Sexually dimorphic expression of steroidogenic factor 1 (SF-1) in developing gonads of the American bullfrog, Rana catesbeiana

Mayer, Loretta P.; Overstreet, Stefanie L; Dyer, Cheryl A.; Propper, Catherine R.

doi:10.1016/s0016-6480(02)00019-9

Cited by 32 publications

(35 citation statements)

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“…Testes from 3-and 10-month-old male carp show higher SF-1 expression levels than those of female ovaries. Sex differences of SF-1 mRNA expression were found at the early and middle stages of gonadal development, similar to the results found in rat fetuses (Hatano et al, 1994) and mature frogs (Mayer et al, 2002). This indicates that SF-1 may participate in the development of carp testes.…”

Section: Discussionsupporting

confidence: 85%

Developmental expression of steroidogenic factor-1, cyp19a1a and cyp19a1b from common carp (Cyprinus carpio)

Tang

Hao

et al. 2010

General and Comparative Endocrinology

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Section: Discussionsupporting

confidence: 85%

Developmental expression of steroidogenic factor-1, cyp19a1a and cyp19a1b from common carp (Cyprinus carpio)

Tang

Hao

et al. 2010

General and Comparative Endocrinology

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“…Expression of Dmrt1 has been observed in the frog testis, suggesting that, as in all other vertebrates, it also participates in testicular differentiation in amphibians [36]. Expression of the SF1 protein was shown to be higher in the ovaries than in the testis at the time of sexual differentiation [37].…”

Section: Genes Involved In Gonadal Sex Differentiationmentioning

confidence: 89%

Sex determination: the amphibian models

2004

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-We review and discuss current knowledge about sex determination in amphibians. The astonishing wide variety of mechanisms of genotypic sex determination is presented and discussed in an evolutionary context. We recall the natural occurrence of transitory juvenile hermaphroditism in some species. Our present knowledge of the mechanisms of sex determination in amphibians is compared to that in mammals. The influence of epigenetic factors, and especially temperature is highlighted. In amphibians, the influence of temperature on sexual differentiation, that can prevail over genotypic sex determination, remains poorly considered in publications. We suggest that studies on genetic and epigenetic factors of gonadal sex differentiation in amphibians could provide substantial information on the evolutionary process of sex determination mechanisms in current living vertebrates.sex determination mechanisms / sexual differentiation / hermaphroditism / epigenetic factors

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“…We previously observed almost complete sexual differentiation of the gonads in both sexes in bullfrogs by Gosner stage 35 (13,16). Here we examined tissues from stages 32-36 for sexually distinct gonadal morphology (Figure 1).…”

Section: Resultsmentioning

confidence: 90%

“…Using bullfrog tadpoles, we found a sexually dimorphic pattern of SF-1 protein expression that emerges at the time of gonadal sexual differentiation (16). In females, the expression of SF-1 increases just after the formation of ovaries, and in males SF-1 expression decreases as the testes develop.…”

mentioning

confidence: 89%

“…To quantitate SF-1 protein expression in the developing frog gonads, we first determined the minimum amount of protein necessary to detect SF-1 in the smallest gonadal sample (16). Densitometric analysis for each film image of the individual immunoblots was performed using the Eagle Eye II computerized digital camera and Eagle Sight 3.0 software (Stratagene, La Jolla, CA).…”

Section: Articles | Mayer Et Almentioning

confidence: 99%

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Exposure to 4-tert-octylphenol accelerates sexual differentiation and disrupts expression of steroidogenic factor 1 in developing bullfrogs.

Mayer

Dyer

Propper

2003

Environ Health Perspect

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Sex-specific gonadal steroidogenesis during development is critical to differentiation of the sexually dimorphic phenotype and reproductive function of adult organisms. Environmental contaminants may affect the process of sexual differentiation through disruption of steroid production and/or action. Control of the steroidogenic metabolic pathway is regulated partly by P450 cytochrome hydroxylases, and the expression of many of these enzymes is controlled by the orphan nuclear receptor, steroidogenic factor-1 (SF-1). In mammals, SF-1 expression is critical for development of the reproductive axis and adult reproductive function. In the bullfrog Rana catesbeiana, during sequential stages of development encompassing sexual differentiation, SF-1 protein expression becomes elevated in ovaries of sexually differentiating females, whereas expression in testes decreases. We exposed tadpoles to the industrial pollutant octylphenol (OP) for 24 hr before and during the critical stages of sexual differentiation to determine whether this known endocrine disruptor affects sex differentiation and SF-1 expression. We found that both females and males treated with an environmentally relevant low dose (10(-9)M) of OP underwent early gonadal differentiation. Furthermore, OP exposure disrupted the sexually dimorphic expression of SF-1 that occurs during sexual differentiation. Our results suggest that OP exposure may affect developmental processes that could ultimately influence adult reproductive function and that these disruptive effects may be mediated in partly through disturbances in gene regulation by SF-1.

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Sexually dimorphic expression of steroidogenic factor 1 (SF-1) in developing gonads of the American bullfrog, Rana catesbeiana

Cited by 32 publications

References 38 publications

Developmental expression of steroidogenic factor-1, cyp19a1a and cyp19a1b from common carp (Cyprinus carpio)

Developmental expression of steroidogenic factor-1, cyp19a1a and cyp19a1b from common carp (Cyprinus carpio)

Sex determination: the amphibian models

Exposure to 4-tert-octylphenol accelerates sexual differentiation and disrupts expression of steroidogenic factor 1 in developing bullfrogs.

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