Shewanella algae: First case report of the fast emerging marine pathogen from squamous cell carcinoma patient in India

Sumathi, B; Kumarswamy, S.V.; Amritam, Usha; Arjunan, Ravi

doi:10.4103/2278-330x.136819

Cited by 10 publications

(7 citation statements)

References 3 publications

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“…Furthermore, it has been verified that Shewanella species, as marine pathogens, are associated with human diseases (12). Misidentifications to the species level were fairly common in clinical diagnoses due to the poor discernment system (39). In this study, 86 Shewanella strains collected from environmental, food, and clinical samples in China were mainly defined as S. algae, followed by S. xiamenensis, S. chilikensis, S. indica, S. seohaensis, and S. carassii, via the MLSA scheme.…”

Section: Discussionmentioning

confidence: 99%

Multilocus Sequence Analysis, a Rapid and Accurate Tool for Taxonomic Classification, Evolutionary Relationship Determination, and Population Biology Studies of the Genus Shewanella

Fang

Wang²,

Liu³

et al. 2019

Appl Environ Microbiol

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The genus Shewanella comprises a group of marine-dwelling species with worldwide distribution. Several species are regarded as causative agents of food spoilage and opportunistic pathogens of human diseases. In this study, a standard multilocus sequence analysis (MLSA) based on six protein-coding genes (gyrA, gyrB, infB, recN, rpoA, and topA) was established as a rapid and accurate identification tool in 59 Shewanella type strains. This method yielded sufficient resolving power in regard to enough informative sites, adequate sequence divergences, and distinct interspecies branches. The stability of phylogenetic topology was supported by high bootstrap values and concordance with different methods. The reliability of the MLSA scheme was further validated by identical phylogenies and high correlations of genomes. The MLSA approach provided a robust system to exhibit evolutionary relationships in the Shewanella genus. The split network tree proposed twelve distinct monophyletic clades with identical GϩC contents and high genetic similarities. A total of 86 tested strains were investigated to explore the population biology of the Shewanella genus in China. The most prevalent Shewanella species was Shewanella algae, followed by Shewanella xiamenensis, Shewanella chilikensis, Shewanella indica, Shewanella seohaensis, and Shewanella carassii. The strains frequently isolated from clinical and food samples highlighted the importance of increasing the surveillance of Shewanella species. Based on the combined genetic, genomic, and phenotypic analyses, Shewanella upenei should be considered a synonym of S. algae, and Shewanella pacifica should be reclassified as a synonym of Shewanella japonica. IMPORTANCE The MLSA scheme based on six housekeeping genes (HKGs) (gyrA, gyrB, infB, recN, rpoA, and topA) is well established as a reliable tool for taxonomic, evolutionary, and population diversity analyses of the genus Shewanella in this study. The standard MLSA method allows researchers to make rapid, economical, and precise identification of Shewanella strains. The robust phylogenetic network of MLSA provides profound insight into the evolutionary structure of the genus Shewanella. The population genetics of Shewanella species determined by the MLSA approach plays a pivotal role in clinical diagnosis and routine monitoring. Further stud-2019. Multilocus sequence analysis, a rapid and accurate tool for taxonomic classification, evolutionary relationship determination, and population biology studies of the genus Shewanella. Appl Environ Microbiol 85:e03126-18. https://doi. Downloaded fromies on remaining species and genomic analysis will enhance a more comprehensive understanding of the microbial systematics, phylogenetic relationships, and ecological status of the genus Shewanella.

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Section: Discussionmentioning

confidence: 99%

Multilocus Sequence Analysis, a Rapid and Accurate Tool for Taxonomic Classification, Evolutionary Relationship Determination, and Population Biology Studies of the Genus Shewanella

Fang

Wang²,

Liu³

et al. 2019

Appl Environ Microbiol

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“…Furthermore, it has been verified that Shewanella species, as marine pathogens, are associated with human diseases (12). Misidentifications to the species level were fairly common in clinical diagnoses due to the poor discernment system (45). In this study, eighty-six Shewanella strains collected from the environment, food and clinical samples in China were mainly defined as S. algae , followed by S. xiamenensis, S. chilikensis, S. indica, S. seohaensis , and S. carassii via the MLSA scheme.…”

Section: Discussionmentioning

confidence: 99%

Multilocus sequence analysis, a rapid and accurate identification tool for taxonomic classification, evolutionary relationship and population biology of the genus Shewanella

Fang

Wang

Liu

et al. 2019

Preprint

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22The genus Shewanella comprises a group of marine-dwelling species with worldwide 23 distribution. Several species are regarded as causative agents of food spoilage and 24 opportunistic pathogens of human diseases. In this study, a standard multilocus 25 sequence analysis (MLSA) based on six protein-coding genes (gyrA, gyrB, infB, recN, 26 rpoA and topA) was established as a rapid and accurate identification tool in fifty-nine 27 The nucleotide sequences of six HKGs are deposited in GenBank nucleotide sequence database under accession numbers of gyrA: MH090144-MH090185; gyrB: MH090186-MH090202; infB: MH090203-MH090244; recN: MH090245-MH090286; rpoA: MH090287-MH090328; and topA: MH090329-MH090370. 2 type Shewanella strains. This method yielded sufficient resolving power in regard to 28 enough informative sites, adequate sequence divergences and distinct interspecies 29 branches. The stability of phylogenetic topology was supported by high bootstrap 30 values and concordance with different methods. The reliability of the MLSA scheme 31 was further validated by identical phylogenies and high correlations of genomes. The 32 MLSA approach provided a robust system to exhibit evolutionary relationships in the 33 Shewanella genus. The split network tree proposed twelve distinct monophyletic 34 clades with identical G+C contents and high genetic similarities. Eighty-six tested 35 strains were investigated to explore the population biology of the Shewanella genus in 36 China. The most prevalent Shewanella species were Shewanella algae, Shewanella 37 xiamenensis, Shewanella chilikensis, Shewanella indica, Shewanella seohaensis and 38 Shewanella carassii. The strains frequently isolated from clinical and food samples 39 highlighted the importance of increasing the surveillance of Shewanella species. 40 Combined with the genetic, genomic and phenotypic analyses, Shewanella upenei 41 should be considered a synonym of S. algae, and Shewanella pacifica should be 42 reclassified as a synonym of Shewanella japonica. 43 IMPORTANCE 44 The MLSA scheme based on six HKGs (gyrA, gyrB, infB, recN, rpoA and topA) is 45 well established as a reliable tool for taxonomic, evolutionary and epidemiological 46 analyses of the genus Shewanella in this study. The standard MLSA method allows 47 48 The robust phylogenetic network of MLSA provides profound insight into the 49 evolutionary structure of the genus Shewanella. The population genetics of 50 Shewanella species determined by the MLSA approach plays a pivotal role in clinical 51 diagnosis and routine monitoring. Further studies on remaining species and genomic 52 analysis will enhance a more comprehensive understanding of the microbial 53 systematics, phylogenetic relationships and ecological status of the genus Shewanella.54

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“…Reports of infection with S. algae species in human cases are increasing, especially during the summer months and in tropical areas, such as India, China, and Taiwan [ 11 – 14 ]. In general, S. algae can be considered an opportunistic pathogen in humans exposed to a marine environment when it infects people via an existing soft tissue ulcer [ 15 – 22 ].…”

Section: Introductionmentioning

confidence: 99%

The Pathogenicity ofShewanella algaeand Ability to Tolerate a Wide Range of Temperatures and Salinities

Tseng

Liu

Lee

et al. 2018

Canadian Journal of Infectious Diseases and Medical Microbiolog

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Shewanella algae is a rod-shaped Gram-negative marine bacterium frequently found in nonhuman sources such as aquatic ecosystems and has been shown to be the pathogenic agent in various clinical cases due to the ingestion of raw seafood. The results of this study showed that S. algae was present in approximately one in four samples, including water and shellfish samples. Positive reactions (API systems) in S. algae strains were seen for gelatinase (gelatin); however, negative reactions were found for indole production (tryptophan). S. algae is adapted to a wide range of temperatures (4°C, 25°C, 37°C, and 42°C) and salinity. Temperature is a key parameter in the pathogenicity of S. algae as it appears to induce hemolysis at 25°C and 37°C. S. algae exhibits pathogenic characteristics at widely varying temperatures, which suggests that it may have the ability to adapt to climate change.

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Shewanella algae: First case report of the fast emerging marine pathogen from squamous cell carcinoma patient in India

Cited by 10 publications

References 3 publications

Multilocus Sequence Analysis, a Rapid and Accurate Tool for Taxonomic Classification, Evolutionary Relationship Determination, and Population Biology Studies of the Genus Shewanella

Multilocus Sequence Analysis, a Rapid and Accurate Tool for Taxonomic Classification, Evolutionary Relationship Determination, and Population Biology Studies of the Genus Shewanella

Multilocus sequence analysis, a rapid and accurate identification tool for taxonomic classification, evolutionary relationship and population biology of the genus Shewanella

The Pathogenicity ofShewanella algaeand Ability to Tolerate a Wide Range of Temperatures and Salinities

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