SH2 domain-mediated activation of an SRC family kinase is not required to initiate Ca2+ release at fertilization in mouse eggs

Mehlmann, Lisa M.; Jaffe, Laurinda A.

doi:10.1530/rep.1.00638

Cited by 44 publications

(40 citation statements)

References 53 publications

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“…This difference was not significant as determined by Mann-Whitney rank sum test (PZ0.561). These results are consistent with the recently published experiments testing these fusion proteins in eggs activated by normal fertilization (Kurokawa et al 2004a, Mehlmann & Jaffe 2005 (Sakakibara et al 2005), the sperm-induced calcium transient (Abassi et al 2000), pronuclear migration and fusion (Moore & Kinsey 1995, Wright & Schatten 1995, as well as developmental competence (Livingston et al 1998, Sharma et al 2005. The extent to which these pathways are active in mammalian fertilization is now the subject of much investigation.…”

Section: Role Of Fyn In Mouse Egg Activationsupporting

confidence: 90%

“…For example, while mammalian eggs express Fyn, Yes, and in some cases, Src (Talmor et al 1998, Talmor-Cohen et al 2004a), the results of two different studies indicate that these kinases are not required for the unique sperm-induced calcium oscillations (Kurokawa et al 2004a, Mehlmann & Jaffe 2005, which trigger egg activation in mammals (Carroll 2001). Instead, these calcium oscillations are triggered directly by a sperm-borne phospholipase that does not require PTK regulation (Cox et al 2002).…”

Section: Introductionmentioning

confidence: 99%

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Role of Src homology 2 domain-mediated PTK signaling in mouse zygotic development

Meng

Luo²,

Li³

et al. 2006

Reproduction

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Fyn and other Src-family kinases play an essential role at several steps during egg activation following fertilization of externally fertilizing species, such as marine invertebrates, fish, and frogs. Recent studies demonstrate that the requirement for Src-family kinases in activation of the mammalian egg is different from lower species, and the objective of this study was to test the role of the Fyn kinase in the mouse egg activated by intracytoplasmic sperm injection (ICSI). An Src homology 2 (SH2) domain containing fusion protein was used to suppress Fyn function in the mouse zygote following ICSI. Eggs injected with the Fyn SH2 domain at an intracellular concentration of 4-8 mM exhibited reduced developmental potential with 100% of the zygotes being arrested following the first or the second cleavage. At higher concentrations, the protein blocked pronuclear congression and the zygotes remained at the pronuclear stage. The SH2 domain had no effect on sperm-induced calcium oscillations in distinct contrast to its effect on the eggs of lower species. The results indicate that the SH2 domain of Fyn kinase plays an important role in pronuclear congression as well as early cleavage events and that this effect appears not to involve disruption of calcium oscillations.

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Section: Role Of Fyn In Mouse Egg Activationsupporting

confidence: 90%

Section: Introductionmentioning

confidence: 99%

Role of Src homology 2 domain-mediated PTK signaling in mouse zygotic development

Meng

Luo²,

Li³

et al. 2006

Reproduction

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“…1A). In mouse, however, inhibition of SFKs in oocytes does not prevent Ca 2ϩ release upon fertilization, indicating that egg SFKs are not essential in the Ca 2ϩ release pathway (Mehlmann and Jaffe, 2005). Instead, current evidence supports the hypothesis that sperm fusion introduces an already-active PLC zeta (PLC ) from the sperm into the egg ( Fig.…”

Section: Triggers Of Egg Activation Spermmentioning

confidence: 52%

Transitioning from egg to embryo: Triggers and mechanisms of egg activation

Horner

Wolfner

2008

Developmental Dynamics

183

177

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The transition from mature oocyte to developing embryo requires a coordinated series of events, collectively known as egg activation. Egg activation includes changes to egg coverings to prevent polyspermy, release of oocyte meiotic arrest, generation of haploid female and male pronuclei, changes in maternal mRNAs and protein populations, and cytoskeletal rearrangements. In many animals, egg activation is triggered by fertilization, which increases intracellular calcium within the oocyte and thereby regulates molecular events of egg activation. In other animals, fertilization-independent external signals, including mechanical stimulation of eggs and/or changes in ionic milieu, trigger activation. Recent studies have clarified the upstream portion of pathways leading to eggshell changes and cell cycle resumption and have identified activation-induced changes in maternal mRNA and protein profiles that can identify molecular players in the downstream events of egg activation. We review signals that trigger activation and how they link to subsequent molecular events of egg activation. Developmental Dynamics 237:527-544, 2008.

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“…Src, Yes, and Fyn are found in a broad range of mammalian cells, including oocytes (Sato et al 1996, Talmor-Cohen et al 2004, Mehlmann & Jaffe 2005, Meng et al 2006, McGinnis et al 2007, Zheng et al 2007). …”

Section: Introductionmentioning

confidence: 99%

Fyn kinase is involved in cleavage furrow ingression during meiosis and mitosis

Levi¹,

Maro²,

Shalgi³

2010

Reproduction

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Fertilization of mammalian oocytes triggers their exit from the second meiotic division metaphase arrest. The extrusion of the second polar body (PBII) that marks the completion of meiosis is followed by the first mitotic cleavage of the zygote. Several lines of evidence in somatic cells imply the involvement of Fyn, an Src family kinase (SFK), in cell cycle control and actin functions. In this study, we demonstrate, using live cell confocal imaging and microinjection of Fyn cRNAs, the recruitment of Fyn to the oocyte's cortical area overlying the chromosomes and its colocalization with filamentous actin (F-actin) during exit from the meiotic metaphase. Fyn concentrated asymmetrically at the cortical site designated for ingression of the PBII cleavage furrow, where F-actin had already been accumulated, and then redispersed throughout the entire cortex only to be recruited again to the cleavage furrow during the first mitotic division. Although microinjection of dominant negative Fyn did not affect initiation of the cleavage furrow, it prolonged the average duration of ingression, decreased the rates of PB extrusion and of the first cleavage, and led to the formation of bigger PBs and longer spindles. Extrusion of the PBII was blocked in oocytes exposed to SU6656, an SFK inhibitor. Our results demonstrate, for the first time, a continuous colocalization of Fyn and F-actin during meiosis and imply a role for the SFKs, in general, and for Fyn, in particular, in regulating pathways that involve actin cytoskeleton, during ingression of the meiotic and mitotic cleavage furrows.

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SH2 domain-mediated activation of an SRC family kinase is not required to initiate Ca2+ release at fertilization in mouse eggs

Cited by 44 publications

References 53 publications

Role of Src homology 2 domain-mediated PTK signaling in mouse zygotic development

Role of Src homology 2 domain-mediated PTK signaling in mouse zygotic development

Transitioning from egg to embryo: Triggers and mechanisms of egg activation

Fyn kinase is involved in cleavage furrow ingression during meiosis and mitosis

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