2011
DOI: 10.1038/nmeth.1624
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Sharper low-power STED nanoscopy by time gating

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Cited by 400 publications
(404 citation statements)
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“…Application of ExPAN notably reduced the duration for which the molecules emitted fluorescence. The intensity of the stimulated emission beam was ~1.7 MW/cm 2 for all ExPAN experiments shown here 18 . In principle, the duration for which a single molecule fluoresces can be reduced as desired by changing the power of the stimulated emission beam (see Online Methods), but the intensities described above usually sufficed because the demodulation approach does not require a complete on-and-off behavior of the signals from individual emitters.…”
Section: Validation Using Single Molecules and Labeled Nanospheresmentioning
confidence: 90%
“…Application of ExPAN notably reduced the duration for which the molecules emitted fluorescence. The intensity of the stimulated emission beam was ~1.7 MW/cm 2 for all ExPAN experiments shown here 18 . In principle, the duration for which a single molecule fluoresces can be reduced as desired by changing the power of the stimulated emission beam (see Online Methods), but the intensities described above usually sufficed because the demodulation approach does not require a complete on-and-off behavior of the signals from individual emitters.…”
Section: Validation Using Single Molecules and Labeled Nanospheresmentioning
confidence: 90%
“…The STED cross sections were fit to a Lorentzian, for which there is precedent in the literature. 15,21 The fwhms were 49 and 39 nm for the left and right bead, respectively. Table 1 shows the fwhm obtained by fitting the X-and Yfluorescence intensity cross sections of 15 beads with confocal or STED illumination.…”
Section: ■ Materials and Methodsmentioning
confidence: 98%
“…Likewise, RESOLFT based on photoswitching fluorescent proteins saturates the molecular transition that deactivates the FP in order to restrict the active region to subdiffraction dimensions pulsed excitation with a CW-depletion beam and timecorrelated single photon counting detection (gated STED or g-STED) solves this problem allowing synchronicity between excitation and detection. g-STED has been demonstrated to provide the same resolution with ten times lower depletion intensities (≈0.1 GW cm −2 ) than pulsed STED [12,13].…”
Section: Stedmentioning
confidence: 99%