Shared Genetic and Epigenetic Mechanisms between the Osteogenic Differentiation of Dental Pulp Stem Cells and Bone Marrow Stem Cells

Gaus, Sebastian; Li, Hanluo; Li, Simin; Wang, Qian; Kottek, Tina; Hahnel, Sebastian; Liu, Xiangqiong; Deng, Yupei; Ziebolz, Dirk; Haak, Rainer; Schmalz, Gerhard; Liu, Lei; Savkovic, Vuk; Lethaus, Bernd

doi:10.1155/2021/6697810

Cited by 19 publications

(16 citation statements)

References 100 publications

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“…13 A previous study has demonstrated that naringin facilitates osteogenic differentiation of bone mesenchymal stem cells (BMSCs), thus facilitating bone formation and alleviating osteoporosis in ovariectomy (OVX)-induced osteoporotic mice. 14 Previously, naringin was reported to exert protective effects against ankylosing spondylitis in mice via the induction of ossification, suppression of oxidative stress and inflammation, which were mediated by the inhibition of the Janus activated kinase 2 (JAK2)/signal transducer and the activator of transcription 3 (STAT3) pathway. 15 The JAK2/STAT3 pathway was shown to play a pivotal role in the initiation and development of osteoporosis.…”

Section: Introductionmentioning

confidence: 99%

Naringin promotes osteogenesis and ameliorates osteoporosis development by targeting JAK2/STAT3 signalling

Wang

Mao

Chen

et al. 2021

Clin Exp Pharma Physio

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Osteoporosis is a systemic bone metabolism disorder, which increases the risk of fractures, and in severe cases it may cause disability or even death. An important factor contributing to osteoporosis is the imbalance between bone formation and resorption. Naringin was reported to promote osteoblast differentiation, thus enhancing bone formation and alleviating osteoporosis development. However, the signalling pathways related to the regulatory mechanism of naringin in osteoporosis development are not clear. Proliferation of bone mesenchymal stem cells (BMSCs) treated with naringin in vitro was detected by CCK-8. An osteogenesis differentiation medium supplemented with naringin was applied to explore the effects of naringin on BMSC osteogenic differentiation, as detected by Alizarin red staining. Ovariectomy (OVX)induced postmenopausal osteoporosis (PMOP) rats were orally administered with naringin. Dual-energy X-ray absorptiometry (DEXA) and micro-CT were applied to measure bone mineral density (BMD), bone volume/total volume (BV/TV), trabecula thickness (Tb.Th), trabecula number (Tb.N), trabecular separation (Tb.Sp) and bone surface/bone volume (BS/BV). H&E staining was performed to show pathological changes of the femur in PMOP rats after naringin treatment. Bone metabolism indicators were assessed by ELISA. We found that naringin suppressed the activation of the JAK2/STAT3 pathway. Naringin promoted BMSC proliferation and osteogenic differentiation. Furthermore, naringin alleviates bone loss and improves abnormal bone metabolism of PMOP rats. Collectively, naringin promotes BMSC osteogenic differentiation to ameliorate osteoporosis development by targeting JAK2/STAT3 signalling.

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Section: Introductionmentioning

confidence: 99%

Naringin promotes osteogenesis and ameliorates osteoporosis development by targeting JAK2/STAT3 signalling

Wang

Mao

Chen

et al. 2021

Clin Exp Pharma Physio

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“…The miR-146 family is highly conserved between fish and humans, and it shares the same seed sequence [52]. In humans, it has been demonstrated that miR146b is downregulated in both bone marrow stem cell (BMSC) chondrogenesis [53] and osteogenesis [54]. In addition, it has been demonstrated that miR146b promotes adipogenesis too [55].…”

Section: Discussionmentioning

confidence: 99%

Methylsulfonylmethane enhances MSC chondrogenic commitment and promotes pre-osteoblasts formation

et al. 2021

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Background Methylsulfonylmethane (MSM) is a nutraceutical compound which has been indicated to counteract osteoarthritis, a cartilage degenerative disorder. In addition, MSM has also been shown to increase osteoblast differentiation. So far, few studies have investigated MSM role in the differentiation of mesenchymal stem cells (MSCs), and no study has been performed to evaluate its overall effects on both osteogenic and chondrogenic differentiation. These two mutually regulated processes share the same progenitor cells. Methods Therefore, with the aim to evaluate the effects of MSM on chondrogenesis and osteogenesis, we analyzed the expression of SOX9, RUNX2, and SP7 transcription factors in vitro (mesenchymal stem cells and chondrocytes cell lines) and in vivo (zebrafish model). Real-time PCR as well Western blotting, immunofluorescence, and specific in vitro and in vivo staining have been performed. Student’s paired t test was used to compare the variation between the groups. Results Our data demonstrated that MSM modulates the expression of differentiation-related genes both in vitro and in vivo. The increased SOX9 expression suggests that MSM promotes chondrogenesis in treated samples. In addition, RUNX2 expression was not particularly affected by MSM while SP7 expression increased in all MSM samples/model analyzed. As SP7 is required for the final commitment of progenitors to preosteoblasts, our data suggest a role of MSM in promoting preosteoblast formation. In addition, we observed a reduced expression of the osteoclast-surface receptor RANK in larvae and in scales as well as a reduced pERK/ERK ratio in fin and scale of MSM treated zebrafish. Conclusions In conclusion, our study provides new insights into MSM mode of action and suggests that MSM is a useful tool to counteract skeletal degenerative diseases by targeting MSC commitment and differentiation.

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“…Similar effects, i.e., enhanced mineralization and ALP activity with minimal (<50%) or no significant changes in osteogenic gene expression, have been previously reported in MC3T3-E1 osteoblasts treated with zinc and genistein [ 60 ]. Furthermore, curcumin appears to play a role as an epigenetic modulator in general [ 43 ] and specifically as an epigenetic modulator of miRNAs involved in the osteogenic differentiation of dental pulp stem cells [ 43 , 61 ]. Going forward, we suggest focusing on the mechanisms by which the three nutraceuticals studied can improve bone health in microgravity, for example, by altering post-translational regulation of the transcriptional activity of osteogenic genes or serving as modulators of the epigenetics of osteogenic miRNAs.…”

Section: Discussionmentioning

confidence: 99%

Nutraceuticals Synergistically Promote Osteogenesis in Cultured 7F2 Osteoblasts and Mitigate Inhibition of Differentiation and Maturation in Simulated Microgravity

Braveboy-Wagner

Sharoni

Lelkes

2021

IJMS

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Microgravity is known to impact bone health, similar to mechanical unloading on Earth. In the absence of countermeasures, bone formation and mineral deposition are strongly inhibited in Space. There is an unmet need to identify nutritional countermeasures. Curcumin and carnosic acid are phytonutrients with anticancer, anti-inflammatory, and antioxidative effects and may exhibit osteogenic properties. Zinc is a trace element essential for bone formation. We hypothesized that these nutraceuticals could counteract the microgravity-induced inhibition of osteogenic differentiation and function. To test this hypothesis, we cultured 7F2 murine osteoblasts in simulated microgravity (SMG) in a Random Positioning Machine in the presence and absence of curcumin, carnosic acid, and zinc and evaluated cell proliferation, function, and differentiation. SMG enhanced cell proliferation in osteogenic medium. The nutraceuticals partially reversed the inhibitory effects of SMG on alkaline phosphatase (ALP) activity and did not alter the SMG-induced reduction in the expression of osteogenic marker genes in osteogenic medium, while they promoted osteoblast proliferation and ALP activity in the absence of traditional osteogenic media. We further observed a synergistic effect of the intermix of the phytonutrients on ALP activity. Intermixes of phytonutrients may serve as convenient and effective nutritional countermeasures against bone loss in space.

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Shared Genetic and Epigenetic Mechanisms between the Osteogenic Differentiation of Dental Pulp Stem Cells and Bone Marrow Stem Cells

Cited by 19 publications

References 100 publications

Naringin promotes osteogenesis and ameliorates osteoporosis development by targeting JAK2/STAT3 signalling

Naringin promotes osteogenesis and ameliorates osteoporosis development by targeting JAK2/STAT3 signalling

Methylsulfonylmethane enhances MSC chondrogenic commitment and promotes pre-osteoblasts formation

Nutraceuticals Synergistically Promote Osteogenesis in Cultured 7F2 Osteoblasts and Mitigate Inhibition of Differentiation and Maturation in Simulated Microgravity

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