Sexualwissenschaft und gesundheitswissenschaften

Haeberle, Erwin J.

doi:10.1007/bf02956924

Cited by 2 publications

(2 citation statements)

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“…One possible explanation for the impaired motion of I341A actin filaments is that a mechanical load inhibits their sliding. It has been previously demonstrated that under the standard conditions of the in Vitro motility assay [Materials and Methods; see also Kron et al (1991)] an internal mechanical load can develop, probably as a result of photodamage to myosins (Haeberle, 1994(Haeberle, , 1995. We tested for the presence of mechanical load in our system by inclusion of 2 mM ADP in the assay buffer.…”

Section: Construction Of Mutationsmentioning

confidence: 99%

Mutational Analysis of the Role of Hydrophobic Residues in the 338−348 Helix on Actin in Actomyosin Interactions

et al. 1996

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Yeast actin mutants with alanines replacing I341 and I345 were studied to assess the role of hydrophobic residues in the alpha-helix 338-348 in interactions with myosin. In structural models of the actomyosin complex, this helix on actin was assigned a prominent role in the strong binding of myosin to actin. Substitution of I341 with alanine reduced the strong binding of actin to myosin subfragment-1 (S1) 9-fold compared to wild-type actin. In addition, the Vmax of the actin-activated S1 ATPase was reduced 4-fold with no change in the Km. In contrast, substitution of I345 with alanine had no significant effect on either the strong binding to S1 or the actin activation of S1 ATPase. The I341A actin filaments were found to slide in the in vitro motility assays at a lower mean velocity (1.6 +/- 0.4 microns/s) than wild-type actin filaments (2.6 +/- 0.3 microns/s). Only 65% of the mutant actin filaments moved in such assays in comparison to 95% of the wild-type filaments. However, addition of 2.0 mM MgADP to the motility assay buffer induced movement of all the I341A filaments at a velocity (1.6 +/- 0.1 microns/s) similar to that of wild-type actin (1.7 +/- 0.1 microns/s). The decrease in motility of the I341A actin filaments in the absence of ADP was attributed to a negative load slowing the mutant filaments and the smaller force produced by the heavy meromyosin and I341A actin system. The latter conclusion was confirmed by showing that a greater percentage of NEM-modified heavy meromyosin (external load) was required for arresting the motion of wild-type actin in the in vitro motility assay than that needed for stopping the I341A filaments.

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Section: Construction Of Mutationsmentioning

confidence: 99%

Mutational Analysis of the Role of Hydrophobic Residues in the 338−348 Helix on Actin in Actomyosin Interactions

et al. 1996

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“…There is no doubt that modern European sexual science indeed represented a very specific way of knowing about sex, and numerous historiographic attempts to trace its emergence have converged on Western Europe in the long 19th century as a pivotal context in the formation of sexology's unique approach to sexual perversions, pathologies, and types of people (Bauer, 2015;Beccalossi, 2012;Bland and Doan, 1998;Chaperon, 2007;Crozier and Bonis, 2001;Cryle and Downing, 2010;Cryle and Moore, 2011;Davidson, 2001;Flore, 2020;Foucault, 1976;Gallagher and Laqueur, 1987;Leck, 2016;McLaren, 1997;Moore, 2016;Rosario, 1997;Sigusch, 2008). In broad surveys of its history, sexual science and medicine is generally described as a historically novel genre that first appeared in French, German/Austrian, Italian, and English contexts between c.1750 and c.1880, proliferating to numerous other cultural contexts between c.1880 and c.1930, then to Asian, Middle Eastern, South American, Australasian, and African cultures over the course of the 20th century (Bullough, 1997;Haeberle, 1995Haeberle, , 1997Hekma, 1989;Nye, 1991). However, there has also been an important scholarly movement to consider more multidirectional global entanglements in the modern history of ideas about sexuality and gender generally, particularly those relating to Europe and the Middle East (El Shakry, 2020: 63-81;Leck, 2017;Massad, 2007;Najmabadi, 2005Najmabadi, , 2014Newman, 2014: 49-56;Surkis, 2019;Ze'evi, 2006).…”

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confidence: 99%

Modern European sexological and orientalist assimilations of medieval Islamicate ‘ilm al-bah to erotology

Moore

2021

History of the Human Sciences

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This article discusses the term erotology, which was applied to medieval Islamicate ‘ilm al-bah (the science of coitus), as well as other world traditions of sexual knowledge, by European sexologists of the late 19th and early 20th centuries, who contrasted it with their own forms of inquiry into sexual matters in the modern field of sexual science. It argues that the homogenisation and minimisation of all ancient and non-European forms of medical knowledge about sex, even one as substantial as the ‘ilm al-bah tradition, supported a particular story about the origins of sexology's own emergence as a new and unprecedented biomedical and scientific way of knowing, characterised by an opposition assumed between sexuality and religion, by a view of sexual variations as perversions or pathologies, and by a view of Arabs and Muslims as sexually excessive. The article focusses on French, English, German, Austrian, and Italian sources of the 19th century that discussed the history of sexual medicine, relating these accounts to recent attempts to historicise sexology. It considers how forms of colonial hierarchy and exoticist views of non-European cultures impacted the dismissal of ‘ilm al-bah among European sexual scientists and how they may continue to exert an influence on forms of modern historical inquiry that are not attentive to scholarship on medieval Islamicate sexual medicine.

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Sexualwissenschaft und gesundheitswissenschaften

Cited by 2 publications

References 1 publication

Mutational Analysis of the Role of Hydrophobic Residues in the 338−348 Helix on Actin in Actomyosin Interactions

Mutational Analysis of the Role of Hydrophobic Residues in the 338−348 Helix on Actin in Actomyosin Interactions

Modern European sexological and orientalist assimilations of medieval Islamicate ‘ilm al-bah to erotology

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