Social and economic position and power shape everyone, including scientists and researchers. The way researchers do conservation science and the voices centered in the process are a result of researcher upbringing, experiences, access to resources, and values and are a manifestation of positionality. Positionality is a concept that can help one think about one's position and power in one's work. Creating a successful research partnership requires careful thinking about how equity, diversity, inclusivity, and accessibility are accounted for in the research environment. We drew on our own experiences as early career, mid‐career, and Indigenous researchers to explore researcher positionality and how understanding one's positionality can bring to the fore power dynamics in conservation science and research. We focused on the use of reflexive practice to recognize diverse roles and responsibilities, build strong project governance, and enrich relationships. We considered 2 large research partnerships, Apoqnmatulti'k (Mi'kmaw for we help each other) and the SakKijânginnaniattut Nunatsiavut Sivunitsangit (Inuttitut for Sustainable Nunatsiavut Futures) project, to examine moments of tension and interrogation of power and the ways in which this interrogation led to stronger relationships and better research. We advise that large transdisciplinary and cross‐cultural research teams use positionality and reflexivity to explicitly make choices about power dynamics in the context of executing partnership‐driven work. This can be accomplished through personal and collective interrogation of the power dynamics at play in project administration, research questions, and interpersonal relationships.