Sful, a novel<i>Asull isoschizomer</i>from<i>Streptomyces fulvissimus</i>recognizing 5′-TT/CGAA-3′

Veitinger, S; Schmitz, G.; Kaluza, Klaus; Jarsch, Michael; Braun, Veit; Keßler, Christoph

doi:10.1093/nar/18.11.3424

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Restriction Endonuclease Cutting Pattern1

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1990

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“…For example, (Roberts, 1988;Leung et al, 1989;Veitinger et al, 1990). whereas there were no sites in FP22 DNA for NcoI (CCATGG) and NaeI (GCCGGC), there were many sites for BstXI (CCAN,TGG), PJMMI (CCAN,TGG) and BgZI (GCCN,GGC).…”

Section: Restriction Endonuclease Cutting Patternmentioning

confidence: 99%

Characterization of FP22, a large streptomycete bacteriophage with DNA insensitive to cleavage by many restriction enzymes

Hahn

McHenney²,

Baltz³

1990

Journal of General Microbiology

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Bacteriophage FP22 has a very broad host range within streptomycetes and appeared to form lysogens of Streptomyces ambofaciens ATCC 15154. FP22 shared strong cross-immunity and antibody cross-reactivity with bacteriophage P23, but not with seven other streptomycete bacteriophages. FP22 particles had a head diameter of 71 nm and a tail length of 307 run. The FP22 genome was 131 kb, which is the largest bacteriophage genome reported for streptomycetes. The G + C content of the genome was 46 mol% and restriction mapping indicated that FP22 DNA had discrete ends. NaCl-and pyrophosphate-resistant deletion mutants were readily isolated and the extent of the deletions defined at least 23 kb of dispensable DNA in Qvo regions of the genome. The DNA was not cleaved by most restriction endonucleases (or isoschizomers) which have been identified in the streptomycetes, including the tetranucleotide cutter MboI (GATC).

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Section: Restriction Endonuclease Cutting Patternmentioning

confidence: 99%