Shear horizontal surface acoustic wave microsensor for Class A viral and bacterial detection.

Branch, Darren W.; Huber, Dale L.; Brozik, Susan M.; Edwards, Thayne L.

doi:10.2172/1028915

Cited by 6 publications

(9 citation statements)

References 36 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…[30] Although quar has lower electro-mechanical coupling than some sensors made from other materials, such as lithium niobite and lithium tantalate, quar has the unique advantage of having a near-zero temperature coefficient. [31] Therefore, quar biosensors are not affected as severely by thermal drift and high coefficients of variances [30]. Therefore, these devices can be used without significant insulation around them or without having to use thermal compensation to correct the data.…”

Section: Methodsmentioning

confidence: 99%

Evaluation of the Ability of Using Shear-horizontal Surface Acoustic Wave Biosensors to Perform Sterility Testing of Nutrient Media

Aimofumhe,

Badewole,

Smith

et al. 2024

Preprint

View full text Add to dashboard Cite

Abstracts In this article, we describe a simple procedure for evaluating the sterility of nutrient media using a shear-horizontal surface acoustic wave (SH-SAW) biosensor. We used the SH-SAW biosensor platform to monitor the time-dependent phase responses of the material for the media that binds to a monolayer of 3-mercaptopropanyl-N-hydroxy succinimide on the biosensor surface. Increases in the phase shift less than 50% of the starting phase shift recorded 180 minutes after the sterilization indicate a successful sterility test of the media. Our devices were then applied to sterility testing using a viable Escherichia coli (E. coli) K-12 solution to confirm our hypothesis. Filtered LB Broth was the negative control, and a 24-hour culture of E. coli was the positive control. Before testing, the cell solution was pelleted, the media decanted, and then replaced with a 1x phosphate-buffered saline solution (1x PBS). Since we know that the N-hydroxysuccinimide residues will bind to amine groups, only in cases where microbial species are present will the surface binding increase greater than 50%. We confirmed our hypothesis using SH-SAW biosensors and overnight E. coli cultures. The sensitivity for our 250 MHz quartz SH-SAW sensors was calculated based on the response from the E. coli K-12 solution over the linear dynamic range, which appears to be 2x10^4 – 2x10^7 CFU per ml and has a sensitivity of S = 1.655x10^5 o m2 Kg-1. The limit of detection (LOD) for these sensors was calculated to be approximately 2x10^-9 grams (g), which is estimated to be approximately 2,000 Escherichia coli (E. coli) K-12 cells. However, the sensitivity and insertion loss remained approximately unchanged before and after coating the sensor with 3,3'-Dithiobis(succinimidyl) Propionate (DSP).

show abstract

Section: Methodsmentioning

confidence: 99%

Evaluation of the Ability of Using Shear-horizontal Surface Acoustic Wave Biosensors to Perform Sterility Testing of Nutrient Media

Aimofumhe,

Badewole,

Smith

et al. 2024

Preprint

View full text Add to dashboard Cite

show abstract

“…[30] Although quartz has lower electro-mechanical coupling than some sensors made from other materials, such as lithium niobite and lithium tantalate, quartz has the unique advantage of having a near-zero temperature coefficient. [31] Therefore, quartz biosensors are not affected as severely by thermal drift and high coefficients of variances [30]. Therefore, these devices can be used without significant insulation around them or without having to use thermal compensation to correct the data.…”

Section: Methodsmentioning

confidence: 99%

Evaluation of the Ability of Using Shear-horizontal Surface Acoustic Wave Biosensors to Perform Sterility Testing of Nutrient Media

Sigmus,

Badewole,

Smith

et al. 2024

Preprint

View full text Add to dashboard Cite

In this article, we describe a simple procedure for evaluating the sterility of nutrient media using a shear-horizontal surface acoustic wave (SH-SAW) biosensor. We used the SH-SAW biosensor platform to monitor the time-dependent phase responses of the material for the media that binds to a monolayer of 3-mercaptopropanyl-N-hydroxy succinimide on the biosensor surface. Increases in the phase shift less than 50% of the starting phase shift recorded 180 minutes after the sterilization indicate a successful sterility test of the media. Our devices were then applied to sterility testing using a viable Escherichia coli (E. coli) K-12 solution to confirm our hypothesis. Filtered LB Broth was the negative control, and a 24-hour culture of E. coli was the positive control. Before testing, the cell solution was pelleted, the media decanted and then replaced with a 1x phosphate-buffered saline solution (1x PBS). Since we know that the N-hydroxysuccinimide residues will bind to amine groups, only in cases where microbial species are present will the surface binding increase greater than 50%. We confirmed our hypothesis using SH-SAW biosensors and overnight E. coli cultures. The sensitivity for our 250 MHz quartz SH-SAW sensors was calculated based on the response from the E. coli K-12 solution over the linear dynamic range, which appears to be 2x104 – 2x107 CFU per ml and has a sensitivity of S = 1.655x105 o m2 Kg-1. The limit of detection (LOD) for these sensors was calculated to be approximately 2x10-9 grams (g) which is estimated to be approximately 2,000 Escherichia coli (E. coli) K-12 cells. However, the sensitivity and insertion loss remained approximately unchanged before and after coating the sensor with 3,3'-Dithiobis(succinimidyl) Propionate (DSP).

show abstract

“…[333][334][335][336]). The former can typically be oscillated at frequencies of ~5-10 MHz, while the latter can achieve resonances >300 MHz.…”

Section: High-frequency Mechanical Resonatorsmentioning

confidence: 99%

Studies of the viscoelastic properties of water confined between surfaces of specified chemical nature.

Houston¹,

Grest²,

Moore³

et al. 2010

View full text Add to dashboard Cite

This report summarizes the work completed under the Laboratory Directed Research and Development (LDRD) project 10-0973 of the same title. Understanding the molecular origin of the no-slip boundary condition remains vitally important for understanding molecular transport in biological, environmental and energy-related processes, with broad technological implications. Moreover, the viscoelastic properties of fluids in nanoconfinement or near surfaces are not well-understood. We have critically reviewed progress in this area, evaluated key experimental and theoretical methods, and made unique and important discoveries addressing these and related scientific questions. Thematically, the discoveries include insight into the orientation of water molecules on metal surfaces, the premelting of ice, the nucleation of water and alcohol vapors between surface asperities and the lubricity of these molecules when confined inside nanopores, the influence of water nucleation on adhesion to salts and silicates, and the growth and superplasticity of NaCl nanowires.

show abstract

Shear horizontal surface acoustic wave microsensor for Class A viral and bacterial detection.

Cited by 6 publications

References 36 publications

Evaluation of the Ability of Using Shear-horizontal Surface Acoustic Wave Biosensors to Perform Sterility Testing of Nutrient Media

Evaluation of the Ability of Using Shear-horizontal Surface Acoustic Wave Biosensors to Perform Sterility Testing of Nutrient Media

Evaluation of the Ability of Using Shear-horizontal Surface Acoustic Wave Biosensors to Perform Sterility Testing of Nutrient Media

Studies of the viscoelastic properties of water confined between surfaces of specified chemical nature.

Contact Info

Product

Resources

About