Shaping and properties of thermoplastic scaffolds in tissue regeneration: The effect of thermal history on polymer crystallization, surface characteristics and cell fate

Calore, Andrea Roberto; Srinivas, Varun; Anand, Shivesh; Albillos-Sanchez, Ane; Looijmans, Stan F.S.P.; Breemen, Lambèrt C.A. van; Mota, Carlos; Bernaerts, Katrien V.; Harings, Jules; Moroni, Lorenzo

doi:10.1557/s43578-021-00403-2

Cited by 29 publications

(10 citation statements)

References 77 publications

(104 reference statements)

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“…Gelatin's primary functional groups are composed of carboxylic, amide, ester, aromatic and alkane 35 . Data from previous study suggested that a high crystallinity biomaterial demonstrated a high degree of stiffness, which may in turn inhibit cell attachment 36 . Nonetheless, our results demonstrated a lower intensity of amide peak upon genipin induced gelatin crosslinking via the free amide group, but the amorphous structure remained, which may facilitate the growth of MSCs.…”

Section: Discussionmentioning

confidence: 97%

In vitro evaluation of genipin-crosslinked gelatin hydrogels for vocal fold injection

Lokanathan

Fauzi

et al. 2023

Sci Rep

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Glottic insufficiency is one of the voice disorders affecting all demographics. Due to the incomplete closure of the vocal fold, there is a risk of aspiration and ineffective phonation. Current treatments for glottic insufficiency include nerve repair, reinnervation, implantation and injection laryngoplasty. Injection laryngoplasty is favored among these techniques due to its cost-effectiveness and efficiency. However, research into developing an effective injectable for the treatment of glottic insufficiency is currently lacking. Therefore, this study aims to develop an injectable gelatin (G) hydrogel crosslinked with either 1-ethyl-3-(3-dimethylaminpropyl)carbodiimide hydrochloride) (EDC) or genipin (gn). The gelation time, biodegradability and swelling ratio of hydrogels with varying concentrations of gelatin (6–10% G) and genipin (0.1–0.5% gn) were investigated. Some selected formulations were proceeded with rheology, pore size, chemical analysis and in vitro cellular activity of Wharton's Jelly Mesenchymal Stem Cells (WJMSCs), to determine the safety application of the selected hydrogels, for future cell delivery prospect. 6G 0.4gn and 8G 0.4gn were the only hydrogel groups capable of achieving complete gelation within 20 min, exhibiting an elastic modulus between 2 and 10 kPa and a pore size between 100 and 400 μm. Moreover, these hydrogels were biodegradable and biocompatible with WJMSCs, as > 70% viability were observed after 7 days of in vitro culture. Our results suggested 6G 0.4gn and 8G 0.4gn hydrogels as potential cell encapsulation injectates. In light of these findings, future research should focus on characterizing their encapsulation efficiency and exploring the possibility of using these hydrogels as a drug delivery system for vocal fold treatment.

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Section: Discussionmentioning

confidence: 97%

In vitro evaluation of genipin-crosslinked gelatin hydrogels for vocal fold injection

Lokanathan

Fauzi

et al. 2023

Sci Rep

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“…Paralelamente, os polímeros sintéticos representam uma escolha de aplicabilidade de scaffolds nos tecidos duros possuindo características físicas como rigidez do material, rugosidade e topografia desejáveis para a neoformação celular (CALORE et al, 2021).…”

Section: Discussionunclassified

Cicatrização De Lesão Por Pressão Tratada Com Película De Biocelulose: Relato De Caso

Turetta¹,

Cardoso²,

Oliveira³

et al. 2023

Ciências Da Saúde Teorias E Práticas

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Incomensurável é a gratidão que sinto em ser convidado pela brilhante Editora Brazilian Journals que promove a edição de e-books organizados por capítulos, fazer a apresentação do e-book: ""Ciências da saúde: teorias e práticas vol. 02", onde tive a grata satisfação de poder contribuir com 2 capítulos: "LEUCEMIA LINFOCÍTICA CRÔNICA DE CÉLULAS PILOSAS OU TRICOLEUCEMIA" e " IMPACTO DA OBESIDADE, HIPERTENSÃO ARTERIAL E DIABETES MELLITUS NA RECUPERAÇÃO``. Após a leitura dos 22 capítulos da revista percebo que apesar das adversidades que nós produtores de conteúdo científicos enfrentamos, para buscar dados científicos com evidências confiáveis, análise dos dados e sintetização efetiva do artigo científico, me sinto honrado em ver uma obra que engloba as diversas áreas das ciências da saúde. Assim, demonstra que estamos trilhando o caminho correto, de divulgar e aplicar os conhecimentos de Saúde. Dessa forma, construindo um futuro mais próspero com base no ensino, pesquisa e colaboração entre pesquisadores e população. Que tenhamos mais capítulos e livros publicados por esta editora. Congratulações para a equipe editorial e para todos os autores e colaboradores deste grande projeto.Boa leitura,

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“…Thermoplastic semi-crystalline polymers are excellent candidates for developing tissue-engineered scaffolds as they are easy to process and present adjustable properties. In parallel, synthetic polymers can be an option for scaffolds applicability in hard tissues, presenting physical characteristics such as material stiffness, roughness, and topography that are desirable for cell neoformation (Calore et al, 2021). Calore et al (2021) indicated that in an osteogenic environment, human mesenchymal stromal cells responded more to surface roughness than to surface stiffness of different scaffolds.…”

Section: Discussionmentioning

confidence: 99%

Grafting in peri-implant bone defects by in-situ polymer deposition using a 3D pen – in vitro/ ex vivo study

Moraes

LOURENÇONE

Goulart

et al. 2022

RSD

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Guided Bone Regeneration (GBR) aims to gain or maintain bone volume due to the use of barrier membranes that act for this purpose. This research aims at grafting polymeric filaments into preformed peri-implant bone defects in porcine condyles in vitro/ex vivo, stabilized and grafted with poly(lactic acid) (PLA) and poly(vinyl alcohol) (PVA) polymeric filaments, printed in-situ with a 3D printing pen. Nine porcine condyles received bone defects of 8 mm diameter and 7 mm depth, where occurred the installation of conical implants of 3.5x10 mm. After forming the bone gap region, above the apical bone anchorage, we divided the Poof Bodies (PB) according to the polymeric fill used: G.Control – without filling in the bone gap; G.PLA – with PLA scaffolds and G.PVA – with PVA scaffolds. In another step, the PVA and PLA 3D membranes were compared with the dense polytetrafluoroethylene membrane (PTFE-d). Subsequently, the SkyScan 1172 microtomograph (Bruker-μCT, Kontich, Belgium) analyzed the PB. The analysis corresponding to the total porosity revealed no statistical difference between G.Control (70.44%), G.PLA (59.99%), and G.PVA (57.66%). The closed porosity showed a statistical difference between G.Control (75.509%) and G.PVA (189.19%) and between G.PVA and G.PLA (79,093%). This study demonstrated the possibility of the polymeric filaments of PVA and PLA to fill the bone defects created, revealing an intimate contact on the surface of the implants used. The data suggested a higher porosity of the PVA filament when applied to bone defects or membrane shape.

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Shaping and properties of thermoplastic scaffolds in tissue regeneration: The effect of thermal history on polymer crystallization, surface characteristics and cell fate

Cited by 29 publications

References 77 publications

In vitro evaluation of genipin-crosslinked gelatin hydrogels for vocal fold injection

In vitro evaluation of genipin-crosslinked gelatin hydrogels for vocal fold injection

Cicatrização De Lesão Por Pressão Tratada Com Película De Biocelulose: Relato De Caso

Grafting in peri-implant bone defects by in-situ polymer deposition using a 3D pen – in vitro/ ex vivo study

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