Shewanella baltica strain JD0705 isolated from the mangrove wetland soils in Thailand and characterization of its ligninolytic performance

Abstract

Abstract. Chantarasiri A. 2021. Shewanella baltica strain JD0705 isolated from the mangrove wetland soils in Thailand and characterization of its ligninolytic performance. Biodiversitas 22: 354-361. Lignin is a complex biopolymer and the third component by mass of lignocellulosic plant biomass. Its recalcitrant property hampers the hydrolysis and utilization of cellulose and hemicellulose in the lignocellulosic biomass. Thus, the delignification of lignocellulosic biomass is an enormous challenge for emerging bio-based applications. This study presented a potential ligninolytic bacterium for potential use in the biological delignification process under mild conditions. This bacterium was isolated from the mangrove wetland soils in Thailand, characterized and identified as psychrotrophic Shewanella baltica strain JD0705. It was determined for ligninolytic activity and showed laccase activity at 5.23 ± 0.10 U/mL. The optimum temperature and pH for the laccase activity were observed to be 25°C at a pH of 7.0 respectively with a stability range of 20-30°C temperature and pH of 7.0-8.0. S. baltica strain JD0705 was used in the biological delignification of rice husk powder and promoted the hydrolysis of rice husk powder to obtain more liberating sugar content. The findings from this study indicated that feasibility of using this ligninolytic bacterium for the production of laccase and the delignification of lignocellulosic plant biomass.

Diversity and activity of amylase-producing bacteria isolated from mangrove soil in Thailand

“…It is mostly overgrown by halophilic plants, including Avicennia alba Blume., Bruguiera gymnorrhiza (L.) Lamk., and Rhizophora apiculata Blume (Chantarasiri 2021a). This area was demonstrated as the identical area in the previous studies of Chantarasiri (2015;2021a;2021b) which reported the isolation, genetic identification, and enzymatic characterization of many cellulolytic, phenanthrenedegrading, and ligninolytic bacteria, respectively. Therefore, this mangrove forest was considered as a biodiversity hotspot for a bacterial isolation study in the east region of Thailand.…”

“…Thirty mangrove soil samples were collected from the Phra Chedi Klang Nam Mangrove Forest. The texture collected soil samples was sandy and muddy clays according to the previous study of Chantarasiri (2021b). The temperature measured in situ by a needle probe thermometer ranged from 28.9°C to 32.0°C, and the average temperature was 30.01  1.61°C.…”