'Shelf life' of &lt;I&gt;Trichomonas vaginalis&lt;/I&gt;

Kingston, Margaret; Bansal, Devanshu; Carlin, E M

doi:10.1258/095646203321043228

Cited by 63 publications

(30 citation statements)

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“…In some settings, the wet mount is transported to the microbiology laboratory and read by technicians after a significant time delay. The sensitivity of wet-mount microscopy for detecting T. vaginalis declines substantially with even relatively short time intervals between collection and examination (8). Where wet mount is available, the appropriate T. vaginalis test for wet-mount-negative subjects has not been delineated.…”

Section: Discussionmentioning

confidence: 99%

Use of an Immunochromatographic Assay for Rapid Detection of Trichomonas vaginalis in Vaginal Specimens

et al. 2005

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Trichomonas vaginalis infection is estimated to be the most widely prevalent nonviral sexually transmitted infection in the world. Wet-mount microscopy is the most common diagnostic method, although it is less sensitive than culture. The OSOM Trichomonas Rapid Test (Genzyme Diagnostics, Cambridge, Mass.) (referred to here as OSOM) is a new point-of-care diagnostic assay for T. vaginalis that uses an immunochromatographic capillary flow (dipstick) assay and provides results in 10 min. The purpose of this study was to determine the test characteristics of OSOM compared to those of a composite reference standard (CRS) comprised of wet-mount microscopy and T. vaginalis culture. This multicenter cross-sectional study enrolled sexually active women >18 years of age who presented with symptoms of vaginitis, exposure to T. vaginalis, or multiple sexual partners. Vaginal-swab specimens were obtained for T. vaginalis culture, wet mount, and rapid testing. The prevalence of T. vaginalis in this sample was 23.4% (105 of 449) by the CRS. The sensitivity and specificity of OSOM vaginal-swab specimens were 83.3 and 98.8%, respectively, while wet mount had a sensitivity and specificity of 71.4 and 100%, respectively, compared to the CRS. OSOM performed significantly better than wet mount (P ‫؍‬ 0.004) and detected T. vaginalis in samples that required 48 to 72 h of incubation prior to becoming culture positive. The performance of the rapid test was not affected by the presence of coinfections with chlamydia and gonorrhea. The OSOM Trichomonas Rapid Test is a simple, objective test that can be expected to improve the diagnosis of T. vaginalis, especially where microscopy and culture are unavailable.

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Section: Discussionmentioning

confidence: 99%

Use of an Immunochromatographic Assay for Rapid Detection of Trichomonas vaginalis in Vaginal Specimens

et al. 2005

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“…Microscopy is unlikely to detect low level infections, in which the organism load in the sample may be under 10 4 cells/ml, and therefore will potentially not be included in the fields examined on the slide (Garber, 2005). The sensitivity of this method decreases rapidly if delays are present between sample acquisition and examination, with a reduction in sensitivity to 20% caused by a as little as a 10 minute delay reported (Kingston et al, 2003). Due to this it has been suggested that any diagnostic service which cannot guarantee the ability to test samples within an hour of acquisition should use alternative methods (Stoner et al, 2013).…”

Section: Diagnosis Female Patientsmentioning

confidence: 99%

Trichomonas vaginalis: Clinical relevance, pathogenicity and diagnosis

Edwards

Burke

Smalley

et al. 2014

Critical Reviews in Microbiology

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Trichomonas vaginalis is the etiological agent of trichomoniasis, the most prevalent non-viral sexually transmitted disease worldwide. Trichomoniasis is a widespread, global health concern, and occurring at an increasing rate. Infections of the female genital tract can cause a range of symptoms, including vaginitis and cervicitis, whilst infections in males are generally asymptomatic. The relatively mild symptoms, and lack of evidence for any serious sequelae, have historically led to this disease being under diagnosed, and under researched. However, growing evidence that T. vaginalis infection is associated with disease states with high morbidity in both men and women has increased the efforts to diagnose and treat patients harbouring this parasite. The pathology of trichomoniasis results from damage to the host epithelia, caused by a variety of processes during infection, and recent work has highlighted the complex interactions between the parasite and host, commensal microbiome, and accompanying symbionts. The commercial release of a number of nucleic acid amplification tests (NAATs) has added to the available diagnostic options. Immunoassay based Point of Care testing is currently available, and a recent initial evaluation of a NAAT Point of Care system has given promising results, which would enable testing and treatment in a single visit.

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“…The wet preparation should be read within 10 min of collection, as the trichomonads will quickly loose motility and be more difficult to identify. 25 The slide should be scanned, firstly at low magnification (100Â), and then at a higher magnification (400Â) to confirm the morphology of any trichomonads and to visualise the flagella. Microscopy as a diagnostic aid for TV has the advantage that it can be performed near to the patient and in a clinic setting.…”

Section: Laboratory Investigationsmentioning

confidence: 99%