2002
DOI: 10.1038/nsb782
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SH3-dependent stimulation of Src-family kinase autophosphorylation without tail release from the SH2 domain in vivo

Abstract: Src family protein-tyrosine kinase activity is suppressed by two intramolecular interactions. These involve binding of the SH2 domain to the phosphorylated C-terminal tail and association of the SH3 domain with a polyproline type II helix formed by the SH2-kinase linker. Here we show that SH3-dependent activation of the Src family member Hck by HIV-1 Nef binding or by SH2-kinase linker mutation does not affect tail tyrosine phosphorylation in fibroblasts. Surprisingly, replacement of the wild type Hck tail wit… Show more

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Cited by 84 publications
(175 citation statements)
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“…4b). Similarly to our finding, Lerner and Smithgall also showed that human immunodeficiency virus-1 Nef proteininduced Hck (another Src family member) activation is not associated with a decrease in the phosphorylation of the conserved C-terminal tyrosine (35). It is possible that SH3 and/or SH2 domain ligand-induced activation of Src family protein tyrosine kinases is a direct effect and does not require the activation of protein tyrosine phosphatases.…”
Section: Mechanical Stretch-induced C-src Activation and C-srcsupporting
confidence: 61%
“…4b). Similarly to our finding, Lerner and Smithgall also showed that human immunodeficiency virus-1 Nef proteininduced Hck (another Src family member) activation is not associated with a decrease in the phosphorylation of the conserved C-terminal tyrosine (35). It is possible that SH3 and/or SH2 domain ligand-induced activation of Src family protein tyrosine kinases is a direct effect and does not require the activation of protein tyrosine phosphatases.…”
Section: Mechanical Stretch-induced C-src Activation and C-srcsupporting
confidence: 61%
“…S6). A number of in vitro and in vivo data including cell based assays and mutagenesis have also supported the concept of such an SH3-displaced model in kinase activation (5,27). While the present results are clearly at the limit of resolution available from the analysis of SAXS data, they support the concept of SH3-displaced states.…”
Section: Discussioncontrasting
confidence: 46%
“…Then what is the role of C3G in the induction of apoptosis? Activation of Hck by mutation of negative regulatory tyrosine phosphorylation site or by the SH3 ligand Nef results in fibroblast cell transformation (45)(46)(47). This is due to large increases in kinase activity of Hck.…”
Section: Discussionmentioning
confidence: 99%