Shape of gastrointestinal immunity with non-genetically modified Lactococcus lactis particles requires commensal bacteria and myeloid cells-derived TGF-β1

Zeng, Zhiqing; Liu, Wei; Luo, Shuanghui; Hu, Chupeng; Xu, Ningyin; Huang, An; Xi, Tao; Xing, Yingying

doi:10.1007/s00253-019-09716-z

Cited by 9 publications

(7 citation statements)

References 35 publications

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“…Purified CTB-UE-CF (CCF) protein was constructed by cholera toxin B, multi-epitopes from H. pylori urease, and self-adjuvant regions from S typhimurium phase I flagellin FliC, and the preparation of CCF was undertaken as described previously [ 19 ]. Briefly, CCF protein was expressed by Escherichia coli Rosetta (DE3) cells with pET-28a-CCF.…”

Section: Methodsmentioning

confidence: 99%

Silk fibroin hydrogel as mucosal vaccine carrier: induction of gastric CD4+TRM cells mediated by inflammatory response induces optimal immune protection against Helicobacter felis

Liu

et al. 2020

Emerging Microbes & Infections

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Tissue-resident memory T (T RM ) cells, located in the epithelium of most peripheral tissues, constitute the first-line defense against pathogen infections. Our previous study reported that gastric subserous layer (GSL) vaccination induced a “pool” of protective tissue-resident memory CD4+T (CD4+T RM ) cells in the gastric epithelium. However, the mechanistic details how CD4+T RM cells form in the gastric epithelium are unknown. Here, our results suggested that the vaccine containing CCF in combination with Silk fibroin hydrogel (SF) broadened the distribution of gastric intraepithelial CD4+T RM cells. It was revealed that the gastric intraepithelial T RM cells were even more important than circulating memory T cells against infection by Helicobacter felis . It was also shown that gastric-infiltrating neutrophils were involved as indispensable mediators which secreted CXCL10 to chemoattract CXCR3+CD4+T cells into the gastric epithelium. Blocking of CXCR3 or neutrophils significantly decreased the number of gastric intraepithelial CD4+T RM cells due to reduced recruitment of CD4+T cells. This study demonstrated the protective efficacy of gastric CD4+T RM cells against H. felis infection, and highlighted the influence of neutrophils on gastric intraepithelial CD4+T RM cells formation.

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Section: Methodsmentioning

confidence: 99%

Silk fibroin hydrogel as mucosal vaccine carrier: induction of gastric CD4+TRM cells mediated by inflammatory response induces optimal immune protection against Helicobacter felis

Liu

et al. 2020

Emerging Microbes & Infections

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“…After centrifugation, the cells were washed with 5 mL PBS. The single cells from the lymph nodes were collected straightway after centrifugation and washing …”

Section: Methodsmentioning

confidence: 99%

Vaccine‐induced gastric CD4⁺ tissue‐resident memory T cells proliferate in situ to amplify immune response against Helicobacter pylori insult

Ruan

Liu

et al. 2019

Helicobacter

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Background: Tissue-resident memory T cells accelerate the clearance of pathogens during recall response. However, whether CD4 + TRM cells themselves can provide gastric immunity is unclear. Materials and methods:We established a parabiosis model between the enhanced green fluorescent protein and wild-type mice that the circulation system was shared, and the wild-type partner was vaccinated with H pylori vaccine composed of CCF and silk fibroin in gastric subserous layer to induce gastric EGFP + CD4 + TRM cells. Antigen-specific EGFP+ CD4 + T cells and proliferous TRM cells were analyzed by flow cytometry. The colonization of H pylori was detected by quantitative real-time PCR. EGFP + CD4 + TRM cells and the inflammation of the stomach were observed by histology.Results: A parabiosis animal model was employed to identify the cells that introduced by vaccination in GSL. Antigen-specific EGFP + CD4 + T cells could be detected at day 7 post-vaccination. Thirty days later, EGFP + CD4 + TRM cells were established with a phenotype of CD69 + CD103 -. Of note, we found that when circulating lymphocytes were depleted by FTY720 administration, these TRM cells could proliferate in situ and differentiate into effector Th1 cells after H pylori challenge. A decrease in H pylori colonization was observed in the vaccinated mice but not unvaccinated mice.Further, we found that although FTY720 was administrated, mounted pro-inflammatory myeloid cells still emerged in the stomach of the vaccinated mice, which might contribute to the reduction of H pylori colonization. Conclusions:Our study reveals that H pylori vaccine-induced CD4 + TRM cells can proliferate and differentiate in situ to enhance gastric local immunity during recall response.

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“…H. felis (ATCC 49179) was cultured as previously described [7,28]. H. felis was routinely cultured at 37 °C with 120 rpm shaking in trypticase soy liquid medium supplemented with 10% heat-inactivated fetal bovine serum (FBS) under microaerophilic conditions.…”

Section: H Felis Challengementioning

confidence: 99%

CD4+ CD8αα+ T Cells in the Gastric epithelium Mediate Chronic Inflammation Induced by Helicobacter Felis

Ruan

Huang

et al. 2021

Preprint

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CD4+ CD8αα+ double-positive intraepithelial T lymphocytes (DP T cells), a newly characterized subset of intraepithelial T cell, has been reported to contribute to local immunosuppression. However, whether DP T cells are present in Helicobacter. pylori-induced gastritis, and their relationship with disease prognosis remain to be elucidated. In this study, We established chronic gastritis models through Helicobacter felis (H. felis) infection. Gastric infiltrating lymphocytes were isolated from H. felis-induced gastritis mice and analyzed by flow cytometry. Our results suggest that DP T cells frequency in H. felis-induced gastritis mice was higher than the uninfected mice. Gastric DP T cells derived from lamina propria cells, which distributed in the gastric epithelial layer. We found that DP T cells exhibited anti-inflammatory function. In vitro, DP T cells inhibited the maturation of dendritic cells and the proliferation of CD4+ T cell. The elimination of CD4+CD8αα+ T cells in vivo resulted in severe gastritis and a reduction of H. felis load. Additionally, vaccine with silk fibroin as delivery systems enhanced vaccine efficacy by reducing DP T cells. We demonstrated that DP T cells performed an immunosuppressive role in Helicobacter felis-induced gastritis. These findings revealed that DP T cells may affect the prognosis of the disease and the vaccine efficacy.

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Shape of gastrointestinal immunity with non-genetically modified Lactococcus lactis particles requires commensal bacteria and myeloid cells-derived TGF-β1

Cited by 9 publications

References 35 publications

Silk fibroin hydrogel as mucosal vaccine carrier: induction of gastric CD4+TRM cells mediated by inflammatory response induces optimal immune protection against Helicobacter felis

Silk fibroin hydrogel as mucosal vaccine carrier: induction of gastric CD4+TRM cells mediated by inflammatory response induces optimal immune protection against Helicobacter felis

Vaccine‐induced gastric CD4⁺ tissue‐resident memory T cells proliferate in situ to amplify immune response against Helicobacter pylori insult

CD4+ CD8αα+ T Cells in the Gastric epithelium Mediate Chronic Inflammation Induced by Helicobacter Felis

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Shape of gastrointestinal immunity with non-genetically modified Lactococcus lactis particles requires commensal bacteria and myeloid cells-derived TGF-β1

Cited by 9 publications

References 35 publications

Silk fibroin hydrogel as mucosal vaccine carrier: induction of gastric CD4+TRM cells mediated by inflammatory response induces optimal immune protection against Helicobacter felis

Silk fibroin hydrogel as mucosal vaccine carrier: induction of gastric CD4+TRM cells mediated by inflammatory response induces optimal immune protection against Helicobacter felis

Vaccine‐induced gastric CD4+ tissue‐resident memory T cells proliferate in situ to amplify immune response against Helicobacter pylori insult

CD4+ CD8αα+ T Cells in the Gastric epithelium Mediate Chronic Inflammation Induced by Helicobacter Felis

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Vaccine‐induced gastric CD4⁺ tissue‐resident memory T cells proliferate in situ to amplify immune response against Helicobacter pylori insult