Shared-probe system: An accurate, low-cost and general enzyme-assisted DNA probe system for detection of genetic mutation

Detection of Single Nucleotide Polymorphisms of Circulating Tumor DNA by Strand Displacement Amplification Coupled with Liquid Chromatography

“…C irculating tumor DNA (ctDNA) is a DNA fragment produced by apoptosis, necrosis, or secretion of tumor cells. 1−3 As a tumor biomarker of cancers, it can specifically identify abnormal tumor changes for diagnosis, 4,5 treatment monitoring, 6 and prognosis. 7,8 However, many studies have shown that the analysis of one type of ctDNA is not sufficient to provide reliable information to diagnose a specific disease because disorders of one type of ctDNA may be associated with a number of diseases.…”

“…Circulating tumor DNA (ctDNA) is a DNA fragment produced by apoptosis, necrosis, or secretion of tumor cells. − As a tumor biomarker of cancers, it can specifically identify abnormal tumor changes for diagnosis, , treatment monitoring, and prognosis. , However, many studies have shown that the analysis of one type of ctDNA is not sufficient to provide reliable information to diagnose a specific disease because disorders of one type of ctDNA may be associated with a number of diseases. , For example, the proto-oncogene KRAS is found to be upregulated in colorectal cancer, , lung cancer, pancreatic cancer, , and other tumors. , Alternatively, the progression of one kind of disease may be associated with several types of ctDNAs. For example, PIK3CA, TP53, CDH1, and GATA3 are maladjusted at all stages in breast cancer patients .…”