Sexing live mountain pine beetles <i><scp>D</scp>endroctonus ponderosae</i>: refinement of a behavioral method for <i><scp>D</scp>endroctonus</i> spp.

Rosenberger, Derek W.; Venette, Robert C.; Aukema, Brian H.

doi:10.1111/eea.12463

Cited by 14 publications

(11 citation statements)

References 27 publications

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“…Within our own analysis, beetles were not sexed prior to genotyping due to the high proportion of larval individuals. Traditional MPB sexing methods (i.e., stridulation and seventh tergite morphology; Lyon, ) are time‐consuming and have some degree of inaccuracy (Rosenberger et al, ). Both methods call for undamaged adult beetles, but stridulation, a behavioral indicator, further requires specimens to be alive.…”

Section: Discussionmentioning

confidence: 99%

“…Further details concerning wild and laboratory‐bred specimens are given in Trevoy et al (). To aid in the molecular identification of sex‐related markers, the 13 offspring from laboratory crosses were morphologically sexed by inspection of the sclerotized plectrum found on the beetle's seventh abdominal tergite (Lyon, ; Rosenberger, Venette, & Aukema, ; Safranyik & Carroll, ).…”

Section: Methodsmentioning

confidence: 99%

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Repurposing population genetics data to discern genomic architecture: A case study of linkage cohort detection in mountain pine beetle (Dendroctonus ponderosae)

Trevoy

Janes

Muirhead

et al. 2018

Ecology and Evolution

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Genetic surveys of the population structure of species can be used as resources for exploring their genomic architecture. By adjusting filtering assumptions, genome‐wide single‐nucleotide polymorphism (SNP) datasets can be reused to give new insights into the genetic basis of divergence and speciation without targeted resampling of specimens. Filtering only for missing data and minor allele frequency, we used a combination of principal components analysis and linkage disequilibrium network analysis to distinguish three cohorts of variable SNPs in the mountain pine beetle in western Canada, including one that was sex‐linked and one that was geographically associated. These marker cohorts indicate genomically localized differentiation, and their detection demonstrates an accessible and intuitive method for discovering potential islands of genomic divergence without a priori knowledge of a species’ genomic architecture. Thus, this method has utility for directly addressing the genomic architecture of species and generating new hypotheses for functional research.

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Repurposing population genetics data to discern genomic architecture: A case study of linkage cohort detection in mountain pine beetle (Dendroctonus ponderosae)

Trevoy

Janes

Muirhead

et al. 2018

Ecology and Evolution

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“…Overall, the experimental group consisted of 124 female beetles, including 53 collected from site 1 bolts and 71 from site 2 bolts. Individual beetles were separated by sex based on the incidence of beetle stridulation, which is considered to be approxiately 98% accurate for identifying males (Rosenberger et al ., ). This was performed at 24 ± 1 °C.…”

Section: Methodsmentioning

confidence: 97%

Morphological variation associated with dispersal capacity in a tree‐killing bark beetleDendroctonus ponderosaeHopkins

Shegelski

Evenden

Sperling

2018

Agri and Forest Entomology

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1 Intrinsic factors influencing the dispersal of insect pests during outbreaks are poorly understood, yet these factors need to be quantified to parameterize dispersal in models that predict population spread. The present study related wing and body morphology of female mountain pine beetles (Dendroctonus ponderosae) to flight distance, as measured by flight mill bioassays. 2 Beetles that flew long distances (> 11 km) had a greater body weight and larger wings than beetles that flew short distances (< 1 km). These heavier female beetles should also be more capable pioneers because other studies have shown that body weight is positively correlated with lipid content. 3 Wing and body morphology of females are significant predictors of flight distance; heavy beetles with large wings generally flew further than smaller beetles, although this relationship is heteroscedastic. Dispersion of flight distance values increases with wing loading (weight/wing area) as a result of a cohort of 'lazy' individuals that fly short distances (< 1 km) regardless of flight phenotype. 4 The observed morphology explained less than 20% of the variation in flight capacity, indicating a substantial contribution from other intrinsic factors that remain to be investigated. The present study may have implications for dispersal modelling, providing estimates of flight capacity using morphological measurements.

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“…The ends of the bolts were sealed with paraffin wax to prevent desiccation, and the bolts were placed in cages (2m × 2m × 1m wooden boxes, with 0.5 mm screen side panels) at room temperature until beetles completed their development and emerged (~4–6 weeks). Beetles were collected from the sides and top of the cages, segregated by sex based upon stridulation [ 45 , 46 ], and either assigned to an endemic or epidemic population state simulation as described below.…”

Section: Methodsmentioning

confidence: 99%

Breeding matters: Natal experience influences population state-dependent host acceptance by an eruptive insect herbivore

Burke

Carroll

2017

PLoS ONE

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Eruptive forest insects are highly influential agents of change in forest ecosystems, and their effects have increased with recent climate change. State-dependent life histories contribute significantly to the population dynamics of eruptive forest insect herbivores; however, the proximate mechanisms by which these species shift between states is poorly understood. Laboratory bioassays were conducted using the mountain pine beetle (Dendroctonus ponderosae) to determine the effect of maternal host selection on offspring host preferences, as they apply to population state-dependent behaviors. Female mountain pine beetles exhibited state-dependent preference for artificial host material amended with monoterpenes in the absence of other cues, such that individuals reared in high-density epidemic-state simulations rejected low monoterpene conditions, while low-density endemic-state beetles accepted low monoterpene conditions. State-specific behavior in offspring was dependent on rearing conditions, as a function of maternal host selection, and these effects were observed within one generation. Density-dependent host selection behaviors exhibited by female mountain pine beetle offspring is reinforced by context-dependent maternal effects arising from parental host selection, and in situ exposure to conspecifics. These results demonstrate potential proximate mechanisms that control population dynamics in eruptive forest insects, and will allow for more accurate predictions of continued impact and spread of these species.

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Sexing live mountain pine beetles Dendroctonus ponderosae: refinement of a behavioral method for Dendroctonus spp.

Cited by 14 publications

References 27 publications

Repurposing population genetics data to discern genomic architecture: A case study of linkage cohort detection in mountain pine beetle (Dendroctonus ponderosae)

Repurposing population genetics data to discern genomic architecture: A case study of linkage cohort detection in mountain pine beetle (Dendroctonus ponderosae)

Morphological variation associated with dispersal capacity in a tree‐killing bark beetleDendroctonus ponderosaeHopkins

Breeding matters: Natal experience influences population state-dependent host acceptance by an eruptive insect herbivore

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