Shedding light on proteins, nucleic acids, cells, humans and fish

Setlow, R. B.

doi:10.1016/s1383-5742(02)00004-2

Cited by 21 publications

(11 citation statements)

References 105 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The differences in the inactivation profiles of prions and viruses provided the first clues that the scrapie agent was not a slow virus as had been widely thought (6,23,41,48,58,59,80).…”

Section: Discussionmentioning

confidence: 95%

“…In such cases, the calculation of VOL. 80,2006 PRION INACTIVATION BY ACIDIC SDS 323 a mean underestimates the incubation period because mice that do not become ill are excluded. Applying survival analysis methods overcomes these issues and also incorporates previously censored data on animals that die without showing clinical signs of prion disease (asymptomatic on the last inspection).…”

Section: Inocula Scjd Was Confirmed By Histopathology Immunohistochmentioning

confidence: 99%

See 1 more Smart Citation

Inactivation of Prions by Acidic Sodium Dodecyl Sulfate

Peretz

Supattapone

Giles

et al. 2006

J Virol

117

131

View full text Add to dashboard Cite

. 75:3453-3461, 2001), we investigated the inactivation of prions by sodium dodecyl sulfate (SDS) in weak acid. As judged by sensitivity to proteolytic digestion, the diseasecausing prion protein (PrP Sc ) was denatured at room temperature by SDS at pH values of <4.5 or >10. Exposure of Sc237 prions in Syrian hamster brain homogenates to 1% SDS and 0.5% AcOH at room temperature resulted in a reduction of prion titer by a factor of ca. 10 7 ; however, all of the bioassay hamsters eventually developed prion disease. When various concentrations of SDS and AcOH were tested, the duration and temperature of exposure acted synergistically to inactivate both hamster Sc237 prions and human sporadic Creutzfeldt-Jakob disease (sCJD) prions. The inactivation of prions in brain homogenates and those bound to stainless steel wires was evaluated by using bioassays in transgenic mice. sCJD prions were more than 100,000 times more resistant to inactivation than Sc237 prions, demonstrating that inactivation procedures validated on rodent prions cannot be extrapolated to inactivation of human prions. Some procedures that significantly reduced prion titers in brain homogenates had a limited effect on prions bound to the surface of stainless steel wires. Using acidic SDS combined with autoclaving for 15 min, human sCJD prions bound to stainless steel wires were eliminated. Our findings form the basis for a noncorrosive system that is suitable for inactivating prions on surgical instruments, as well as on other medical and dental equipment.

show abstract

“…The differences in the inactivation profiles of prions and viruses provided the first clues that the scrapie agent was not a slow virus as had been widely thought (6,23,41,48,58,59,80).…”

Section: Discussionmentioning

confidence: 95%

Section: Inocula Scjd Was Confirmed By Histopathology Immunohistochmentioning

confidence: 99%

Inactivation of Prions by Acidic Sodium Dodecyl Sulfate

Peretz

Supattapone

Giles

et al. 2006

J Virol

117

131

View full text Add to dashboard Cite

show abstract

“…UVGI targets the nucleic acids of the bacteria (Setlow, 2002). This study showed that structural and metabolic changes detected by MC and MA play an insignificant part in protecting and restoring the bacteria from UVGI exposure.…”

Section: Cell Damage By Nebulization and Aerosolization And The Effementioning

confidence: 80%

Using selective media to assess aerosolization damage and ultraviolet germicidal irradiation susceptibility of Serratia marcescens

Lai

2005

Aerobiologia

View full text Add to dashboard Cite

This study determined whether selective media, McConkey agar (MC) and minimal salt glucose agar (MA) are suitable for monitoring aerosolization damage of airborne Serratia marcescens in our laboratory aerosol exposure system and assessed the relationship between bacterial culturability in these media and ultraviolet germicidal irradiation (UVGI) susceptibility of the bacteria. Two types of bacterial cultures were prepared. The first culture was taken from bacteria growing on Tryptic soy agar (TSA) as complete medium (fresh culture), which provided nearly 100% of MC/MA tolerant bacteria, while the second one was prepared from freezing the fresh culture (frozen culture), which produced 55 and 81% of MC and MA tolerant bacteria, respectively. We monitored bacterial culturability in TSA, MC and MA from these cultures in the nebulizer reservoir and bioaerosls collected on a six-stage Andersen cascade bio-impactor. The results indicated that both concentration and percentage of MC/MA tolerant bacteria maintained at a similar level during nebulization. For the bioaerosols, although the concentration recovered in the media from the fresh culture was higher than that from the frozen culture, the percentage of MC/MA tolerant bacteria was similar to that before aerosolization. We concluded that MC and MA are not suitable for monitoring aerosolization damage of the bacteria. Moreover, culturability of the bacteria in MC and MA has no effect on their survival after aerosolization. With respect to the bacterial susceptibility to UVGI, MC/MA sensitive and tolerant population as well as the fresh and frozen cultures showed the same susceptibility.

show abstract

“…Photons absorbed by cystine had a higher chance of inactivating a protein than photons absorbed in the aromatic amino acids. The absorbed photons ionize the protein (Setlow, 2002). Aromatic amino acids (e.g., tyrosine and phenylalanine) can absorb UV radiation and recombine to form covalent cross-links in proteins (Gennadios et al, 1998).…”

Section: Nonthermal Methods 621 Pulsed Ultraviolet Lightmentioning

confidence: 99%