Shape deformation of giant vesicles encapsulating charged colloidal particles

Natsume, Yuno; Pravaz, Olivier; Yoshida, Hiroyuki; Imai, Masayuki

doi:10.1039/c0sm00396d

Cited by 32 publications

(46 citation statements)

References 36 publications

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“…1F). We occasionally observed a transformation similar to the pearling instability reported previously (37,49). This observation is in agreement with the model that budding should continue until v red of each vesicle approaches one, where no excess membrane for deformation remains.…”

Section: Resultssupporting

confidence: 92%

“…Because the change in depletion volume occurring with the shape transformation is extremely small (the thickness equals the gyration radius of polymers, 1-10 nm), it is difficult to evaluate from the experimentally observed shapes. Thus, we estimated it by approximating the vesicle shapes to mathematically definable geometries (37,50). In our experiment, we often observed a transformation from the elongated tube to the doublet (budded) shape at the last stage of transformation (Fig.…”

Section: Resultsmentioning

confidence: 96%

“…Thus, the shapes with positive curvatures are thermodynamically favored if the gain of the entropy (reduction of the free energy) due to the increase of V free overcomes the energy necessary to bend the membrane. Recently, this hypothesis was tested using GUVs containing microbeads (1-μm diameter) at a volume fraction of approximately 50% (37). However, this effect has not been studied with nanometric macromolecules, which are more biolo- gically relevant.…”

Section: Resultsmentioning

confidence: 99%

“…We show that budding can be induced by encapsulating inert polymer molecules, which mimic cytosolic macromolecules. This membrane deformation is driven by maximization of the translational entropy of the polymers in the vesicle (37). The combination of these entirely physical and nonspecific effects enables the recursive cycles of fusion and budding of giant lipid vesicles.…”

mentioning

confidence: 99%

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Coupling of the fusion and budding of giant phospholipid vesicles containing macromolecules

Terasawa

Nishimura

Suzuki

et al. 2012

Proc. Natl. Acad. Sci. U.S.A.

160

166

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Mechanisms that enabled primitive cell membranes to self-reproduce have been discussed based on the physicochemical properties of fatty acids; however, there must be a transition to modern cell membranes composed of phospholipids [Budin I, Szostak JW (2011) Proc Natl Acad Sci USA 108:5249–5254]. Thus, a growth-division mechanism of membranes that does not depend on the chemical nature of amphiphilic molecules must have existed. Here, we show that giant unilamellar vesicles composed of phospholipids can undergo the coupled process of fusion and budding transformation, which mimics cell growth and division. After gaining excess membrane by electrofusion, giant vesicles spontaneously transform into the budded shape only when they contain macromolecules (polymers) inside their aqueous core. This process is a result of the vesicle maximizing the translational entropy of the encapsulated polymers (depletion volume effect). Because the cell is a lipid membrane bag containing highly concentrated biopolymers, this coupling process that is induced by physical and nonspecific interactions may have a general importance in the self-reproduction of the early cellular compartments.

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Section: Resultssupporting

confidence: 92%

Section: Resultsmentioning

confidence: 96%

Section: Resultsmentioning

confidence: 99%

mentioning

confidence: 99%

See 2 more Smart Citations

Coupling of the fusion and budding of giant phospholipid vesicles containing macromolecules

Terasawa

Nishimura

Suzuki

et al. 2012

Proc. Natl. Acad. Sci. U.S.A.

160

166

View full text Add to dashboard Cite

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“…Many studies on the shape changes of these capsules by the confinement effect have been reported for vesicles [5 -9] and microemulsion droplets. [10 -16] Natsume et al [5] reported that the shapes of giant vesicles were transformed to multi-bead shapes upon the encapsulation of negatively charged colloidal particles, and they clarified the mechanism of transformation to the multi-bead shapes by considering the free volumes for the particles inside the vesicles. In the case of a microemulsion droplet system, Nakaya et al [10,11] encapsulated watersoluble polymers (gelatine) as guest molecules in the water-in-oil droplets by using solvent selectivity, and observed the transformations of the spherical droplet shapes to rod-like, worm-like and, finally, network-like shapes.…”

Section: Introductionmentioning

confidence: 99%

Molecular dynamics simulation for shape change of water-in-oil droplets

Urakami

Takaki

Imai

et al. 2014

Molecular Simulation

Self Cite

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We performed molecular dynamics (MD) simulations of water-in-oil droplet shape transformations induced by the addition of polymer chains. In a prior experiment, transformations of spherical droplets to rod-like, worm-like and network-like droplets were observed. In our previous study, we reproduced rod-like droplets via coarse-grained MD simulations, and the mechanism for the droplet shape change was elucidated by considering the contact area between the chains and the surfactant head groups. However, in that simulation model, we could not reproduce the worm-like and network-like droplets. In this study, we improved the simulation model. For a small number of chains, several spherical droplets were obtained. As the number of chains increased, the spherical droplets were transformed to rod-like, worm-like and networklike shapes by coalescence of the droplets. The calculated and experimental results agreed well, and we verified that the mechanism for the droplet shape transformations observed in the present simulations could be explained by the mechanism suggested in the previous study.

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Poly(Ionic Liquid) Nanoparticles Selectively Disrupt Biomembranes

et al. 2018

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Polymer‐based nanoparticles have an increasing presence in research due to their attractive properties, such as flexible surface functionality design and the ability to scale up production. Poly(ionic liquid) (PIL) nanoparticles of size below 50 nm are very unique in terms of their high charge density and internal onion‐like morphology. The interaction between PIL nanoparticles and giant unilamellar vesicles (GUVs) of various surface charge densities is investigated. GUVs represent a convenient model system as they mimic the size and curvature of plasma membranes, while simultaneously offering direct visualization of the membrane response under the microscope. Incubating PIL nanoparticles with GUVs results in poration of the lipid membrane in a concentration‐ and charge‐dependent manner. A critical poration concentration of PILs is located and the interactions are found to be analogous to those of antimicrobial peptides. Microbial mimetic membranes are already affected at submicromolar PIL concentrations where contrast loss is observed due to sugar exchange across the membrane, while at high concentrations the collapse of vesicles is observed. Finally, a confocal microscopy–based approach assessing the particle permeation through the membrane is reported and a mechanism based on bilayer frustration and pore stabilization via particle integration in the membrane is proposed.

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Shape deformation of giant vesicles encapsulating charged colloidal particles

Cited by 32 publications

References 36 publications

Coupling of the fusion and budding of giant phospholipid vesicles containing macromolecules

Coupling of the fusion and budding of giant phospholipid vesicles containing macromolecules

Molecular dynamics simulation for shape change of water-in-oil droplets

Poly(Ionic Liquid) Nanoparticles Selectively Disrupt Biomembranes

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