Sheep and goats are reservoirs of colistin resistant <i>Escherichia coli</i> that co‐resist critically important antimicrobials: First study from Jordan

Obaidat, Mohammad M.; Tarazi, Yaser H.; AlSmadi, Walaa  M.

doi:10.1111/jfs.13023

Cited by 5 publications

(3 citation statements)

References 46 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Imipenem, classified as a carbapenem, plays a crucial role as a last-resort antibiotic, particularly effective against microorganisms capable of producing extended-spectrum b-lactamases. Instances of carbapenem resistance have been documented in livestock, including goats and sheep in Southeast Asia, and Jordan (Khalifa et al, 2021;Obaidat et al, 2023). The resistance to imipenem raises significant concerns in healthcare settings, limiting the efficacy of this vital antibiotic in treating serious infections.…”

Section: Discussionmentioning

confidence: 99%

Antibiogram profiles of pathogenic and commensal bacteria in goat and sheep feces on smallholder farm

Basnet,

Kilonzo-Nthenge

2024

Front. Antibiot.

View full text Add to dashboard Cite

IntroductionThe increase of antimicrobial resistance (AMR) in zoonotic pathogens poses a substantial threat to both animal production and human health. Although large-scale animal farms are acknowledged as major reservoirs for AMR, there is a notable knowledge gap concerning AMR in small-scale farms. This study seeks to address this gap by collecting and analyzing 137 fecal samples from goat and sheep farms in Tennessee and Georgia.MethodBacteria were identified using culture-dependent methods and polymerase chain reaction (PCR), and antimicrobial susceptibility testing (AST) was performed using the Kirby-Bauer Disk Diffusion method.Results and discussionThe prevalence of E. coli (94.9%) in goats and sheep significantly exceeded (p < 0.05) that of S. aureus (81.0%), Shigella (35.0%), S. saprophyticus, and Salmonella (3.0%). Salmonella occurrence in goat feces (2.2%) was higher than in sheep (0.8%). Notably, 27% of goats and 8% of sheep tested positive for Shigella spp., while 60% of goats and 21% of sheep tested positive for S. aureus. Antibiotic resistance was observed primarily against ampicillin (79.4%), vancomycin (65.1%), and gentamycin (63.6%), significantly surpassing (p < 0.05) resistance to tetracycline (41.6%) and imipenem (21.8%). The penicillin (79.4%), glycopeptide (65.1%), and aminoglycoside (63.6%) antibiotic classes displayed significantly higher (p < 0.05) resistance compared to tetracyclines (45.7%) and carbapenem (21.8%). Our findings suggest that goats and sheep feces may serve as source for multidrug-resistant bacteria, raising concerns about the potential introduction of their fecal matter into soil, water, and eventually to the food chain. This highlights the need for proactive measures to address and mitigate AMR in goats and sheep within small-scale farms.

show abstract

Section: Discussionmentioning

confidence: 99%

Antibiogram profiles of pathogenic and commensal bacteria in goat and sheep feces on smallholder farm

Basnet,

Kilonzo-Nthenge

2024

Front. Antibiot.

View full text Add to dashboard Cite

show abstract

“…Among extended-spectrum β-lactamases (ESBLs) producing Gram-negative bacteria, E. coli is considered the most abundant in host environments representing both an indicator pathogen and a predominant vehicle for resistance transmission in one health context [84,85]. A study published from Jordan in 2022 by Obadiat et al reported small ruminants as reservoirs of colistin-resistant and mcr-1-positive E. coli that exhibit coresistance to critically important antimicrobials [86].…”

Section: Escherichia Colimentioning

confidence: 99%

“…Campylobacter spp., primarily C. jejuni and C. coli, inhabit the gastrointestinal tract of mammals, including goats, sheep, and chickens [86]. Specifically, the foodborne pathogen Campylobacter jejuni, a leading cause of foodborne enteritis, is estimated to account for between 400 and 500 cases of gastroenteritis every year.…”

Section: Campylobacter Sppmentioning

confidence: 99%

Antimicrobial Effects of Plant-Based Supplements on Gut Microbial Diversity in Small Ruminants

Daniel,

Njue,

Sanad

2023

Pathogens

View full text Add to dashboard Cite

Every year in the United States, approximately 48 million people are affected by bacterial illnesses that are transmitted through food, leading to 3000 fatalities. These illnesses typically stem from food animals and their by-products, which may harbor dangerous pathogens like Salmonella enterica, Listeria monocytogenes, enterohemorrhagic Escherichia coli O157:H7, and Campylobacter jejuni. Factors that contribute to contamination include manure used as a soil amendment, exposure to polluted irrigation water, and contact with animals. To improve food safety, researchers are studying pre-slaughter intervention methods to eliminate bacterial contamination in live animals. While small ruminants are vital to global agriculture and income generation for small farms, traditional feeding practices involve supplements and antibiotics to boost performance, which contributes to antibiotic resistance. Hence, researchers are looking for friendly bacterial strains that enhance both animal and human health without impacting livestock productivity. The global trend is to minimize the use of antibiotics as feed supplements, with many countries prohibiting or limiting their use. The aim of this review is to provide a comprehensive insight on the antioxidant capabilities, therapeutic attributes, and applications of bioactive compounds derived from sweet potato tops (SPTs), rice bran (RB) and radish tops (RTs). This overview provides an insight on plant parts that are abundant in antioxidant and prebiotic effects and could be used as value-added products in animal feed and pharmaceutical applications. This review was based on previous findings that supplementation of basal diets with natural supplements represents a multifaceted intervention that will become highly important over time. By remarkably reducing the burden of foodborne pathogens, they apply to multiple species, are cheap, do not require withdrawal periods, and can be applied at any time in food animal production.

show abstract

Conventionally Reared Wallon Meat Lambs Carry Transiently Multi-Drug-Resistant Escherichia coli with Reduced Sensitivity to Colistin Before Slaughter

Dragon,

Jansen,

Dumont

et al. 2024

Animals

View full text Add to dashboard Cite

Major efforts have been made to reduce the use of colistin in livestock since the discovery of the plasmid-borne mobile colistin resistance (mcr) gene in E. coli a decade ago, to curb the burden of its potential transmission to other bacterial species, spread between animals, humans and the environment. This study explored the longitudinal prevalence and characteristics of colistin-resistant and extended-spectrum beta-lactamase-producing (ESBL) E. coli via in vivo fecal and ex vivo carcass swabs from two batches of conventional indoor and organic outdoor Wallon meat sheep from birth to slaughter in 2020 and 2021. Antimicrobial susceptibility testing via broth microdilution revealed that n = 16/109 (15%) E. coli isolates from conventional meat lamb fecal samples had a reduced colistin sensitivity (MIC = 0.5 μg/mL) and thereof, n = 9/109 (8%) were multi-drug-resistant E. coli, while no resistant isolates were recovered from their carcasses. Sequencing revealed causative pmrB genes, indicating that the reduced sensitivity to colistin was not plasmid-borne. While the sample size was small (n = 32), no colistin-resistant and ESBL-producing E. coli were isolated from the organic meat sheep and their carcasses, potentially due to the different husbandry conditions. Prudent and judicious antimicrobial use and strict slaughter hygiene remain imperative for effective risk management to protect consumers in a sustainable One Health approach.

show abstract

Sheep and goats are reservoirs of colistin resistant Escherichia coli that co‐resist critically important antimicrobials: First study from Jordan

Cited by 5 publications

References 46 publications

Antibiogram profiles of pathogenic and commensal bacteria in goat and sheep feces on smallholder farm

Antibiogram profiles of pathogenic and commensal bacteria in goat and sheep feces on smallholder farm

Antimicrobial Effects of Plant-Based Supplements on Gut Microbial Diversity in Small Ruminants

Conventionally Reared Wallon Meat Lambs Carry Transiently Multi-Drug-Resistant Escherichia coli with Reduced Sensitivity to Colistin Before Slaughter

Contact Info

Product

Resources

About