Shear stress in the intervillous space promotes trophoblast syncytium formation.

Inohaya, Asako; Chigusa, Yoshitsugu; Matsuzaka, Yu; Yasuda, Eriko; Takakura, Masahito; Ueda, Yusuke; Kawamura, Yosuke; Io, Shingo; Mogami, Haruta; Kondoh, Eiji; Mandai, Masaki

doi:10.1016/j.placenta.2021.08.044

Cited by 1 publication

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“…Therefore, it was reported that shear stress is necessary for the syncytialization of trophoblasts. 69 In this study, we have used COMSOL Multiphysics software to calculate FSS in our microfluidic system (Figure 6a and b). The shear stress along the width of the channel where the cells were seeded was calculated to have a range of 0.02−0.2 dyn/ cm 2 (Figure 6a).…”

Section: Resultsmentioning

confidence: 99%

“…Therefore, it was reported that shear stress is necessary for the syncytialization of trophoblasts. 69 …”

Section: Resultsmentioning

confidence: 99%

“…When trophectoderm-like cells from human naive induced pluripotent stem cells were placed under a shear stress of 10 dyn/cm 2 after differentiation to trophoblast-like cells, the cells subjected to shear stress showed proof of syncytium formation compared to those not subjected to shear stress. Therefore, it was reported that shear stress is necessary for the syncytialization of trophoblasts …”

Section: Resultsmentioning

confidence: 99%

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Transport of Maternally Administered Pharmaceutical Agents Across the Placental Barrier In Vitro

Pemathilaka

Alimoradi

Reynolds

et al. 2022

ACS Appl. Bio Mater.

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To understand the transport of pharmaceutical agents and their effects on developing fetus, we have created a placental microsystem that mimics structural phenotypes and physiological characteristic of a placental barrier. We have shown the formation of a continuous network of epithelial adherens junctions and endothelial cell–cell junctions confirming the integrity of the placental barrier. More importantly, the formation of elongated microvilli under dynamic flow condition is demonstrated. Fluid shear stress acts as a mechanical cue triggering the microvilli formation. Pharmaceutical agents were administered to the maternal channel, and the concentration of pharmaceutical agents in fetal channel for coculture and control models were evaluated. In fetal channel, the coculture model exhibited about 2.5 and 2.2% of the maternal initial concentration for naltrexone and 6β-naltrexol, respectively. In acellular model, fetal channel showed about 10.5 and 10.3% of the maternal initial concentration for naltrexone and 6β-naltrexol, respectively. Gene expressions of epithelial cells after direct administration of naltrexone and 6β-naltrexol to the maternal channel and endothelial cells after exposure due to transport through placental barrier are also reported.

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Section: Resultsmentioning

confidence: 99%

“…Therefore, it was reported that shear stress is necessary for the syncytialization of trophoblasts. 69 …”

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

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