Sexually-dimorphic expression of tyrosine hydroxylase immunoreactivity in the brain of a vocal teleost fish (Porichthys notatus)

Goebrecht, Geraldine K.E.; Kowtoniuk, Robert A.; Kelly, Brenda G.; Kittelberger, J. Matthew

doi:10.1016/j.jchemneu.2014.01.001

“…The overall distribution of TH-ir in the midshipman CNS is largely consistent with an earlier preliminary report of general TH-ir distribution in midshipman brain using a different antibody and non-fluorescent detection (Bass et al, 2001b), and a recent report using the same antibody as the present study with non-fluorescent detection to describe TH-ir distribution patterns (Goebrecht et al, 2014). Thus, while the present study is not the first to report TH-ir in association with several known vocal and auditory centers in the midshipman CNS, we utilized double-labeling experiments which combined TH immunofluorescence with transneuronal backfill labeling of auditory circuitry and the vocal pattern generator to demonstrate putative TH-ir terminals on individual cells comprising both the peripheral and central auditory system as well as the octavolateralis efferent nucleus and vocal motor system.…”

Section: Discussionsupporting

confidence: 91%

“…On Western blots it does not react with dopamine-betahydroxylase, phenylalanine hydroxylase, trytophan hydroxylase, dehydropteridine reductase, sepiapterin reductase or phenethanolamine-N-methyl transferase (manufacturer's data). Western blot analysis of rat and fish brain extracts, including midshipman, show similar expected bands of 59-63kDa (Adrio et al, 2011;Carrera et al, 2012;Gayoso et al, 2011;Goebrecht et al, 2014;manufacturer's data). This antibody stains the appropriate pattern (i.e., known CA populations of neurons and projection patterns) in all brain regions as previously documented in midshipman and other fish species (Carrera et al, 2012;Goebrecht et al, 2014;Kuscha et al, 2012;McLean and Fetcho, 2004a) and other vertebrates (e.g., Hayes et al, 2011;Nakano et al, 2009).…”

Section: Antibody Characterizationmentioning

confidence: 80%

Catecholaminergic connectivity to the inner ear, central auditory, and vocal motor circuitry in the plainfin midshipman fish porichthys notatus

Forlano

¹

,

Kim

²

,

Krzyminska

³

et al. 2014

J of Comparative Neurology

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Although the neuroanatomical distribution of catecholaminergic (CA) neurons has been well documented across all vertebrate classes, few studies have examined CA connectivity to physiologically and anatomically identified neural circuitry that controls behavior. The goal of this study was to characterize CA distribution in the brain and inner ear of the plainfin midshipman fish (Porichthys notatus) with particular emphasis on their relationship with anatomically labeled circuitry that both produces and encodes social acoustic signals in this species. Neurobiotin labeling of the main auditory endorgan, the saccule, combined with tyrosine hydroxylase immunofluorescence (TH-ir) revealed a strong CA innervation of both the peripheral and central auditory system. Diencephalic TH-ir neurons in the periventricular posterior tuberculum, known to be dopaminergic, send ascending projections to the ventral telencephalon and prominent descending projections to vocal-acoustic integration sites, notably the hindbrain octavolateralis efferent nucleus, as well as onto the base of hair cells in the saccule via nerve VIII. Neurobiotin backfills of the vocal nerve in combination with TH-ir revealed CA terminals on all components of the vocal pattern generator which appears to largely originate from local TH-ir neurons but may include diencephalic projections as well. This study provides strong evidence for catecholamines

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“…Previously, dopaminergic cell bodies were observed near the POA in several teleost fish species, including A. burtoni (Goebrecht et al, 2014;O'Connell et al, 2010;Saha et al, 2015). We tested whether dopaminergic processes are closely apposed to GnRH1 neurons.…”

Section: Resultsmentioning

confidence: 98%

“…Previous studies demonstrated sexually dimorphic regulation of dopaminergic signaling in the hypothalamus and pituitary of both mammals and fish (Goebrecht et al, 2014;Saha et al, 2015;Scott et al, 2015). Although androgenic steroids regulate dopamine receptor expression in the fish POA (Pasqualini et al, 2009), there are, to our knowledge, no studies testing for sex-specific differences in dopamine receptor-mediated inhibition of POA neural activity.…”

Section: D2r-mediated Inhibition Of Gnrh1 Neurons Is Not Sexually Dimmentioning

confidence: 96%

“…These results show that the inhibitory mechanisms postsynaptic to dopamine release onto POA GnRH1 neurons are not sexually dimorphic. However, presynaptic dopamine production or release could vary by sex; sexually dimorphic TH expression, as has been observed in fish and mammals (Saha et al, 2015;Scott et al, 2015), could result in sexually dimorphic regulation of dopaminergic control of reproductive behaviors (Goebrecht et al, 2014).…”

Section: D2r-mediated Inhibition Of Gnrh1 Neurons Is Not Sexually Dimmentioning

confidence: 99%

See 1 more Smart Citation

Dopaminergic inhibition of gonadotropin-releasing hormone neurons in the cichlid fish,Astatotilapia burtoni

Bryant

¹

,

Greenwood

²

,

Juntti

³

et al. 2016

Journal of Experimental Biology

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Dopamine regulates reproduction in part by modulating neuronal activity within the hypothalamic-pituitary-gonadal (HPG) axis. Previous studies suggested numerous mechanisms by which dopamine exerts inhibitory control over the HPG axis, ultimately changing the levels of sex steroids that regulate reproductive behaviors. However, it is not known whether these mechanisms are conserved across vertebrate species. In particular, it is unknown whether mechanisms underlying dopaminergic control of reproduction are shared between mammals and teleost fish. In mammals, dopamine directly inhibits gonadotropin-releasing hormone (GnRH1) hypothalamic neurons, the gatekeepers for activation of the HPG axis. Here, we demonstrate, for the first time in teleost fish, dopaminergic control of GnRH1 neurons via direct dopamine type-2-like receptor (D2R)-mediated inhibition within the hypothalamus. These results suggest that direct dopaminergic control of GnRH1 neurons via interactions in the hypothalamus is not exclusive to tetrapod reproductive control, but is likely conserved across vertebrate species.

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Catecholaminergic connectivity to the inner ear, central auditory, and vocal motor circuitry in the plainfin midshipman fish porichthys notatus

Forlano

¹

,

Kim

²

,

Krzyminska

³

et al. 2014

J of Comparative Neurology

View full text Add to dashboard Cite

Although the neuroanatomical distribution of catecholaminergic (CA) neurons has been well documented across all vertebrate classes, few studies have examined CA connectivity to physiologically and anatomically identified neural circuitry that controls behavior. The goal of this study was to characterize CA distribution in the brain and inner ear of the plainfin midshipman fish (Porichthys notatus) with particular emphasis on their relationship with anatomically labeled circuitry that both produces and encodes social acoustic signals in this species. Neurobiotin labeling of the main auditory endorgan, the saccule, combined with tyrosine hydroxylase immunofluorescence (TH-ir) revealed a strong CA innervation of both the peripheral and central auditory system. Diencephalic TH-ir neurons in the periventricular posterior tuberculum, known to be dopaminergic, send ascending projections to the ventral telencephalon and prominent descending projections to vocal-acoustic integration sites, notably the hindbrain octavolateralis efferent nucleus, as well as onto the base of hair cells in the saccule via nerve VIII. Neurobiotin backfills of the vocal nerve in combination with TH-ir revealed CA terminals on all components of the vocal pattern generator which appears to largely originate from local TH-ir neurons but may include diencephalic projections as well. This study provides strong evidence for catecholamines as important neuromodulators of both auditory and vocal circuitry and acoustic-driven social behavior in midshipman fish. This first demonstration of TH-ir terminals in the main endorgan of hearing in a non-mammalian vertebrate suggests a conserved and important anatomical and functional role for dopamine in normal audition.

show abstract

Sexually-dimorphic expression of tyrosine hydroxylase immunoreactivity in the brain of a vocal teleost fish (Porichthys notatus)

Cited by 23 publications

References 162 publications

Catecholaminergic connectivity to the inner ear, central auditory, and vocal motor circuitry in the plainfin midshipman fish porichthys notatus

Catecholaminergic connectivity to the inner ear, central auditory, and vocal motor circuitry in the plainfin midshipman fish porichthys notatus

Dopaminergic inhibition of gonadotropin-releasing hormone neurons in the cichlid fish,Astatotilapia burtoni

Catecholaminergic connectivity to the inner ear, central auditory, and vocal motor circuitry in the plainfin midshipman fish porichthys notatus

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