SH2B1 and IRSp53 Proteins Promote the Formation of Dendrites and Dendritic Branches

Chen, Chien-Jen; Shih, Chien-Hung; Chang, Yu‐Jung; Hong, Shao-Jing; Li, Tian-Neng; Wang, Lily; Chen, Linyi

doi:10.1074/jbc.m114.603795

Cited by 25 publications

(23 citation statements)

References 36 publications

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“…It has been reported that CDC42 and IRSp53 are both potential candidate substrates of PTP1B, and PTP1B was identified two times in our intracellular data. Functionally, both PTP1B and IRSp53 have been linked to dendritic filopodia‐like formation in neurons, and a recent proteomics study identified that the enrichment of IRSp53 in dendritic filopodia . There have been reports showing that HSV‐1 dramatically increases the formation of dendritic filopodia to facilitate viral spread in primary neurons .…”

Section: Discussionmentioning

confidence: 99%

Proteomics Analysis Identifies IRSp53 and Fascin as Critical for PRV Egress and Direct Cell–Cell Transmission

Miao

Xia

et al. 2019

Proteomics

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Pseudorabies virus (PRV) has been widely used as a live trans‐synaptic tracer for mapping neuronal circuits. Systematically identifying mature PRV virion proteomes and defining co‐purified host proteins are necessary to fully understand the detailed mechanism underlying PRV transmission processes. Here, a PRV virion purification strategy based on sorting with flow cytometry is developed and the mature extracellular and intracellular PRV virion proteomes using LC coupled with MS/MS are characterized. In addition to viral proteins, a large number of host proteins are also identified, including proteins related to actin cytoskeletal dynamics and membrane protrusion. How many of these host proteins are true virion components are unknown and the majority of these may not be. Through functional analysis, it is found that IRSp53 and fascin are critical for the egress process and play a role in direct cell–cell transmission. Moreover, it is shown that CDC42 and Rac1 are also involved in the production of mature extracellular virions. The results suggest that the formation of the filopodia‐like cytoskeleton and the rearrangement of the membrane, which are both associated with IRSp53 and fascin, may be important for the transmission of viruses used in neuronal tracing.

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Section: Discussionmentioning

confidence: 99%

Proteomics Analysis Identifies IRSp53 and Fascin as Critical for PRV Egress and Direct Cell–Cell Transmission

Miao

Xia

et al. 2019

Proteomics

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“…Additionally, JAK2 has been reported to be regulated by SH2B1 adapter protein 1 (Maures, Kurzer, & Carter‐Su, ), which is able to increase tyrosine kinase activity that is associated with cytokine receptors, such as JAK2. Interestingly, Chen et al () suggested that SH2B1 protein orchestrates signaling for dendrite formation and its branching, both in hippocampal neurons and in the PC12 neuronal cell line.…”

Section: Discussionmentioning

confidence: 99%

Intracerebroventricular administration of growth hormone induces morphological changes in pyramidal neurons of the hippocampus and prefrontal cortex in adult rats

Olivares-Hernández

García-García

Camacho-Ábrego

et al. 2018

Synapse

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A growing body of evidence suggests that growth hormone (GH) affects synaptic plasticity at both the molecular and electrophysiological levels. However, unclear is whether plasticity that is stimulated by GH is associated with changes in neuron structure. This study investigated the effect of intracerebroventricular (ICV) administration of GH on the morphology of pyramidal neurons of the CA1 region of the dorsal hippocampus and layer III of the prefrontal cortex. Male Wistar rats received daily ICV injections of GH (120 ng) for 7 days, and they were euthanized 21 days later. Changes in neuronal morphology were evaluated using Golgi-Cox staining and subsequent Sholl analysis. GH administration increased total dendritic length in the CA1 region of the dorsal hippocampus and prefrontal cortex. The Sholl analysis revealed an increase in dendritic length of the third to eighth branch orders in the hippocampus and from the third to sixth branch orders in the prefrontal cortex. Interestingly, GH treatment increased the density of dendritic spines in both brain regions, favoring the presence of mushroom-like spines only in the CA1 hippocampal region. Our results indicated that GH induces changes in the length of dendritic trees and the density of dendritic spines in two high-plasticity brain regions, suggesting that GH-induced synaptic plasticity at the molecular and electrophysiological levels may be associated with these structural changes in neurons.

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“…Sprague Dawley (SD) rats were purchased from BioLASCO Taiwan Co., Ltd. Primary cortical neurons were dissociated from dissected cortices of rat embryos (E18), seeded onto PLL-coated dishes and culture in vitro as previously described [48,49]. For the in vitro TBI model, cortical neurons were injured by scraping with a p20 tip to generate gaps over the culture on DIV8 ( Figure 1A).…”

Section: In Vitro Culture Of Primary Neurons and The Tbi Modelmentioning

confidence: 99%

Epigenetic Regulation of WNT3A Enhancer during Regeneration of Injured Cortical Neurons

Chang

Hung

Huang

et al. 2020

IJMS

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Traumatic brain injury is known to reprogram the epigenome. Chromatin immunoprecipitation-sequencing of histone H3 lysine 27 acetylation (H3K27ac) and tri-methylation of histone H3 at lysine 4 (H3K4me3) marks was performed to address the transcriptional regulation of candidate regeneration-associated genes. In this study, we identify a novel enhancer region for induced WNT3A transcription during regeneration of injured cortical neurons. We further demonstrated an increased mono-methylation of histone H3 at lysine 4 (H3K4me1) modification at this enhancer concomitant with a topological interaction between sub-regions of this enhancer and with promoter of WNT3A gene. Together, this study reports a novel mechanism for WNT3A gene transcription and reveals a potential therapeutic intervention for neuronal regeneration.

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SH2B1 and IRSp53 Proteins Promote the Formation of Dendrites and Dendritic Branches

Cited by 25 publications

References 36 publications

Proteomics Analysis Identifies IRSp53 and Fascin as Critical for PRV Egress and Direct Cell–Cell Transmission

Proteomics Analysis Identifies IRSp53 and Fascin as Critical for PRV Egress and Direct Cell–Cell Transmission

Intracerebroventricular administration of growth hormone induces morphological changes in pyramidal neurons of the hippocampus and prefrontal cortex in adult rats

Epigenetic Regulation of WNT3A Enhancer during Regeneration of Injured Cortical Neurons

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