SFRP1 regulates the growth of retinal ganglion cell axons through the Fz2 receptor

Rodríguez, Josana; Esteve, Pilar; Weinl, Christine; Ruiz, José María García; Fermin, Yasmín; Trousse, Françoise; Dwivedy, Asha; Holt, Christine E.; Bovolenta, Paola

doi:10.1038/nn1547

Cited by 134 publications

(140 citation statements)

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“…This possibility has been supported by the ability of sFRPs and Fz proteins to form homo-and heteromeric complexes through the CRD domain (Bafico et al 1999;Rodriguez et al 2005). These findings suggest an alternative model of how sFRPs could inhibit Wnt signaling, by forming nonfunctional complexes with Fz receptors (Fig.…”

Section: Mechanism Of Actionmentioning

confidence: 89%

Secreted and Transmembrane Wnt Inhibitors and Activators

Cruciat

Niehrs

2012

Cold Spring Harbor Perspectives in Biology

537

450

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Section: Mechanism Of Actionmentioning

confidence: 89%

Secreted and Transmembrane Wnt Inhibitors and Activators

Cruciat

Niehrs

2012

Cold Spring Harbor Perspectives in Biology

537

450

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“…Extracellular or membrane-bound attractive/repulsive cues are also critical to guide axons (Tessier-Lavigne and Goodman, 1996;Guthrie, 1997;Rodriguez et al, 2005;Dickson and Gilestro, 2006;Zhu et al, 2006). Wnts, Shh, and Robo/Slit are central in guiding commissural axons (including Atoh1 ϩ neurons), before and after crossing the floor plate (Lyuksyutova et al, 2003;Bourikas et al, 2005;Howell et al, 2007;Renier et al, 2010).…”

Section: Possible Cues That Regulate Da1-axonal Projection Patternsmentioning

confidence: 99%

Axonal Patterns and Targets of dA1 Interneurons in the Chick Hindbrain

Kohl

Hadas²,

Klar³

et al. 2012

J. Neurosci.

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Hindbrain dorsal interneurons that comprise the rhombic lip relay sensory information and coordinate motor outputs. The progenitor dA1 subgroup of interneurons, which is formed along the dorsal-most region of the caudal rhombic lip, gives rise to the cochlear and precerebellar nuclei. These centers project sensory inputs toward upper-brain regions. The fundamental role of dA1 interneurons in the assembly and function of these brainstem nuclei is well characterized. However, the precise en route axonal patterns and synaptic targets of dA1 interneurons are not clear as of yet. Novel genetic tools were used to label dA1 neurons and trace their axonal trajectories and synaptic connections at various stages of chick embryos. Using dA1-specific enhancers, two contralateral ascending axonal projection patterns were identified; one derived from rhombomeres 6 -7 that elongated in the dorsal funiculus, while the other originated from rhombomeres 2-5 and extended in the lateral funiculus. Targets of dA1 axons were followed at later stages using PiggyBac-mediated DNA transposition. dA1 axons were found to project and form synapses in the auditory nuclei and cerebellum. Investigation of mechanisms that regulate the patterns of dA1 axons revealed a fundamental role of Lim-homeodomain (HD) proteins. Switch in the expression of the specific dA1 Lim-HD proteins Lhx2/9 into Lhx1, which is typically expressed in dB1 interneurons, modified dA1 axonal patterns to project along the routes of dB1 subgroup. Together, the results of this research provided new tools and knowledge to the assembly of trajectories and connectivity of hindbrain dA1 interneurons and of molecular mechanisms that control these patterns.

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“…The assay allows assessment of guidance activity of established molecules and also testing potential guidance properties of hitherto uncharacterized molecules such as RGM, the Wnt signaling inhibitor SFRP1 or the morphogen Sonic hedgehog [26][27][28] .…”

Section: Introductionmentioning

confidence: 99%

Stripe assay to examine axonal guidance and cell migration

et al. 2007

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Stripe assays have been widely employed as in vitro test systems to study the responses of growing axons, as well as migrating cells, to established or novel guidance molecules. We provide detailed protocols for both the original and the modified version of this assay, as they allow the analysis of the 'guidance properties' of active components present in crude membrane fractions or as purified molecules. Silicon matrices are used to produce striped patterns of active molecules on a surface (referred to as 'carpet'), followed by culturing of neurons, or any other cell type, on these carpets. After 1-2 days in culture, striped outgrowth of extending neuritesindicative of guided migration of cell processes-can be observed. We also discuss potential other applications (e.g., in neuronal regeneration and development) and modifications of the assay. The preparation of 10-12 carpets takes approximately 4-5 h. INTRODUCTIONThe stripe assay was originally designed by F. Bonhoeffer and co-workers 1,2 in the late 1980s to analyze fundamental axonal guidance mechanisms governing the formation of the topographic map established in the chick retino-tectal system (for review, see ref.3). Employing this assay, the presence of a graded distribution of repulsive axon guidance cues, present predominantly in posterior tectal membranes, was discovered. Subsequently, this assay was modified, permitting the identification of ephrin-As as the key components present in posterior membranes that are responsible for the repulsive properties 4-7 .The stripe assay has since been used to uncover guidance cues and mechanisms in many principal neuronal projections, including, for example, the hippocampal, thalamic and olfactory systems [8][9][10][11] . The stripe assay was also applied to investigate neurite outgrowth and guidance behavior related to neuronal regeneration 12-15 and branch formation 16 . Furthermore, the stripe assay has proven valuable in dissecting signaling cascades initiated downstream of axonal guidance receptors activated by ligands printed in a striped pattern [12][13][14][15] . In addition, the guidance properties of membrane-tethered molecules as well as the short-range action of secreted guidance molecules (such as Slits and Semaphorins) could be mimicked in vitro by this assay 17,18 . Also stripe assay experiments were conducted to unravel potential guidance properties of extracellular matrix components 19,20 and of cell adhesion molecules of the IgG superfamily 21 .Of note, the stripe assay was employed as an in vitro assay to investigate the navigation responses of both axonal growth cones toward guidance cues and of migrating cells 4,22,23 . With respect to the latter, neural crest cells 22 and oligodendrocytes 23,24 , but also non-neuronal cells such as tumor cells, have been studied 25 .The assay allows assessment of guidance activity of established molecules and also testing potential guidance properties of hitherto uncharacterized molecules such as RGM, the Wnt signaling inhibitor SFRP1 or the morphogen Sonic h...

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SFRP1 regulates the growth of retinal ganglion cell axons through the Fz2 receptor

Cited by 134 publications

References 50 publications

Secreted and Transmembrane Wnt Inhibitors and Activators

Secreted and Transmembrane Wnt Inhibitors and Activators

Axonal Patterns and Targets of dA1 Interneurons in the Chick Hindbrain

Stripe assay to examine axonal guidance and cell migration

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