This Special Issue aims to reflect on knowledge co-production and transdisciplinarity, exploring the mutual interaction between water governance and water research. We do so with contributions that bring examples from diverse parts of the world: Bolivia, Canada, Germany, Ghana, Namibia, the Netherlands, Palestine, and South Africa. Key insights brought by these contributions include the importance of engaging the actors from early stages of transdisciplinary research, and the need for an in-depth understanding of the diverse needs, competences, and power of actors and the water governance system in which knowledge co-production takes place. Further, several future research directions are identified, such as the examination of knowledge backgrounds according to the individual and collective thought styles of different actors. Together, the eight papers included in this Special Issue constitute a significant step toward a better understanding of knowledge co-production and transdisciplinarity, with a common thread for being reflective and clear about their complexity, and the political implications and risks they pose for inclusive, plural and just water research and governance.