Shelf life extension of strawberry juice by equivalent ultrasound, high pressure, and pulsed electric fields processes

Yıldız, Semanur; Pokhrel, Prashant Raj; Ünlütürk, Sevcan; Barbosa‐Cánovas, Gustavo V.

doi:10.1016/j.foodres.2020.110040

Cited by 68 publications

(28 citation statements)

References 64 publications

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“…10 The lethal electroporation threshold was found to be between 10 ~ 35 kV/cm. 16 Typically, in order to achieve the strong enough electric field, the EFT processes will require high applied voltages (e.g., ~ 23 kV to achieve 35 kV/cm on the electrodes with 0.65 cm distance), 17 which leads to safety issues, side reactions, and high energy consumption.…”

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confidence: 99%

Operando Investigation of Locally Enhanced Electric Field Treatment (LEEFT) Harnessing Lightning-Rod Effect for Rapid Bacteria Inactivation

2021

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The growth of undesired bacteria causes numerous problems. Here, we show that locally enhanced electric field treatment (LEEFT) can cause rapid bacteria inactivation by electroporation without any side reactions. The bacteria inactivation is studied in situ at the single-cell level on a lab-on-a-chip that has nanowedge-decorated electrodes. Rapid bacteria inactivation occurs specifically at nanowedge tips where the electric field is enhanced due to the lightning-rod effect. The mechanism study shows that the bacteria inactivation is caused by electroporation induced by the locally enhanced electric field. The bacteria inactivation performance depends on the strength of the enhanced electric field instead of the applied voltage, and no ROS generation is detected when >90% bacteria inactivation is achieved. Quick membrane pore closure under moderate LEEFT indicates that electroporation is the predominant mechanism. LEEFT only requires facile treatment to achieve bacteria inactivation, which is safe for treating delicate samples and energyefficient for large scale applications. The findings in this work can provide strong supports for the future applications of LEEFT.

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confidence: 99%

Operando Investigation of Locally Enhanced Electric Field Treatment (LEEFT) Harnessing Lightning-Rod Effect for Rapid Bacteria Inactivation

2021

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“…Traore et al [40] reported that US processing of cabbage significantly inactivated E. coli and Salmonella cells. The authors correlated the loss of bacterial membrane integrity to the loss of the metabolic potentiality during storage, reaching a maximum count of 6.1 an Y&M after 30 days of cold storage, respectively (Figu were observed, possibly because they contained epip mum acceptable microbial limits regarding TMAC an log and 3-log CFU/mL, respectively [8]. Thermal PAST crobial counts throughout the storage period (30 day counts below 1-log CFU/mL.…”

Section: Microbial Stability During Cold Storagementioning

confidence: 95%

“…The highest TMAM counts were observed, possibly because they contained epiphytic microorganisms. The maximum acceptable microbial limits regarding TMAC and Y&M count in fruit juices are 4-log and 3-log CFU/mL, respectively [8]. Thermal PAST (90 • C for 1 min) ensured low microbial counts throughout the storage period (30 days), keeping the TMAM and Y&M counts below 1-log CFU/mL.…”

Section: Microbial Stability During Cold Storagementioning

confidence: 96%

“…Combining US with other innovative techniques can reduce equipment size, provide a faster response to process control and start-up, increase production, and eliminate processing steps [4]. Some studies have reported the combined processing of US with other non-thermal technologies, such as plasma, pulsed electric fields, high pressure, and OZ [5][6][7][8][9].…”

Section: Introductionmentioning

confidence: 99%

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Ultrasound and Ozone Processing of Cashew Apple Juice: Effects of Single and Combined Processing on the Juice Quality and Microbial Stability

et al. 2021

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Standalone and sequential ultrasound (US) and ozone (OZ) processes were applied to cashew apple juice. An unsupervised method, by principal component analysis (PCA), was used to understand the effect of the non-thermal treatments on the cashew apple composition. The US processing (373 W/cm2; 10 min; 40 °C) promoted the highest peroxidase inactivation and increased the flavonoid content and antioxidant activity (DPPH ABTS and FRAP methods). The ozone processing (0.24 mg O3/mL) increased total phenolic compounds (TPC). Sequential processing was carried out by applying both of the processes, using the best processing conditions for US and OZ. Sequential processing resulted in the higher retention of yellow flavonoids than in the control and single processing. However, the effect of sequential US and OZ processing can be deleterious to vitamin C and TPC after 30 days of cold storage while maintaining the flavonoids of the cashew apple juice. Furthermore, the synergy between US and OZ reduced native microbiota counts, suggesting a microbiologically safe juice. Further studies on the mechanism of bioactive compound degradation might be helpful for product quality modulation.

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confidence: 99%

Nano-Enhanced Electric-Field Treatment Harnessing Lightning-Rod Effect for Rapid Bacteria Inactivation

Wang¹,

Brown²,

Xie³

2021

Preprint

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The growth of undesired bacteria can cause numerous problems. Seeking effective and sustainable bacteria inactivation approaches is an everlasting effort. Here, we show that nano-enhanced electric field treatment (NEEFT) can cause rapid bacteria inactivation with a lower applied voltage than bulk EFT. A lab-on-a-chip with nanowedge-modified electrodes is developed, and the bacteria inactivation in NEEFT is visualized and studied in real-time at a single-cell level. Rapid bacteria inactivation (~ 1 ms) occurs specifically at nanowedge tips where the electric field is enhanced due to the lightning-rod effect. Nanowedges with a high aspect ratio are critical for bacteria inactivation. NEEFT works for both immobilized and free-moving cells, where the free-moving cells will be first attracted to the nanowedge tips followed by rapid inactivation. The mechanism study shows that the bacteria inactivation is caused by electroporation induced by the nano-enhanced electric field. The bacteria inactivation performance depends on the strength of the enhanced electric field instead of the applied voltage. Quick pore closure and membrane recovery under moderate NEEFT indicate that electroporation is the predominant mechanism. NEEFT only requires facile treatment to achieve bacteria inactivation, which is safe for treating delicate samples and energy-efficient for large scale applications. It is also expected to find applications for targeted cell inactivation.

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Shelf life extension of strawberry juice by equivalent ultrasound, high pressure, and pulsed electric fields processes

Cited by 68 publications

References 64 publications

Operando Investigation of Locally Enhanced Electric Field Treatment (LEEFT) Harnessing Lightning-Rod Effect for Rapid Bacteria Inactivation

Operando Investigation of Locally Enhanced Electric Field Treatment (LEEFT) Harnessing Lightning-Rod Effect for Rapid Bacteria Inactivation

Ultrasound and Ozone Processing of Cashew Apple Juice: Effects of Single and Combined Processing on the Juice Quality and Microbial Stability

Nano-Enhanced Electric-Field Treatment Harnessing Lightning-Rod Effect for Rapid Bacteria Inactivation

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