Shell-Less Chick Embryo Culture as an Alternative in vitro Model to Investigate Glucose-Induced Malformations in Mammalian Embryos

Datar, Savita; Bhonde, Ramesh

doi:10.1900/rds.2005.2.221

Cited by 35 publications

(30 citation statements)

References 38 publications

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“…Hence, it is unlikely that caudal regression was mediated by vascular disruption at least in the present chick model. Caudal regression syndrome-like anomalies has been reported to develop in glucose-treated and insulin-treated chick embryos (33,34). In both studies, the mortality and morbidity were developmental stage dependent.…”

Section: Discussionmentioning

confidence: 92%

Caudal Regression and Tracheoesophageal Malformation Induced by Adriamycin: A Novel Chick Model of VATER Association

et al. 2009

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VATER association represents a cluster of Vertebral, Anal, Tracheo-Esophageal, Radial and Renal malformations, and caudal regression syndrome is an entity consisting of a spectrum of congenital anomalies of lower spine and hips associated with genitourinary and lower limb defects. The concurrence of various malformations may be explained by a common defect in blastogenesis, but direct evidence is yet to be accumulated. Here, by the use of autofluorescence and the teratogenic effect of adriamycin, we demonstrated that adriamycin administered to eggs of White Leghorns distributes to the caudal portion of the embryo and foregut epithelium and induces caudal regression and tracheal and pulmonary agenesis. The induction of caudal regression syndrome-like anomaly was developmental stage and dose dependent. Embryos with caudal regression demonstrated tracheoesophageal anomalies, one of the defects included in VATER association. The stages at which anomalies were produced corresponded to that of human embryos between days 22 and 26 (Carnegie stages 10 -11). In view of the antitumor activity of adriamycin by intercalating to double-stranded DNA of undifferentiated cells undergoing rapid cell division, it is possible that adriamycin had preferentially attacked cells in the caudal end where pronounced proliferation takes place during this narrow period of greatest susceptibility. (Pediatr Res 65: 607-612, 2009)

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Section: Discussionmentioning

confidence: 92%

Caudal Regression and Tracheoesophageal Malformation Induced by Adriamycin: A Novel Chick Model of VATER Association

et al. 2009

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“…Work from our lab has shown that isolated chick islets are suitable for screening of hypoglycemic agents and for islet banking (Figure 1) [12,13]. Our work has also revealed that the shell-less chick embryo culture system can be used for studying glucose-induced malformations similar to those observed in mammalian embryos [14].…”

Section: Introductionmentioning

confidence: 80%

“…Different diabetic malformations of mammalian gestational diabetes, such as embryonic dismorphogenesis, rotational defects, cardiovascular abnormalities, macrosomia, macrocephaly, and retarded growth could be observed using this system. This study indicated that the developing chick embryo provides an excellent alternative model system to mammalian embryo models for the study of glucose-induced malformations [14]. The shell-less chick embryo culture system can also be employed in biomedical research for exploring the effects of angiogenic agents, vitamins, antibiotics, viruses, and carcinogenic agents.…”

Section: Chick Embryo As a Model For Depicting Glucose-induced Malformentioning

confidence: 95%

“…We have developed a simple shell-less chick embryo culture system [14], which supports the culturing of chick embryos from the very early stages of morphogenetic movements through the stages of active organogenesis to the seventh day of incubation, outside the eggshell in a glass bowl. This culture system is economical and highly reproducible.…”

Section: Chick Embryo As a Model For Depicting Glucose-induced Malformentioning

confidence: 99%

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Modeling Chick to Assess Diabetes Pathogenesis and Treatment

Datar¹,

Bhonde²

2011

Rev Diabet Stud

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■ AbstractAnimal models have been used extensively in diabetes research. Studies on animal models have contributed to the discovery and purification of insulin, development of new therapeutic approaches, and progress in fundamental and clinical research. However, conventional rodent and large animal mammalian models face ethical, practical, or technical limitations. Therefore, it would be beneficial developing an alternative model for diabetes research which would overcome these limitations. Amongst other vertebrates, birds are phylogenically closer to mammals, and amongst birds, the chick has been used as one of the favored models in developmental biology, toxicology, cancer research, immunology, and drug testing. Chicken eggs are readily available, have a short incubation period and easily accessible embryos. Based on these inimitable advantages, the present review article aims to discuss the suitability of the chick as a model system to study specific aspects of diabetes. The review focuses on the application of i) chick pancreatic islets for screening of antidiabetic agents and for islet banking, (ii) shell-less chick embryo culture as a model to study hyperglycemia-induced malformations observed in mammalian embryos, and (iii) chick chorioallantoic membrane (CAM) to examine glucose-induced endothelial damage leading to inhibition of angiogenesis.

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“…enabled study of organogenesis, angiogenesis, hematology, and the physiological function of the cardiovascular system in bird embryos just a few days old (Burggren et al, 2004;Datar and Bhonde, 2005;Khorrami et al, 2008;Branum et al, 2013). Figure 6 illustrates the process of growing embryos in shell-less culture as well as physiological information that can be derived from them.…”

Section: Organic Toxicants As Developmental Disruptorsmentioning

confidence: 99%

Cardio-respiratory development in bird embryos: new insights from a venerable animal model

2016

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-The avian embryo is a time-honored animal model for understanding vertebrate development. A key area of extensive study using bird embryos centers on developmental phenotypic plasticity of the cardio-respiratory system and how its normal development can be affected by abiotic factors such as temperature and oxygen availability. Through the investigation of the plasticity of development, we gain a better understanding of both the regulation of the developmental process and the embryo's capacity for self-repair. Additionally, experiments with abiotic and biotic stressors during development have helped delineate not just critical windows for avian cardio-respiratory development, but the general characteristics (e.g., timing and dose-dependence) of critical windows in all developing vertebrates. Avian embryos are useful in exploring fetal programming, in which early developmental experiences have implications (usually negative) later in life. The ability to experimentally manipulate the avian embryo without the interference of maternal behavior or physiology makes it particularly useful in future studies of fetal programming. The bird embryo is also a key participant in studies of transgenerational epigenetics, whether by egg provisioning or effects on the germline that are transmitted to the F1 generation (or beyond). Finally, the avian embryo is heavily exploited in toxicology, in which both toxicological testing of potential consumer products as well as the consequences of exposure to anthropogenic pollutants are routinely carried out in the avian embryo. The avian embryo thus proves useful on numerous experimental fronts as an animal model that is concurrently both of adequate complexity and sufficient simplicity for probing vertebrate cardio-respiratory development.

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Shell-Less Chick Embryo Culture as an Alternative in vitro Model to Investigate Glucose-Induced Malformations in Mammalian Embryos

Cited by 35 publications

References 38 publications

Caudal Regression and Tracheoesophageal Malformation Induced by Adriamycin: A Novel Chick Model of VATER Association

Caudal Regression and Tracheoesophageal Malformation Induced by Adriamycin: A Novel Chick Model of VATER Association

Modeling Chick to Assess Diabetes Pathogenesis and Treatment

Cardio-respiratory development in bird embryos: new insights from a venerable animal model

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