Sheep κ-casein peptides inhibit platelet aggregation

Health effects and technological features of caseinomacropeptide

“…This might explain why bovine and human CMP were found at physiologically active concentrations in the plasma of 5-day-old infants and also in adults, although at lower concentrations (Chabance et al, 1995(Chabance et al, , 1998. Furthermore, at least ovine CMP had a very long life when incubated in human or guinea-pig plasma (Qian, Jolle`s, Migliore-Samour, Schoentgen, & Fiat, 1995).…”

“…Bovine, ovine, and caprine CMP can inhibit platelet aggregation and, therefore, the formation of thrombi, because the region 106-116 of k-casein (casoplatelin) is analogous to the fragment 400-411 of fibrinogen g-chain (Jolle`s et al, 1986;Jolle`s, Loucheux-Lefebvre, & Henschen, 1978;Manso, Escudero, Alijo, & Lo´pez-Fandin˜o, 2002;Qian et al, 1995). Thus, CMP from different animal species inhibit fibrinogen binding to its platelet receptors, what would otherwise stimulate platelet aggregation and give rise to fibrin that is responsible for thrombi formation (Rutherfurd & Gill, 2000).…”

Caseins as source of bioactive peptides

“…The sequence of amino acids in f106-171 of sheep's k-casein, known as k-caseinoglycopeptide, was shown to decrease thrombin-and collageninduced platelet aggregation in a dose-dependent manner (Qian, Joll" es, Migliore-Samour, Schoentgen, & Fiat, 1995). In the plasma of 5-day-old new borns, after breast feeding or ingestion of cow's milk-based formulae, antithrombotic peptides were detected, which had been derived from human and bovine k-caseinoglycopeptides, respectively (Chabance et al, 1995).…”

Bioactive Peptides Derived from Casein and Whey Proteins

“…The two smaller tryptic peptides ( κ -casein f106 -112 and f113 -116) exerted a minimal effect on platelet aggregation and did not inhibit fi brinogen binding. Several κ -caseinderived glycopeptides from different origins, including human and sheep, have also been shown to inhibit platelet aggregation (Qian et al 1995a(Qian et al , 1995b. The C -terminal part (residues 106 -171) of sheep κ -casein inhibits thrombin -and collageninduced platelet aggregation in a dose -dependent manner.…”

“…The C -terminal part (residues 106 -171) of sheep κ -casein inhibits thrombin -and collageninduced platelet aggregation in a dose -dependent manner. Three peptides, Lys -Asp -Gln -Asp -Lys (residues 112 -116), Thr -Ala -Gln -Val -Thr -SerThr -Glu -Val (residues 163 -171), and Gln -ValThr -Ser -Thr -Glu -Val (residues 165 -171), obtained from an enzymatic hydrolysate of GMP, completely inhibited thrombin -induced platelet aggregation (Qian et al 1995b ). Moreover, a peptide derived from a pepsin digest of sheep and human lactoferrin was shown to inhibit thrombin -induced platelet aggregation (Qian et al 1995a ).…”