Sexing Greater Flamingo Chicks from Feather Bulb DNA

Balkız, Özge; Dano, Stéphanie; Barbraud, Christophe; Tekin, Serpil; Özesmi, Uygar; Dündar, Munis; Béchet, Arnaud

doi:10.1675/1524-4695(2007)030[0450:sgfcff]2.0.co;2

Cited by 17 publications

(17 citation statements)

References 14 publications

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“…We confirmed the putative sex of sexually monomorphic hosts using a PCR-based standard sexing technique that relies on the different lengths of introns found in the CHD-W and CHD-Z genes (Fridolfsson and Ellegren, 1999;Balkiz et al, 2007). In the case of the amblyceran and ischnoceran parasites found on the frigatebirds, we confirmed the species identification using a mitochondrial genetic marker.…”

Section: Molecular Analysissupporting

confidence: 64%

Comparative Ectoparasite Loads of Five Seabird Species in the Galapagos Islands

Rivera-Parra

Levin

Parker

2014

Journal of Parasitology

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In this paper we describe the ectoparasitic lice (Insecta: Phthiraptera) found on 5 species of seabirds (magnificent frigatebird Fregata magnificens; great frigatebird Fregata minor ; Nazca booby Sula granti ; blue-footed booby Sula nebouxii ; and red-footed booby Sula sula ) on the Galapagos Archipelago. We found 9 species of ectoparasitic lice: 5 species of Pectinopygus ischnocerans, 1 infesting each host; 2 species of Colpocephalum amblyceran lice, 1 on each frigatebird species; and 2 shared amblycerans, Eidmanniella albescens (Piaget, 1880) found on Nazca and blue-footed boobies and Fregatiella aurifasciata (Kellogg, 1899) found on the 2 frigatebirds. We tested the relative importance and interactions of host sex, body size, host, island, host family, and breeding status and found that inter-island differences were the main predictors of prevalence and infestation intensity. These differences could be related to host density or weather, but further evidence is needed.

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Section: Molecular Analysissupporting

confidence: 64%

Comparative Ectoparasite Loads of Five Seabird Species in the Galapagos Islands

Rivera-Parra

Levin

Parker

2014

Journal of Parasitology

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“…Molecular sexing was based on CHD gene sequences polymorphism and was carried out as described by either Bertault et al. (1999) or Balkız et al. (2007).…”

Section: Methodsmentioning

confidence: 99%

“…The quality and con-centration of DNA extracted was estimated by UV spectrophotometry (Spectramax plus 384, Molecular devices) to ensure that a final dilution of approximately 50 ng ⁄ lL was achieved. Molecular sexing was based on CHD gene sequences polymorphism and was carried out as described by either Bertault et al (1999) or Balkız et al (2007).…”

Section: Dna Extraction and Sexingmentioning

confidence: 99%

Genetic polymorphism in dopamine receptor D4 is associated with early body condition in a large population of greater flamingos, Phoenicopterus roseus

Gillingham¹,

Béchet²,

Geraci³

et al. 2012

Molecular Ecology

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Body condition is an important determinant of fitness in many natural populations. However, as for many fitness traits, the underlying genes that regulate body condition remain elusive. The dopamine receptor D4 gene (DRD4) is a promising candidate as dopamine is known to play an important role in the regulation of food intake and the metabolism of both glucose and lipids in vertebrates. In this study, we take advantage of a large data set of greater flamingos, Phoenicopterus roseus, to test whether DRD4 polymorphism predicts early body condition (EBC) while controlling for whole-genome effects of inbreeding and outbreeding using microsatellite multilocus heterozygosity (MLH). We typed 670 of these individuals for exon 3 of the homologue of the human DRD4 gene and 10 microsatellite markers. When controlling for the effects of yearly environmental variations and differences between sexes, we found strong evidence of an association between exon 3 DRD4 polymorphisms and EBC, with 2.2-2.3% of the variation being explained by DRD4 polymorphism, whereas there was only weak evidence that MLH predicts EBC. Because EBC is most likely a polygenic trait, this is a considerable amount of variation explained by a single gene. This is to our knowledge, the first study to show an association between exon 3 DRD4 polymorphism and body condition in non-human animals. We anticipate that the DRD4 gene as well as other genes coding for neurotransmitters and their receptors may play an important role in explaining variation in traits that affect fitness.

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“…This method requires the capture of birds for sampling blood, feather or saliva, although some advanced techniques allow gene samples to be taken from materials obtained noninvasively, such as molt feathers, feces and the inner layer of egg shells (Jensen et al 2003;Regnaut et al 2006;Balkiz et al 2007;Mudrik et al 2013). However, accurate genetic sexing with noninvasive samples requires highly qualified staff and methods have to be adapted for any particular species (Beja-Pereira et al 2009).…”

Section: Introductionmentioning

confidence: 99%

Gender identification using acoustic analysis in birds without external sexual dimorphism

Volodin

Volodina²,

Klenova

et al. 2015

Avian Res

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Zoo and wildlife management faces a problem with bird sexing, as many bird taxa have indiscernible gender differences in size and coloration. Problematic groups are geese, cranes, rails, raptors, owls, parrots, doves, auks, shearwaters and some passerines. Commonly accepted invasive sexing techniques based on genetics, laparoscopy, morphometric and on cloacal inspection, are all needed in bird capturing and handling. Capturing and subsequent manipulations may be inapplicable for free-ranging birds, whereas distant voice-based sexing is relevant for many species. This review evaluates the potential for noninvasive sexing by separate calls or duet calls, for adult birds of 69 species from 16 orders and for chicks of 11 species from 7 orders. For adult birds of 25 species, a single call per individual was sufficient for 100 % reliable sexing by ear or using spectrographic analysis. For chicks, the potential for voice-based sexing seems to be very limited. For birds calling rarely or unpredictably, we propose a simple way of provoking vocalization using playbacks of species-specific calls that are available from sound libraries. We conclude that sexing by voice may represent a feasible alternative to the classical sexing techniques, both in the wild and in captivity.

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Sexing Greater Flamingo Chicks from Feather Bulb DNA

Cited by 17 publications

References 14 publications

Comparative Ectoparasite Loads of Five Seabird Species in the Galapagos Islands

Comparative Ectoparasite Loads of Five Seabird Species in the Galapagos Islands

Genetic polymorphism in dopamine receptor D4 is associated with early body condition in a large population of greater flamingos, Phoenicopterus roseus

Gender identification using acoustic analysis in birds without external sexual dimorphism

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