Shear-induced aggregation of amyloid β (1–40) in a parallel plate geometry

Sharma, Laipubam Gayatri; Pandey, Lalit M.

doi:10.1080/07391102.2020.1798814

Cited by 17 publications

(12 citation statements)

References 35 publications

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“…Further, the conformation changes of the combined heat and UV treated IgG at the above optimized condition (70 °C for 15 minutes) were analyzed as compared to the native IgG using Fourier Transform Infrared Spectroscopy (FTIR) analysis. The FTIR spectra of native and heat treated IgG are shown in Figure 5 , which were de-convoluted and the area of de-convoluted peaks have been used to deduce the contents of the secondary structure ( Sharma et al, 2020 ). The contents of α-helix, β-sheet and β-turn in native IgG were found to be 32, 55 and 13 %, respectively, which agreed with the reported data ( Hasan et al, 2018 ).…”

Section: Resultsmentioning

confidence: 99%

“…The software resulted in 13 different combinations of input variables (time and temperature). The hydrodynamic sizes obtained through dynamic light scattering (DLS), optical density at 280 nm (OD 280 ) and intrinsic fluorescence were considered as response variables, which reflect the unfolding of the protein ( Sharma & Pandey, 2021 ; Sharma et al, 2020 ).…”

Section: Methodsmentioning

confidence: 99%

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Performance study of a sterilization box using a combination of heat and ultraviolet light irradiation for the prevention of COVID-19

Mahanta

Saxena

Pandey

et al. 2021

Environmental Research

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SARS-CoV-2 virus and other pathogenic microbes are transmitted to the environment through contacting surfaces, which need to be sterilized for the prevention of COVID-19 and related diseases. In this study, a prototype of a cost-effective sterilization box is developed to disinfect small items. The box utilizes ultra violet (UV) radiation with heat. For performance assessment, two studies were performed. First, IgG (glycoprotein, a model protein similar to that of spike glycoprotein of SARS-COV-2) was incubated under UV and heat sterilization. An incubation with UV at 70 °C for 15 minutes was found to be effective in unfolding and aggregation of the protein. At optimized condition, the hydrodynamic size of the protein increased to ∼171 nm from ∼5 nm of the native protein. Similarly, the OD 280 values also increased from 0.17 to 0.78 indicating the exposure of more aromatic moieties and unfolding of the protein. The unfolding and aggregation of the protein were further confirmed by the intrinsic fluorescence measurement and FTIR studies, showing a 70% increase in the β-sheets and a 22 % decrease in the α-helixes of the protein. The designed box was effective in damaging the protein’s native structure indicating the effective inactivation of the SARS-COV-2. Furthermore, the incubation at 70 °C for 15 minutes inside the chamber resulted in 100 % antibacterial efficacy for the clinically relevant E.coli bacteria as well as for bacteria collected from daily use items. It is the first detailed performance study on the efficacy of using UV irradiation and heat together for disinfection from virus and bacteria.

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Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Performance study of a sterilization box using a combination of heat and ultraviolet light irradiation for the prevention of COVID-19

Mahanta

Saxena

Pandey

et al. 2021

Environmental Research

Self Cite

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“…Amyloidogenesis in functional, applied, and disease-related situations is dependent on the dynamics of the microenvironment. − Fluid interfaces (gas–liquid or liquid–liquid) are an important aspect of the protein environment, which have been shown to significantly influence amyloidogenesis through alterations to energetics/hydrophobicity and protein stability, nucleation and elongation, and surface protein concentration. − While the presence and properties of an undisturbed fluid interface affect amyloidogenesis, fluid interfaces in biological systems are often subject to forces, such as fluid shear, which further influence amyloidogenesis kinetics. Fluid shear increases the induction and fibrillization rate of amyloidogenesis in multiple types of amyloid proteins and flow geometries. − Previous research in systems with fluid interfaces also indicates that shear normal to the interface drives amyloidogenesis. , Additionally, shear flow is relevant to biological interfaces, such as those in the brain, and the neurological disorders caused by several of the aforementioned amyloid diseases. Specifically, the flow of cerebrospinal fluid, clearance, or accumulation of amyloid fibrils and their precursors, and circulation of amyloid proteins in the brain are fundamental to understanding amyloidogenesis, associated neurodegenerative diseases, and the development of clinical treatments. − While the impact of shear flow has been documented in many situations, the effect of interfacially driven shear flow on amyloidogenesis remains unexplored.…”

Section: Introductionmentioning

confidence: 99%

Effects of Shear Rate and Protein Concentration on Amyloidogenesis via Interfacial Shear

et al. 2021

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The influence of hydrodynamics on protein fibrillization kinetics is relevant to biophysics, biochemical reactors, medicine, and disease. This investigation focused on the effects of interfacial shear on the fibrillization kinetics of insulin. Human insulin served as a model protein for studying shear-induced fibrillization with relevance to amyloid diseases such as Alzheimer's, Parkinson's, prions, and type 2 diabetes. Insulin solutions at different protein concentrations were subjected to shear flows with prescribed interfacial angular velocities using a knife-edge (surface) viscometer (KEV) operating in a laminar axisymmetric flow regime where inertia is significant. Fibrillization kinetics were quantified using intrinsic fibrillization rate and times (onset, half, and end) determined through spectroscopic measurement of monomer extinction curves and fitting to a sigmoidal function. Additionally, the occurrence of gelation was determined through macroscopic imaging and transient fibril microstructure was captured using fluorescence microscopy. The results showed that increasing interfacial shear rate produced a monotonic increase in intrinsic fibrillization rate and a monotonic decrease in fibrillization time. Protein concentration did not significantly impact the intrinsic fibrillization rate or times; however, a minimum fibril concentration for gelation was found. Protein microstructure showed increasing aggregation and plaque/cluster formation with time.

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“…Despite the consistently cyclical action of blade reciprocation, the unpredictable variability of fluid velocity entering the cutter port due to viscosity changes and blade dragging results in fragments of extremely variable MW. The increased shear rate created at the cutter port by exceedingly high cut rates may create smaller protein aggregates, 16 thus explaining the reduced proportion of proteins of less than 50 kDa ( Fig. 2 ).…”

Section: Discussionmentioning

confidence: 99%

Human Vitreous Collagen Fragments Dimension As a Function of Vitrectomy Cut Rate

Rossi

Speciale

Menichini

et al. 2022

Trans. Vis. Sci. Tech.

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Purpose To study the dimensions and distribution of human vitreous collagen type II fragments collected after vitrectomy performed at varying cut rates and to evaluate if increasing the cut rate produces smaller collagen fragments, thus reducing retinal traction and/or viscosity. Methods Fluid was collected during core vitrectomies performed for macular surgery at cut rates from 1000 to 16,000 cuts per minute (CPM) and immediately refrigerated. Protein fractions were separated by molecular weight (MW; >100 kDa, 50–100 kDa, 50–30 kDa, 30–10 kDa, and <10 kDa) through centrifugal filters. The Human Collagen II ELISA Kit colorimetric assay was then used to measure the COL2A1 in unfiltered and filtered samples. Results Vitreous samples collected after vitrectomy performed at 16,000 CPM contained a higher concentration of protein with MW over 100 kDa than at any other cutting frequency ( P < 0.01). No significant differences were found in fractions collected with a MW between 50 and 100 kDa. Collagen type II fragments over 100 kDa were significantly more represented than smaller fragments at each cut rate. The proportion of smaller (50–100 kDa) collagen fragments compared with those over 100 kDa was higher at 2000 CPM than at higher cut rates. Conclusions Vitreous samples collected at different cut rates do not contain a significantly different proportion of collagen type II fragments of the tested MW. The extreme variability of vitreous flow through the cutter port may explain the uncertain predictability of collagen fragment MWs. Translational Relevance Increasing the cut rate does not produce vitreous fragments of proportionally smaller dimension. It is necessary to achieve an invariant instantaneous flow through the cutter port in order to decrease retinal traction during vitrectomy.

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Shear-induced aggregation of amyloid β (1–40) in a parallel plate geometry

Cited by 17 publications

References 35 publications

Performance study of a sterilization box using a combination of heat and ultraviolet light irradiation for the prevention of COVID-19

Performance study of a sterilization box using a combination of heat and ultraviolet light irradiation for the prevention of COVID-19

Effects of Shear Rate and Protein Concentration on Amyloidogenesis via Interfacial Shear

Human Vitreous Collagen Fragments Dimension As a Function of Vitrectomy Cut Rate

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