In the prestressed composite girder bridges, the shear connectors will transfer a portion of prestress to the steel girders.However, more prestress is preferred to be remained in the concrete slab to improve its anti-cracking behavior and serviceability performance. A Partial Connection-Prestressing (PCP) method is proposed in this paper to decrease the prestress transferred to steel girders. The partial connection is achieved by applying Rubber-Sleeved Studs (RSS) in the prestressed concrete slab in the negative moment region. Since there is no experimental research regarding PCP method in continuous composite girders, two continuous girders specimens were tested under four-point static load to evaluate their mechanical performance. The test results showed that without decreasing ultimate strength and overall stiffness of the steel-concrete composite girders, the PCP method enhanced the cracking load by 3.1 times. Besides, only 12.5% of the prestressing force was transferred into the steel girder at the internal support section with PCP method, while that in girder without PCP reached 25.9%. Thereafter, to elucidate the partial interaction effect and prestress effect on continuous girders' mechanical behavior, numerical models were developed and calibrated with the test results. It was revealed in the numerical analysis that compared with normal composite girder, applying RSS or prestress alone can improve the cracking load respectively by 11.8% and 157.0 %, while the figure increases to 234.3% when applying RSS and prestressing at the same time. The results indicate that applying RSS in the negative moment region can effectively increase the prestressing efficiency in the concrete slab as well as enhance the bridge serviceability and durability.