Shh regulates chick Ebf1 gene expression in somite development

El-Magd, Mohammed A.; Allen, Steve; McGonnell, Imelda M.; Mansour, Ali; Otto, Anthony; Patel, Ketan

doi:10.1016/j.gene.2014.10.028

Cited by 17 publications

(55 citation statements)

References 68 publications

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“…It has also been shown that Ebf genes are expressed in the pharyngeal arches of the developing chick in the same domain as Tbx1 (El-Magd et al, 2014), indicating that a role for Ebf-Tbx1 interactions in the pharyngeal mesoderm might be conserved from tunicates to vertebrates. Ebf2 and Ebf3 are also expressed in the somites of chicken embryos, where they might be involved in skeletal development (El-Magd et al, 2013). In Amphioxus, the single homolog of Tbx1 is expressed in the developing branchial arches (Mahadevan et al, 2004), and although neither Ebf nor MyoD expression have been reported in branchial arches of Amphioxus, Tbx1, Ebf and MyoD homologues are all expressed in somitic mesoderm (Mahadevan et al, 2004;Mazet et al, 2004;Schubert et al, 2003;Urano et al, 2003).…”

Section: Discussionmentioning

confidence: 99%

Rewiring of an ancestral Tbx1/10-Ebf-Mrf network for pharyngeal muscle specification in distinct embryonic lineages

Tolkin

Christiaen

2016

Development

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Skeletal muscles arise from diverse embryonic origins in vertebrates, yet converge on extensively shared regulatory programs that require muscle regulatory factor (MRF)-family genes. Myogenesis in the tail of the simple chordate Ciona exhibits a similar reliance on its single MRF-family gene, and diverse mechanisms activate Ci-Mrf. Here, we show that myogenesis in the atrial siphon muscles (ASMs) and oral siphon muscles (OSMs), which control the exhalant and inhalant siphons, respectively, also requires Mrf. We characterize the ontogeny of OSM progenitors and compare the molecular basis of Mrf activation in OSM versus ASM. In both muscle types, Ebf and Tbx1/10 are expressed and function upstream of Mrf. However, we demonstrate that regulatory relationships between Tbx1/10, Ebf and Mrf differ between the OSM and ASM lineages. We propose that Tbx1, Ebf and Mrf homologs form an ancient conserved regulatory state for pharyngeal muscle specification, whereas their regulatory relationships might be more evolutionarily variable.

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Section: Discussionmentioning

confidence: 99%

Rewiring of an ancestral Tbx1/10-Ebf-Mrf network for pharyngeal muscle specification in distinct embryonic lineages

Tolkin

Christiaen

2016

Development

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“…To date, 4 (m Ebf1-4 ) and 3 EBF members (c Ebf1-3 ) have been defined in the mouse and the chick, respectively [Garel et al, 1997;Nieminen et al, 2000;Wang et al, 2002;Mella et al, 2004]. Several previous studies have detailed the expression profile of Ebf genes in the lymphatic, adipose, central nervous, muscular and skeletal tissues as well as in feather buds [reviewed by Dubois and Vincent, 2001;Liberg et al, 2002;El-Magd, 2011;El-Magd et al, 2013, 2014b. The pharyngeal arches (PAs) are temporary embryonic structures that develop bilaterally as a series of five (PA I, II, III, IV and VI) symmetrical bulges on the sides of the vertebrate head.…”

Section: Introductionmentioning

confidence: 99%

“…Previous studies have shown that Bmp 2 is likely the Bmp signal required for the production of migratory cranial NCCs, as its expression pattern is consistent with migration and its mutation produces underdeveloped branchial arches that lack detectable migratory cranial NCCs [Kanzler et al, 2000;Ohnemus et al, 2002]. Recently, we described the expression patterns of cEbf genes in somites and feather buds, and found that the expression is regulated by both Bmp and Sonic hedgehog (Shh) [El-Magd, 2011;El-Magd et al, 2013, 2014b. Furthermore, these two signaling molecules are involved in the development and patterning of the NT, NC and placodes [Kanzler et al, 2000;Liem et al, 2000;Ohnemus et al, 2002].…”

Section: Introductionmentioning

confidence: 99%

Regulation of Chick <b><i>Ebf1-3 </i></b>Gene Expression in the Pharyngeal Arches, Cranial Sensory Ganglia and Placodes

El-Magd

Saleh²,

Farrag³

et al. 2014

Cells Tissues Organs

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This study was conducted to identify the regulation of the expression of the cEbf1-3 (chick early B-cell factor 1-3) genes in the pharyngeal arches (PAs), cranial sensory ganglia and placodes. cEbf1 and cEbf3 were mainly expressed in the cranial neural crest cells (NCCs) occupying the PAs, but cEbf2 was expressed in the mesenchymal core. cEbf1-3 were prominently expressed in the olfactory placodes, but cEbf1 and cEbf3 were only expressed in the otic vesicle. cEbf1 was expressed in all cranial sensory ganglia, cEbf2 (only) in the dorsolateral ganglia and cEbf3 in the trigeminal and vestibular ganglia. The removal of the source (the cranial neural tube) of the cranial NCCs before their migration to the PAs led to downregulation of cEbf1 and cEbf3 and upregulation of cEbf2 expression. Gain- and loss-of-function experiments showed that sonic hedgehog did not regulate cEbf1-3 expression in the PAs or associated ganglia. Bone morphogenetic protein 2 (Bmp2) can, however, directly and indirectly regulate cEbf1 and cEbf3 expression in the PAs and the proximal (NCC-derived) portion, but not the distal (placodal-derived) portion of the cranial sensory ganglia. Conversely, cEbf2 expression was upregulated following injection of Noggin before the migration of NCCs, but did not change after the overexpression of either Noggin or Bmp2 in the arch after NCC migration. In conclusion, Bmp2 regulates cEbf1 and cEbf3 expression in PAs and cranial sensory ganglia both directly and indirectly, via the migration of cranial NCCs. However, cEbf2 expression in the mesenchymal core of PAs is controlled by other undetermined signals.

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“…Although it belongs to the Ebf family of proteins, Ebf2 is not expressed in mature B cells; rather, it is abundantly expressed in adipocytes, neurons, and immature osteoblasts (7)(8)(9). Ebf2 has been shown to play a role in establishing the osteoblast niche for hematopoiesis (10), regulating somite development (11), and initiating white adipocyte differentiation (12). Recently, the same group of authors discovered that Ebf2 cooperates with peroxisome proliferator-activated recep=tor-γ (PPARγ) to regulate the expression of brown adipocyte-selective genes in classical brown adipocytes (13).…”

mentioning

confidence: 99%

“…It will be important to also assess functional differences in these cell types, such as differences in metabolism. Several different pathways regulate Ebf family gene expression and transcriptional activity, including Notch (14), Hedgehog (15), and bone morphogenetic protein (BMP) signaling (11). Hedgehog signaling in adipose stromal vascular cells primes these precursors for osteogenic differentiation and suppresses adipogenesis (16), suggesting Hedgehog is unlikely to be upstream of Ebf2.…”

mentioning

confidence: 99%