Shed membrane vesicles and clustering of membrane-bound proteolytic enzymes

Vittorelli, Maria Letizia

doi:10.1016/s0070-2153(03)54017-0

Cited by 22 publications

(23 citation statements)

References 64 publications

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“…There are many reports that highlight a potential role for growth factors in cell activation and subsequent microvesicle release, because removal of serum from the growth medium abolishes microvesicle release (Vittorelli, 2003). There is also substantial evidence of increased shedding activity in microglia and dendritic cells when stimulated with Ca 2+ (Cocucci et al, 2009).…”

Section: Impact Of the Cytoskeletonmentioning

confidence: 99%

Microvesicles: mediators of extracellular communication during cancer progression

Muralidharan‐Chari

Clancy

Sedgwick

et al. 2010

Journal of Cell Science

831

765

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SummaryMicrovesicles are generated by the outward budding and fission of membrane vesicles from the cell surface. Recent studies suggest that microvesicle shedding is a highly regulated process that occurs in a spectrum of cell types and, more frequently, in tumor cells. Microvesicles have been widely detected in various biological fluids including peripheral blood, urine and ascitic fluids, and their function and composition depend on the cells from which they originate. By facilitating the horizontal transfer of bioactive molecules such as proteins, RNAs and microRNAs, they are now thought to have vital roles in tumor invasion and metastases, inflammation, coagulation, and stem-cell renewal and expansion. This Commentary summarizes recent literature on the properties and biogenesis of microvesicles and their potential role in cancer progression.

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Section: Impact Of the Cytoskeletonmentioning

confidence: 99%

Microvesicles: mediators of extracellular communication during cancer progression

Muralidharan‐Chari

Clancy

Sedgwick

et al. 2010

Journal of Cell Science

831

765

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“…Tumor cells have also been shown to acquire proteins associated with vesicles, a passive process by which neoplastic cells take up proteins associated with the plasma membrane (60). Examples of proteins shed by vesicles include matrix-degrading proteinases (59,61), cathepsins B and D (62-65), BRCA1 (66), IL-1␤ (67), fibroblast growth factor-2 (68), and tumor-associated surface antigens (69). Using protein tagging of whole cells, Jang and Hanash recently identified several proteins in the cell surface of leukemia cells that have previously been shown to occur only in the endoplasmic reticulum (70).…”

mentioning

confidence: 99%

Proteomic Characterization of the Interstitial Fluid Perfusing the Breast Tumor Microenvironment

Celis

Gromov

Cabezón

et al. 2004

Molecular & Cellular Proteomics

295

265

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Clinical cancer proteomics aims at the identification of markers for early detection and predictive purposes, as well as to provide novel targets for drug discovery and therapeutic intervention. Proteomics-based analysis of traditional sources of biomarkers, such as serum, plasma, or tissue lyzates, has resulted in a wealth of information and the finding of several potential tumor biomarkers. However, many of these markers have shown limited usefulness in a clinical setting, underscoring the need for new clinically relevant sources. Here we present a novel and highly promising source of biomarkers, the tumor interstitial fluid (TIF) that perfuses the breast tumor microenvironment. We collected TIFs from small pieces of freshly dissected invasive breast carcinomas and analyzed them by two-dimensional polyacrylamide gel electrophoresis in combination with matrix-assisted laser desorption/ionization time-of-flight mass spectrometry, Western immunoblotting, as well as by cytokine-specific antibody arrays. This approach provided for the first time a snapshot of the protein components of the TIF, which we show consists of more than one thousand proteins-either secreted, shed by membrane vesicles, or externalized due to cell deathproduced by the complex network of cell types that make up the tumor microenvironment. So far, we have identified 267 primary translation products including, but not limited to, proteins involved in cell proliferation, invasion, angiogenesis, metastasis, inflammation, protein synthesis, energy metabolism, oxidative stress, the actin cytoskeleton assembly, protein folding, and transport. As expected, the TIF contained several classical serum proteins. Considering that the protein composition of the TIF reflects the physiological and pathological state of the tissue, it should provide a new and potentially rich resource for diagnostic biomarker discovery and for identifying more selective targets for therapeutic intervention. Molecular & Cellular Proteomics 3:327-344, 2004.

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“…11 Their diameter ranges between ϳ0.1 and 1 m, and their function has been associated to the function of the cell from which they originate. 12,13 Many types of cells release exovesicles, but so far little is known on those originating from DCs. On the other hand, the release of exosomes by DCs has been established.…”

mentioning

confidence: 99%

Exovesicles from Human Activated Dendritic Cells Fuse with Resting Dendritic Cells, Allowing Them to Present Alloantigens

Obregon

Rothen‐Rutishauser

Kiama

et al. 2006

The American Journal of Pathology

100

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Dendritic cells (DCs) can release microvesicles, but the latter's numbers, size, and fate are unclear. Fluorescently labeled DCs were visualized by laser-scanning microscopy. Using a Surpass algorithm, we were able to identify and quantify per cell several hundred microvesicles released from the surface of stimulated DCs. We show that most of these microvesicles are not of endocytic origin but result from budding of the plasma membrane, hence their name, exovesicle. Using a double vital staining, we show that exovesicles isolated from activated DCs can fuse with the membrane of resting DCs, thereby allowing them to present alloantigens to lymphocytes. We concluded that, within a few hours from their release, exovesicles may amplify local or distant adaptive immunological response.

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Shed membrane vesicles and clustering of membrane-bound proteolytic enzymes

Cited by 22 publications

References 64 publications

Microvesicles: mediators of extracellular communication during cancer progression

Microvesicles: mediators of extracellular communication during cancer progression

Proteomic Characterization of the Interstitial Fluid Perfusing the Breast Tumor Microenvironment

Exovesicles from Human Activated Dendritic Cells Fuse with Resting Dendritic Cells, Allowing Them to Present Alloantigens

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