Sheep IGFBP2 and IGFBP4 promoter methylation regulates gene expression and hair follicle development

Tian, Yuezhen; Du, Jianwen; Yang, Xuemei; Zeng, Weidan; He, Junlin; Zhao, Bi; Fu, Xuefeng; Xu, Xinming; Wu, Weiwei; Jiang, Di; Huang, Xixia; Tian, Kechuan

doi:10.1016/j.ejbt.2022.07.001

Cited by 5 publications

(3 citation statements)

References 33 publications

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“…Changes in expression of all igfbp2a, igfbp4, ghri and ghrii after DAC treatment suggest that these genes are modulated, directly or indirectly, by DNA methylation in seabream. Other studies have found evidences of this regulation for igfbp2 in fish (Podgorniak et al, 2019), mice (Kammel et al, 2016) and humans (Boughanem et al, 2021), for igfbp4 in sheep (Tian et al, 2022) and humans (Diaz et al, 2020) and for ghrs in fish (Zhao et al, 2015) and broilers (Cong et al, 2023). Finally, while changes in DNA methylation at the igf1 gene of some fish were reported (Li et al, 2017;Huang et al, 2018), we found no evidence of such regulation in the gilthead seabream as judged by the lack of response of this gene to DAC.…”

Section: Gh-igf-i Axis In Liver Is Modulated In Vitro By Daccontrasting

confidence: 70%

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Section: Gh-igf-i Axis In Liver Is Modulated In Vitro By Daccontrasting

confidence: 70%

Table1.docx

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“…Changes in expression of all igfbp2a, igfbp4, ghri and ghrii after DAC treatment suggest that these genes are modulated, directly or indirectly, by DNA methylation in seabream. Other studies have found evidences of this regulation for igfbp2 in fish ( Podgorniak et al, 2019 ), mice ( Kammel et al, 2016 ) and humans ( Boughanem et al, 2021 ), for igfbp4 in sheep ( Tian et al, 2022 ) and humans ( Diaz et al, 2020 ) and for ghrs in fish ( Zhao et al, 2015 ) and broilers ( Cong et al, 2023 ). Finally, while changes in DNA methylation at the igf1 gene of some fish were reported ( Li et al, 2017 ; Huang et al, 2018 ), we found no evidence of such regulation in the gilthead seabream as judged by the lack of response of this gene to DAC.…”

Section: Discussionmentioning

confidence: 87%

Tissue explants as tools for studying the epigenetic modulation of the GH-IGF-I axis in farmed fish

Perera,

Román-Padilla,

Hidalgo-Pérez

et al. 2024

Front. Physiol.

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Somatic growth in vertebrates is mainly controlled by the growth hormone (GH)/insulin-like growth factor I (IGF-I) axis. The role of epigenetic mechanisms in regulating this axis in fish is far from being understood. This work aimed to optimize and evaluate the use of short-term culture of pituitary and liver explants from a farmed fish, the gilthead seabream Sparus aurata, for studying epigenetic mechanisms involved in GH/IGF-I axis regulation. Our results on viability, structure, proliferation, and functionality of explants support their use in short-term assays. Pituitary explants showed no variation in gh expression after exposure to the DNA methylation inhibitor decitabine (5-Aza-2′-deoxycytidine; DAC), despite responding to DAC by changing dnmt3bb and tet1 expression, and TET activity, producing an increase in overall DNA hydroxymethylation. Conversely, in liver explants, DAC had no effects on dnmts and tets expression or activity, but modified the expression of genes from the GH-IGF-I axis. In particular, the expression of igfbp2a was increased and that of igfbp4, ghri and ghrii was decreased by DAC as well as by genistein, which is suggestive of impaired growth. While incubation of liver explants with S-adenosylmethionine (SAM) produced no clear effects, it is proposed that nutrients must ensure the methylation milieu within the liver in the fish to sustain proper growth, which need further in vivo verification. Pituitary and liver explants from S. aurata can be further used as described herein for the screening of inhibitors or activators of epigenetic regulators, as well as for assessing epigenetic mechanisms behind GH-IGF-I variation in farmed fish.

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“…Table S12. Additional studies of the fine vs. coarse fleece phenotype in sheep [138,333,334,[347][348][349][350][351][352][353][354][355][356][357][358]. Table S13.…”

Section: Supplementary Informationmentioning

confidence: 99%

Genetics of the phenotypic evolution in sheep: a molecular look at diversity-driving genes

et al. 2022

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Background After domestication, the evolution of phenotypically-varied sheep breeds has generated rich biodiversity. This wide phenotypic variation arises as a result of hidden genomic changes that range from a single nucleotide to several thousands of nucleotides. Thus, it is of interest and significance to reveal and understand the genomic changes underlying the phenotypic variation of sheep breeds in order to drive selection towards economically important traits. Review Various traits contribute to the emergence of variation in sheep phenotypic characteristics, including coat color, horns, tail, wool, ears, udder, vertebrae, among others. The genes that determine most of these phenotypic traits have been investigated, which has generated knowledge regarding the genetic determinism of several agriculturally-relevant traits in sheep. In this review, we discuss the genomic knowledge that has emerged in the past few decades regarding the phenotypic traits in sheep, and our ultimate aim is to encourage its practical application in sheep breeding. In addition, in order to expand the current understanding of the sheep genome, we shed light on research gaps that require further investigation. Conclusions Although significant research efforts have been conducted in the past few decades, several aspects of the sheep genome remain unexplored. For the full utilization of the current knowledge of the sheep genome, a wide practical application is still required in order to boost sheep productive performance and contribute to the generation of improved sheep breeds. The accumulated knowledge on the sheep genome will help advance and strengthen sheep breeding programs to face future challenges in the sector, such as climate change, global human population growth, and the increasing demand for products of animal origin.

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Sheep IGFBP2 and IGFBP4 promoter methylation regulates gene expression and hair follicle development

Cited by 5 publications

References 33 publications

Table1.docx

Table1.docx

Tissue explants as tools for studying the epigenetic modulation of the GH-IGF-I axis in farmed fish

Genetics of the phenotypic evolution in sheep: a molecular look at diversity-driving genes

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