ShaoYao decoction ameliorates colitis-associated colorectal cancer by downregulating proinflammatory cytokines and promoting epithelial-mesenchymal transition

Lin, Xiaochang; Yi, Zhiyong; Diao, Jiang; Shao, Meng; Zhao, Liang; Cai, Hongwei; Fan, Qian; Yao, Xueqing; Sun, Xuegang

doi:10.1186/1479-5876-12-105

Cited by 64 publications

(55 citation statements)

References 35 publications

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“…Thereby, tumor cells can penetrate the microvascular basic membrane more easily and be transported through the circulation to distant sites (6,30,31). Previous investigations have proven that some constituents of Chinese medicine function as suppressors of EMT (32)(33)(34). Our research team also confirmed that SYY inhibited the occurrence of EMT in HCC (20).…”

Section: Discussionsupporting

confidence: 71%

Astragaloside IV inhibits metastasis in hepatoma cells through the suppression of epithelial-mesenchymal transition via the Akt/GSK-3β/β-catenin pathway

Qin

Ren

et al. 2017

Oncology Reports

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Our previous studies demonstrated that traditional Chinese herbal medicine 'Songyou Yin' inhibited the growth and invasion of hepatocellular carcinoma (HCC) cells, and altered epithelial‑mesenchymal transition (EMT) markers in oxaliplatin‑treated HCC tissues and cell lines. In the present study, we aimed to explore whether astragaloside IV (AS-IV), a component of 'Songyou Yin', can affect the growth and invasion of HCC cells and the underlying mechanism involved. Human HCC cell lines Huh7 and MHCC97-H, with low and high metastatic potential, respectively, were treated with increasing doses of AS-IV. The Cell Counting Kit-8 (CCK-8), plate clone formation, Transwell, wound healing and immunofluorescence assays were used to investigate the effects of AS-IV on HCC cell proliferation, migration and invasion. The protein expression levels were analyzed by western blotting and immunofluorescence assay. The CCK-8 and plate clone formation assays showed that AS-IV had little effect on the proliferation of HCC cells in vitro. However, the Transwell and wound healing assays demonstrated that AS-IV inhibited the migration and invasion of HCC cells in a dose-dependent manner and the morphology of HCC cells was altered from spindle into oval shaped in the AS-IV pretreated groups. The upregulation of E-cadherin and downregulation of N-cadherin, vimentin, α-SMA and Slug were also observed in the AS-IV pretreated groups. Additionally, AS-IV treatment resulted in a profound decrease in the phosphorylated forms of Akt and GSK-3β, which in turn inhibited the expression of β-catenin. Thus, we conclude that AS-IV attenuates the invasive and migratory abilities of HCC cells through the inhibition of EMT by targeting the Akt/GSK-3β/β-catenin pathway.

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Section: Discussionsupporting

confidence: 71%

Astragaloside IV inhibits metastasis in hepatoma cells through the suppression of epithelial-mesenchymal transition via the Akt/GSK-3β/β-catenin pathway

Qin

Ren

et al. 2017

Oncology Reports

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“…Shaoyao decoction is a Chinese medicinal formula consisted of 9 herbs. Study shows that the dose of 7.12 g/ kg increases the survival rate of DSS-induced UC mice and attenuates the expression levels of serum IL-1β, IL-6, TNF-α and NF-κB activation [135].…”

Section: Heat-clearing and Drying Dampness Categorymentioning

confidence: 94%

“…Suppress NF-κB activation [135] Qingchang Huashi recipe HT-29 cells Reduce the activation of NF-κB [137] Gegenqinlian Decoction TNBS-induced colon tissue Inhibit the activation and translocation of NF-κBp65 [138] DSS-induced colonic tissues Decrease the P-NF-κBp65 [139] RAW 264.7 cells Down-regulate the expression of P-NF-κBp65 [139] Jianpi Qingchang decoction DSS-induced colon tissue Inhibit the activation of the NF-κB [140] Modified pulsatilla decoction OXZ-induced colon tissues Suppress the activation of the NF-κB [142] Qing Hua Chang Yin DSS-induced colon tissue Inhibit the expression of the nuclear translocation of NF-κB [143] QingBai decoction DSS-induced colon tissue Decrease the protein level of P-NF-κBp65 [145] Chang-An-Shuan TNBS-induced colonic tissues Down-regulate the expression levels of NF-κBp65 [146] Baishaoqiwu TNBS-induced colon tissues Inhibit expression of the NF-ĸBp65 genes [147] Xie-xin decoction TNBS-induced colon tissues Decrease expression of NF-ĸBp65 [148] Compound sophorae decoction…”

Section: Shaoyao Decoction Aom/dss-induced Colonic Tissuesmentioning

confidence: 99%

Targeting NF-κB pathway for treating ulcerative colitis: comprehensive regulatory characteristics of Chinese medicines

Zhao

2020

Chin Med

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Nuclear factor-kappa B (NF-κB) is a kind of multi-functional nuclear transcription factor involved in regulating gene transcription to influence pathological evolution of inflammatory and immune diseases. Numerous literature evidence that NF-κB pathway plays an essential role in pathogenic development of ulcerative colitis (UC). UC is a chronic non-specific inflammatory bowel disease, and until now, therapeutic agents for UC including aminosalicylates, corticosteroids and immune inhibitors still cannot exert satisfied effects on patients. In recent years, Chinese medicines suggest the advantages of alleviating symptoms and signs, decreasing side-effects and recurrence, whose one of mechanisms is related to regulation of NF-κB pathway. In this review, we categorize Chinese medicines according to their traditional therapeutic functions, and summarize the characteristics of Chinese medicines targeting NF-κB pathway in UC treatment. It indicates that 85 kinds of Chinese medicines' compounds and formulae can directly act on NF-κBp65; while 58 Chinese medicines' ingredients and formulae indirectly suppress NF-κBp65 by regulation of its upstream or other related pathways. Moreover, by the analysis of Chinese medicines' category based on their traditional functions, we conclude the category of dampness-drying and detoxificating medicine targeting NF-κB pathway accounts for primary status for amelioration of UC. Simultaneously, this review also contributes to the choices of Chinese medicine category and provides curative potential of Chinese medicines for clinical UC treatment.

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“…This cycle was repeated twice. YTE‐17 (25 mg/kg/day) and Aspirin (30 mg/kg) were given orally for 12 weeks during the DSS treatment separately as previously described 1 . Aspirin is taken as a positive control, because it showed effectively inhibiting effect on intestinal adenomatous polyposis and colon carcinogenesis 22,23 .…”

Section: Methodsmentioning

confidence: 99%

The active fraction of Garcinia yunnanensis suppresses the progression of colorectal carcinoma by interfering with tumorassociated macrophage‐associated M2 macrophage polarization in vivo and in vitro

Sui

Tan

et al. 2020

FASEB j.

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Colorectal cancer (CRC) is the third most common solid tumor worldwide and has shown resistance to several immunotherapies, particularly immune checkpoint blockade therapy, which is effective in many other types of cancer. Our previous studies indicated that the active fraction of Garcinia yunnanensis (YTE‐17), had potent anticancer activities by regulating multiple signaling pathways. However, knowledge regarding the mechanism and effect of YTE‐17 in the prevention of CRC is limited. This study tested the effects of YTE‐17 on colon cancer development in vivo by using two murine models: the carcigenic azoxymethane/dextran sulfate sodium (AOM/DSS)‐induced CRC model and a genetically induced model using ApcMin/+ mice. Here, the tumor load, tumor number, histology, and even some oncogenes were used to evaluate the effect of YTE‐17. The intragastric administration of YTE‐17 for 12 weeks significantly decreased CRC incidence, tumor number and size, immunity, and some tumor‐associated macrophage (TAM) markers, including CD206, Arg‐1, IL‐10, and TGF‐β. Importantly, the macrophages depletion by clodronate (CEL) also played a role in reducing the tumor burden and inhibiting tumor development, which were not affected by YTE‐17 in the ApcMin/+ mice. Moreover, the YTE‐17 treatment attenuated CRC cell growth in a co‐culture system in the presence of macrophages. Consistently, YTE‐17 effectively reduced the tumor burden and macrophage infiltration and enhanced immunity in the AOM/DSS and ApcMin/+ colon tumor models. Altogether, we demonstrate that macrophages in the microenvironment may contribute to the development and progression of CRC cells and propose YTE‐17 as a new potential drug option for the treatment of CRC.

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ShaoYao decoction ameliorates colitis-associated colorectal cancer by downregulating proinflammatory cytokines and promoting epithelial-mesenchymal transition

Cited by 64 publications

References 35 publications

Astragaloside IV inhibits metastasis in hepatoma cells through the suppression of epithelial-mesenchymal transition via the Akt/GSK-3β/β-catenin pathway

Astragaloside IV inhibits metastasis in hepatoma cells through the suppression of epithelial-mesenchymal transition via the Akt/GSK-3β/β-catenin pathway

Targeting NF-κB pathway for treating ulcerative colitis: comprehensive regulatory characteristics of Chinese medicines

The active fraction of Garcinia yunnanensis suppresses the progression of colorectal carcinoma by interfering with tumorassociated macrophage‐associated M2 macrophage polarization in vivo and in vitro

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