Sexual dimorphism, but not testosterone itself, is responsible for ankylosing enthesitis of the ankle in B10.BR (H-2k) male mice

Capková, J; Iványi, P; Rehakova, Zuzana

doi:10.1136/ard.2005.039800

Cited by 6 publications

(4 citation statements)

References 12 publications

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“…Males are also exclusively affected by Ankylosing Enthesopathy (ANKENT), a spontaneous joint disease that begins with development of inflammatory cells at enthesis sites, followed by proliferation of cartilage and connective tissue, and results in or causes ankyloses of the tarsal joint [ 2 , 10 , 11 ]. Similar to the C57BL/6J mice affected in this study, ANKENT occurs in group housed male mice on a C57BL background (most notably the B10.BR strain), and does not develop in singly housed males, breeder males, or females of a similar age range [ 12 , 13 ]. While OA and ANKENT are two different musculoskeletal disease processes, they are both sexually dimorphic in which similar strains and ages of female mice do not develop these disease, however, it has been noted in both diseases that there is no linkage sex hormones [ 12 ].…”

Section: Discussionmentioning

confidence: 90%

“…Similar to the C57BL/6J mice affected in this study, ANKENT occurs in group housed male mice on a C57BL background (most notably the B10.BR strain), and does not develop in singly housed males, breeder males, or females of a similar age range [ 12 , 13 ]. While OA and ANKENT are two different musculoskeletal disease processes, they are both sexually dimorphic in which similar strains and ages of female mice do not develop these disease, however, it has been noted in both diseases that there is no linkage sex hormones [ 12 ]. Group housed males are known to have higher bone mineral density and content, which may be due to their incidence of fighting being 1.3 times higher than group housed females [ 14 , 15 ].…”

Section: Discussionmentioning

confidence: 90%

“…While the exact cause for this tarsal abnormality remains unknown, it is likely to have a multifactorial cause, similar to ANKENT, in which a combination of genetics, housing condition, and microbial flora all play a role in the disease onset and progression [10][11][12][13]16]. An advanced understanding of how tarsal abnormalities affect various behavioral/phenotypic tests will help other institutions or research groups identify this condition and understand that this disease may result in research variables.…”

Section: Plos Onementioning

confidence: 99%

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Evaluation of tarsal injuries in C57BL/6J male mice

Kick,

Anderson,

Doty

et al. 2023

PLoS ONE

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Tarsal joint abnormalities have been observed in aged male mice on a C57BL background. This joint disease consists of calcaneal displacement, inflammation, and proliferation of cartilage and connective tissue, that can progress to ankylosis of the joint. While tarsal pathology has been described previously in C57BL/6N substrains, as well as in STR/ort and B10.BR strain, no current literature describes this disease occurring in C57BL/6J mice. More importantly the behavioral features that may result from such a change to the joint have yet to be evaluated. This condition was observed in older male mice of the C57BL/6J lineage, around the age of 20 weeks or older, at a frequency of 1% of the population. To assess potential phenotypic sequela, this study sought to evaluate body weight, frailty assessment, home cage wheel running, dynamic weight bearing, and mechanical allodynia with and without the presence of pain relief with morphine. Overall mice with tarsal injuries had significantly higher frailty scores (p< 0.05) and weighed less (p<0.01) compared to unaffected mice. Affected mice had greater overall touch sensitivity (p<0.05) and they placed more weight on their forelimbs (p<0.01) compared to their hind limbs. Lastly, when housed with a running wheel, affected mice ran for a shorter length of time (p<0.01) but tended to run a greater distance within the time they did run (p<0.01) compared to unaffected mice. When tested just after being given morphine, the affected mice performed more similarly to unaffected mice, suggesting there is a pain sensation to this disease process. This highlights the importance of further characterizing inbred mouse mutations, as they may impact research programs or specific study goals.

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Section: Discussionmentioning

confidence: 90%

Section: Discussionmentioning

confidence: 90%

Section: Plos Onementioning

confidence: 99%

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Evaluation of tarsal injuries in C57BL/6J male mice

Kick,

Anderson,

Doty

et al. 2023

PLoS ONE

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“…Analogously to human spondyloarthropathies (SpA) [ 7 , 8 ], enthesitis is a specific marker of the affected joints and the disease was classified as ank ylosing ent hesopathy (ANKENT) [ 9 ]. The disease occurs spontaneously in some inbred mouse strains [ 10 , 11 ] and almost exclusively in males [ 12 ]. Its incidence is significantly increased in HLA-B27 mice [ 11 ], confirming an implication of the B27 allele in ANKENT development.…”

Section: Introductionmentioning

confidence: 99%

Lipopolysaccharide treatment suppresses spontaneously developing ankylosing enthesopathy in B10.BR male mice: The potential role of interleukin-10

Capková

Hrncírová

Kubátová

et al. 2012

BMC Musculoskelet Disord

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Background: Ankylosing enthesopathy (ANKENT) is an animal model of human ankylosing spondylitis. ANKENT is an inflammatory disease affecting the ankle and tarsal joints of the hind limbs in susceptible mouse strains. In the disease, the participation of intestinal microbiota components was suggested. Therefore, we attempted to increase the incidence of ANKENT by systemic administration of lipopolysaccharide (LPS), which is a component of bacterial cellular walls and stimulates inflammatory processes. Methods: ANKENT occurrence, serum cytokine profiles, spleen cellular composition and in vitro cytokine response to LPS were analysed in LPS-treated and control LPS-untreated B10.BR male mice. Results: Contrary to expectations, LPS treatment decreased the incidence of ANKENT in LPS-treated group compared to control LPS-untreated group. Flow cytometry analysis of splenocytes showed an increased percentage of macrophages, dendritic cells and neutrophils and a decreased percentage of B cells, T cells and T helper cells in LPS-treated males following LPS administration. In addition, LPS-treated males had significantly elevated IL-6 and IL-10 serum levels. At 20-22 weeks after the final LPS application, splenocytes from LPS-treated mice were more susceptible to in vitro LPS stimulation than those of the controls and produced significantly higher levels of TNFα and IL-6.

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Animal Models of Spondyloarthritis

Taurog

2009

Advances in Experimental Medicine and Biology

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Sexual dimorphism, but not testosterone itself, is responsible for ankylosing enthesitis of the ankle in B10.BR (H-2k) male mice

Cited by 6 publications

References 12 publications

Evaluation of tarsal injuries in C57BL/6J male mice

Evaluation of tarsal injuries in C57BL/6J male mice

Lipopolysaccharide treatment suppresses spontaneously developing ankylosing enthesopathy in B10.BR male mice: The potential role of interleukin-10

Animal Models of Spondyloarthritis

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