Sexual differences in Salmon gonadotropin

Abstract

Summary. Salmon (Oncorhynchus tschawytscha) GTH's were purified from a mixture of pituitaries of both sexes or from separate male and female glands. Some of their biochemical, immunological and biological properties have been compared. These hormones differed essentially in amino acid composition, especially lysine, histidine and half cystine residues, and by the presence of a-amino (or a-aminoiso) butyric acid in male and GTH 11 preparations. The same differences were found between GTH 1-11 and GTH male-female. Observed biochemical differences were sex-linked ; they did not influence immunological and qualitative biological effects, but could determine the affinity of the GTH of each sex or its specific receptors.Introduction.

Isolation and culture of somatotrophs from the pituitary of the rainbow trout: Immunological and physiological characterization

“…The medium was collected, then frozen, either pure or diluted, in hormone assay buffer containing 1% BSA for later GtH2 and GH determination using specific radioimmunoassays (GtH2: Breton et al, 1978;GH: Le Bail et al, 1991).…”

Presence of specific growth hormone binding sites in rainbow trout (Oncorhynchus mykiss) tissues: Characterization of the hepatic receptor

“…Sea bream (Sparus aurata) GH (dGH) (Pagelson, Zohar, and Le Bail, unpublished data) and chinook GtH (sGtH) (Breton et al, 1978), and PRL (sPRL) (Prunet and Houdebine, 1984) were purified in our laboratory. Ovine GH (oGH) and FSH (oFSH) and bovine GH (bGH) and TSH (bTSH) were obtained from the National Institute of Arthritis and Metabolic Diseases, National Institute of Health (Bethesda, MD).…”

Ultrastructural studies on experimentally induced vitellogenesis in juvenile rainbow trout (Salmo gairdneri R.)

“…S G ]mon-gonadotropin was purified from male and female Onchorynchus tshawytscha pituitaries. The purification procedure for S-GTH has been described elsewhere (Breton, Prunet and Reinaud, 1978 (fig. 2).…”