Sexual and apomictic plant reproduction in the genomics era: exploring the mechanisms potentially useful in crop plants

Dwivedi, Sangam L.; Perotti, Enrico; Upadhyaya, Hari D.; Ortíz, Rodomiro

doi:10.1007/s00497-010-0144-x

Cited by 39 publications

(8 citation statements)

References 102 publications

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“…apple, citrus, mango and strawberries (Dwivedi et al, 2010). Transferring apomixis to major crops worldwide would produce a huge impact on agriculture since it would enable the establishment of hybrid combinations, and their maintenance and propagation by seed (Vielle-Calzada et al, 1996;Dwivedi et al, 2010). The prospect of cloning genotypes has great potential due to increasing commercialization of hybrids in different crops and the enormous cost that represents the annual generation of hybrid seed.…”

Section: Discussionmentioning

confidence: 99%

Transcriptome analysis of seed development in apomictic Paspalum notatum

Felitti

Acuña

Ortiz

et al. 2015

Annals of Applied Biology

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The seed developmental process involves various tissues with several ploidy levels and different genetic origins. Therefore, its characterisation at the transcriptome level is certainly a challenge. The hypothesis of endosperm balance number (EBN) postulates that each species has an effective number that is important for normal endosperm and seed development to occur. Understanding endosperm formation in apomictic plants is crucial for the perspective of transferring apomixis to sexual species of agronomic interest. Since sexual tetraploid Paspalum plants fit the EBN premise, the EBN insensitivity observed in apomictic plants might be a requirement for the spread of pseudogamous apomixis. Crosses using several cytotypes of Paspalum notatum were made in order to induce the development of seeds with different maternal/paternal genomic ratios in the endosperm. A transcriptome characterisation of ovaries 3 h after pollination was performed using cDNA-AFLP methodology. Forty-six of the 100 differentially expressed transcript-derived fragments (DETDFs) were specifically found in crosses in which apomictic plants were used as the female parent and presented a predicted m : p ratio in the endosperm that was different to the 2:1 requirement of the EBN. Moreover, 12 of the DETDFs presented identity with proteins that were differentially expressed in response to changes in the levels of extracellular ATP (eATP) in Arabidopsis cell suspension cultures. eATP is an important molecular switch in plants that tightly controls organellar energy metabolism and activates gene expression controlling specific growth and developmental programmes. The results suggest that eATP-mediated signalling could be involved in the regulation of endosperm development.

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Section: Discussionmentioning

confidence: 99%

Transcriptome analysis of seed development in apomictic Paspalum notatum

Felitti

Acuña

Ortiz

et al. 2015

Annals of Applied Biology

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“…Promising results have already been obtained in arabidopsis; a combination of maternal hypomethylation with loss of fie function led to the development of endosperm without fertilization (Vinkenoog et al, 2000). To date, several promoters have been described that are active in the ovule or the gametophyte (Yu et al, 2005;Nain et al, 2008;Dwivedi et al, 2010). However, the choice of the promoter for transformation strategies to produce an artificial apomict would depend on the temporal and spatial expression patterns of the particular candidate gene targeted, and therefore should be selected on an individual basis.…”

Section: Gene Transfer As a Definitive Tool To Validate Candidate Apomentioning

confidence: 99%

Harnessing apomictic reproduction in grasses: what we have learned from Paspalum

et al. 2013

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In this article, the available literature characterizing apomixis in Paspalum spp. and its use in breeding is critically reviewed. In particular, a comparison is made across species of the structure and function of the genomic region controlling apomixis in order to identify a common core region shared by all apomictic Paspalum species and where apomixis genes are likely to be localized. Candidate genes are discussed, either as possible genetic determinants (including homologs to signal transduction and RNA methylation genes) or as downstream factors (such as cell-to-cell signalling and auxin response genes) depending, respectively, on their co-segregation with apomixis or less. Strategies to validate the role of candidate genes in apomictic process are also discussed, with special emphasis on plant transformation in natural apomictic species.

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“…Because meiosis is either completely bypassed (apospory) or extremely altered (diplospory) in apomixis, genes related to female sporogenesis are thought to be more specifically involved in diplosporic apomixis, and genes involved in embryo sac cell identity are presumably crucial for aposporic apomixis. Overall, genes associated with female gametogenesis and egg cell development are likely shared between sexual and apomictic pathways (for details on these aspects see reviews by Tucker et al 2003; Koltunow and Grossniklaus 2003; Ozias-Akins and van Dijk 2007; Tucker and Koltunow 2009; Albertini et al 2010; Dwivedi et al 2010). …”

Section: Functional Analysis Of Genes Miming Apomixis In Sexual Modelmentioning

confidence: 99%

Apomixis in plant reproduction: a novel perspective on an old dilemma

2013

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Seed is one of the key factors of crop productivity. Therefore, a comprehension of the mechanisms underlying seed formation in cultivated plants is crucial for the quantitative and qualitative progress of agricultural production. In angiosperms, two pathways of reproduction through seed exist: sexual or amphimictic, and asexual or apomictic; the former is largely exploited by seed companies for breeding new varieties, whereas the latter is receiving continuously increasing attention from both scientific and industrial sectors in basic research projects. If apomixis is engineered into sexual crops in a controlled manner, its impact on agriculture will be broad and profound. In fact, apomixis will allow clonal seed production and thus enable efficient and consistent yields of high-quality seeds, fruits, and vegetables at lower costs. The development of apomixis technology is expected to have a revolutionary impact on agricultural and food production by reducing cost and breeding time, and avoiding the complications that are typical of sexual reproduction (e.g., incompatibility barriers) and vegetative propagation (e.g., viral transfer). However, the development of apomixis technology in agriculture requires a deeper knowledge of the mechanisms that regulate reproductive development in plants. This knowledge is a necessary prerequisite to understanding the genetic control of the apomictic process and its deviations from the sexual process. Our molecular understanding of apomixis will be greatly advanced when genes that are specifically or differentially expressed during embryo and embryo sac formation are discovered. In our review, we report the main findings on this subject by examining two approaches: i) analysis of the apomictic process in natural apomictic species to search for genes controlling apomixis and ii) analysis of gene mutations resembling apomixis or its components in species that normally reproduce sexually. In fact, our opinion is that a novel perspective on this old dilemma pertaining to the molecular control of apomixis can emerge from a cross-check among candidate genes in natural apomicts and a high-throughput analysis of sexual mutants.

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Sexual and apomictic plant reproduction in the genomics era: exploring the mechanisms potentially useful in crop plants

Cited by 39 publications

References 102 publications

Transcriptome analysis of seed development in apomictic Paspalum notatum

Transcriptome analysis of seed development in apomictic Paspalum notatum

Harnessing apomictic reproduction in grasses: what we have learned from Paspalum

Apomixis in plant reproduction: a novel perspective on an old dilemma

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