Shedding light on ONOO<sup>−</sup> detection: the emergence of a fast-response fluorescent probe for biological systems

Wu, Guoliang; Li, Zihong; Huang, Ping; Lin, Weiying

doi:10.1039/d3tb02994h

J. Mater. Chem. B

2024

DOI: 10.1039/d3tb02994h

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Shedding light on ONOO⁻ detection: the emergence of a fast-response fluorescent probe for biological systems

Guoliang Wu,

Zihong Li,

Ping Huang

et al.

Abstract: ONOO-, a bioactive molecule, plays a critical role in inflammation-related signaling pathways and pathological mechanisms. Numerous studies have established a direct correlation between elevated ONOO- levels and tumor progression. Therefore,...

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Cited by 3 publications

References 40 publications

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Development of a hybrid rhodamine-hydrazine NIR fluorescent probe for sensitive detection and imaging of peroxynitrite in necrotizing enterocolitis model

Zhang,

Hu,

Rong

et al. 2024

Bioorganic Chemistry

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Development of a hybrid rhodamine-hydrazine NIR fluorescent probe for sensitive detection and imaging of peroxynitrite in necrotizing enterocolitis model

Zhang,

Hu,

Rong

et al. 2024

Bioorganic Chemistry

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An innovative fluorescent probe for monitoring of ONOO− in multiple liver-injury models

Fan,

Ma,

Chi

et al. 2025

Talanta

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Multifunctional Sensors for Successive Detection of Endogenous ONOO^– and Mitochondrial Viscosity: Discriminating Normal to Cancer Models

Shen,

Rajalakshmi,

Muthusamy

et al. 2024

Anal. Chem.

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Diagnosing cancer in its early stages can play an important role in prolonging the lifespan of patients, which demands the use of powerful tools to detect biomarkers accurately. However, since most fluorescent probes described for cancer diagnosis are limited to recognizing a single molecule, achieving the high accuracy criteria remains difficult. Here, sensor 1 is constructed for the sequential detection of D, ONOO − , and viscosity. Initially, sensor 1 detected D and underwent an intramolecular charge transfer mechanism, resulting in the formation of 2 and fluorescence quenching at 587 nm. Subsequently, the intermediate (2) monitored ONOO − and reproduced sensor 1 reversibly with fluorescence enhancement at 496 nm, showing concentration-related quantitative analysis. Similar sensing processes were observed in monitoring ONOO − and viscosity by synthetically developed sensor 2. The proposed mechanisms of sensors 1 and 2 are verified through various characterizations ( 1 H NMR, HR-MS, and HPLC) and DFT calculations. Investigations on endogenous ONOO − and mitochondrial viscosity in cancer (HeLa) and normal (NCM460) cells were conducted to distinguish cancerous cells from normal cells. We anticipated that sensor 2 could effectively serve as a reliable bioanalytical reagent for cancer diagnosis at an earlier stage through sequential detection of two cancer markers, ONOO − and mitochondrial viscosity, in living cells. Importantly, sensor 2 has been employed for imaging ONOO − in normal and liver injury mouse models and tissues, achieving outstanding results.

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Shedding light on ONOO⁻ detection: the emergence of a fast-response fluorescent probe for biological systems

Abstract: ONOO-, a bioactive molecule, plays a critical role in inflammation-related signaling pathways and pathological mechanisms. Numerous studies have established a direct correlation between elevated ONOO- levels and tumor progression. Therefore,...

Cited by 3 publications

References 40 publications

Development of a hybrid rhodamine-hydrazine NIR fluorescent probe for sensitive detection and imaging of peroxynitrite in necrotizing enterocolitis model

Development of a hybrid rhodamine-hydrazine NIR fluorescent probe for sensitive detection and imaging of peroxynitrite in necrotizing enterocolitis model

An innovative fluorescent probe for monitoring of ONOO− in multiple liver-injury models

Multifunctional Sensors for Successive Detection of Endogenous ONOO^– and Mitochondrial Viscosity: Discriminating Normal to Cancer Models

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Resources

About

Shedding light on ONOO− detection: the emergence of a fast-response fluorescent probe for biological systems

Abstract: ONOO-, a bioactive molecule, plays a critical role in inflammation-related signaling pathways and pathological mechanisms. Numerous studies have established a direct correlation between elevated ONOO- levels and tumor progression. Therefore,...

Cited by 3 publications

References 40 publications

Development of a hybrid rhodamine-hydrazine NIR fluorescent probe for sensitive detection and imaging of peroxynitrite in necrotizing enterocolitis model

Development of a hybrid rhodamine-hydrazine NIR fluorescent probe for sensitive detection and imaging of peroxynitrite in necrotizing enterocolitis model

An innovative fluorescent probe for monitoring of ONOO− in multiple liver-injury models

Multifunctional Sensors for Successive Detection of Endogenous ONOO– and Mitochondrial Viscosity: Discriminating Normal to Cancer Models

Contact Info

Product

Resources

About

Shedding light on ONOO⁻ detection: the emergence of a fast-response fluorescent probe for biological systems

Multifunctional Sensors for Successive Detection of Endogenous ONOO^– and Mitochondrial Viscosity: Discriminating Normal to Cancer Models