of the Granulosa Layer of F1 Preovulatory Ovarian Follicles of the Hen (Gallus domesticus)

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