Sexual transmission of T-DNA in abnormal tobacco regenerants transformed by octopine and nopaline strains of Agrobacterium tumefaciens

Wöstemeyer, Anke; Otten, Léon; Schell, J.

doi:10.1007/bf00425565

Cited by 15 publications

(7 citation statements)

References 49 publications

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“…In experiments using direct DNA transformation or Agrobacterium tumefaciens to transfer DNA to tobacco, a single genetic locus has been found in 18/20 cases (Otten et al 1981 ;Memelink et al 1983;Wostemeyer et al 1984;Paszkowski et al 1984;De Block et al 1984;Horsch et al 1984Horsch et al , 1985Sengupta-Gopalan etal. 1985;Hain et al 1985;Potrykus et al 1985;Deshayes et al 1985;Peerbolte et al 1985;de Framond et al 1986;Czako and Marton 1986).…”

Section: Discussionmentioning

confidence: 96%

T-DNA structure in transgenic tobacco plants with multiple independent integration sites

Spielmann¹,

Simpson²

1986

Molec. Gen. Genet.

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Summary. Transgenic tobacco plants were produced by inoculation of leaf disks with Agrobacterium tumefaciens harboring a disarmed binary vector containing soybean leghemoglobin Lbc3 and glycinin G2 genes. Physical and genetic characterization of these plants indicated that one to six copies of DNA from the vector were transferred and maintained in the plant genome. Approximately 30% of the copies transferred were found to be incomplete or rearranged and in some cases joined as inverted repeats. The transferred DNA was found at multiple genetic loci in five of the six cases examined. In one plant, kanamycin-resistance traits were at four independent chromosomal positions, although two were genetically linked at about 3 centimorgans. Thus, Agrobacterium-mediated DNA transfer to plants has some characteristics in common with "natural" systems in animals, such as retroviral or P-element derived systems, some characteristics in common with "artificial" systems, such as microinjection, electroporation, or calcium phosphate coprecipitation techniques, and some novel characteristics.

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Section: Discussionmentioning

confidence: 96%

T-DNA structure in transgenic tobacco plants with multiple independent integration sites

Spielmann¹,

Simpson²

1986

Molec. Gen. Genet.

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“…Early work with the wild-type auxin and cytokinin genes of the T-DNA of Agrobacterium tumefaciens showed that the expression of these genes strongly interfered with various aspects of normal plant development [37,38].…”

Section: Introductionmentioning

confidence: 99%

IAA synthesis and root induction with iaa genes under heat shock promoter control

et al. 1990

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We have devised a heat shock-inducible indole-3-acetic acid (IAA) synthesis system for plant cells, which is based on the iaa genes of the Agrobacterium tumefaciens T-DNA and the heat shock promoter hsp70 of Drosophila melanogaster. Two DNA constructs were tested: one contains the iaaM gene linked to the hsp70 promoter (hsp70-iaaM) and encodes the production of indoleacetamide (IAM), the other contains hsp70-iaaM and the wild-type iaaH gene which codes for the conversion of IAM into IAA (hsp70-iaaM/iaaH). Heat shock-controlled IAM and IAA synthesis was tested on two levels: biochemically by measuring IAM and IAA levels in Kalanchoe stem segments infected with the two constructs, and morphologically by IAA-dependent root formation on Kalanchoe plants, on carrot discs and on tobacco leaf fragments. At both levels the responses were found to be controlled by the heat shock promoter. IAM levels of segments infected with hsp70-iaaM increased 6-fold upon heat shock induction to 240 pmol IAM per stem segment. The accumulation of IAA in segments infected with hsp70-iaaM/iaaH and heat-shocked was found to be more variable, possibly due to IAA transport and metabolism. Heat shock treatment of Kalanchoe plants and tobacco leaf fragments infected with hsp70-iaaM/iaaH led to a strong increase in root formation. On carrot discs, heat shock-specific root induction was also demonstrated, but the responses differed between individual carrots.

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“…Usually they did not form roots and had a distinct morphology which reverted to normal following grafting (34,39). The plants formed flowers and T-DNA was transmitted through meiosis (20,28,38,39).…”

Section: Introductionmentioning

confidence: 99%

T-DNA genes to study plant development: precocious tuberisation and enhanced cytokinins in A. tumefaciens transformed potato

Ooms

Lenton

1985

Plant Mol Biol

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Potato Line Mb1501B is a derivative of the cultivar Maris Bard (Solanum tuberosum), transformed with T-DNA from A. tumefaciens strain LBA1501. In culture it grew as frequently branching stunted shoots with a basal callus, lacking roots. These shoots did not form tubers. When grafted, Mb1501B shoots gradually became morphologically more normal and aerial tubers formed readily. Cultured Mb1501B shoots contained 100-200-fold higher concentrations of the biologically-active cytokinins zeatin, zeatin riboside and their corresponding side-chain o-glucosides than untransformed Maris Bard shoots. Cultured Mb1501B shoots contained approximately a 3-fold lower concentration of indole acetic acid (IAA). In grafted Mb1501B plants a 3-10-fold higher concentration of the active cytokinins was found compared with untransformed plants and no difference in IAA concentration.

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Sexual transmission of T-DNA in abnormal tobacco regenerants transformed by octopine and nopaline strains of Agrobacterium tumefaciens

Cited by 15 publications

References 49 publications

T-DNA structure in transgenic tobacco plants with multiple independent integration sites

T-DNA structure in transgenic tobacco plants with multiple independent integration sites

IAA synthesis and root induction with iaa genes under heat shock promoter control

T-DNA genes to study plant development: precocious tuberisation and enhanced cytokinins in A. tumefaciens transformed potato

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