Shear stress induces endothelial cell phenotype in human adipose-derived adult stem cells

Fischer, Leslie M.; Lee, Alice; DiMatteo, Christopher; Golesorkhi, Negar; Wrigley, C.; Galler, Avi S; Tulenko, Thomas N.; DiMuzio, Paul

doi:10.1016/j.jamcollsurg.2006.05.275

Cited by 3 publications

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“…Fluid flow and associated shear forces affect cell growth and differentiation. [40][41][42][43] Interstitial flow also affect the extracellular matrix and growth factors which lead to changes in surrounding tissue. [44][45][46] Accordingly, we simulated both the fluid flow and diffusion profiles through the microvessel manifold.…”

Section: Modelled Delivery Of Nutrients In Manifold Devicementioning

confidence: 99%

Microvessel manifold for perfusion and media exchange in three-dimensional cell cultures

et al. 2016

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Integrating a perfusable microvasculature system is a substantial challenge for "on-chip" tissue models. We have developed an inclusive on-chip platform that is capable of maintaining laminar flow through porous biosynthetic microvessels. The biomimetic microfluidic device is able to deliver and generate a steady perfusion of media containing small-molecule nutrients, drugs, and gases in three-dimensional cell cultures, while replicating flow-induced mechanical stimuli. Here, we characterize the diffusion of small molecules from the perfusate, across the microvessel wall, and into the matrix of a 3D cell culture.

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Section: Modelled Delivery Of Nutrients In Manifold Devicementioning

confidence: 99%

Microvessel manifold for perfusion and media exchange in three-dimensional cell cultures

et al. 2016

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“…Two specific stimuli for differentiation have been examined in vitro: chemical stimuli with vascular endothelial growth factor and other growth factors (unpublished data, 2006) and physical stimuli with shear stress. 21 Prior to stimulation, ASCs are negative for each of the above-noted endothelial characteristics. After culture in differentiating media for up to 3 weeks, the stem cells realign with flow and form cords in response to Matrigel (Figure 3).…”

Section: Cell Choicementioning

confidence: 99%

Development of a Tissue-Engineered Bypass Graft Seeded with Stem Cells

et al. 2006

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The gold standard conduit for bypass of diseased small-diameter arteries remains autologous vascular tissue. In the absence of such tissue, patients are offered bypass with prosthetic material, with far less durable results. Vascular tissue engineering, the creation of a vascular conduit by seeding a tubular scaffold with various cells, may offer an alternative approach to this difficult situation. Herein we review some of the significant challenges that remain in designing an ideal vascular conduit and outline potential solutions offered by a graft created by seeding natural vascular tissue (decellularized vein allograft) with readily available autologous cells (adipose-derived stem cells).

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Tissue engineering applications to vascular bypass graft development: The use of adipose-derived stem cells

DiMuzio

Tulenko

2007

Journal of Vascular Surgery

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The burgeoning field of vascular tissue engineering holds promise for the creation of a practical and successful small-diameter arterial bypass graft. Many creative combinations of autologous cells and scaffolds exist along with an equally long list of microenvironmental cues used to create a functional arterial conduit. This review outlines our work using abdominal wall fat as a source of autologous stem cells for vascular tissue engineering, focusing specifically on this stem cell's availability and potency to differentiate into endothelial-like cells. In a series of 49 patients undergoing elective peripheral vascular surgery, an abundant quantity of adult stem cells was harvested from fat obtained by liposuction. The efficacy of the isolation did not appear influenced by advanced age, obesity, renal failure, or vascular disease, although fat from diabetic patients yielded significantly less stem cells. In addition, these adipose-derived stem cells acquired several morphologic and molecular endothelial phenotypes when exposed to growth factors (endothelial cell growth supplement and vascular endothelial growth factor) and physiologic shear stress in vitro. Taken together, these studies suggest that fat appears to be a viable source of autologous stem cells for use in vascular tissue engineering.

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Shear stress induces endothelial cell phenotype in human adipose-derived adult stem cells

Cited by 3 publications

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Microvessel manifold for perfusion and media exchange in three-dimensional cell cultures

Microvessel manifold for perfusion and media exchange in three-dimensional cell cultures

Development of a Tissue-Engineered Bypass Graft Seeded with Stem Cells

Tissue engineering applications to vascular bypass graft development: The use of adipose-derived stem cells

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