Shifts between ammonia-oxidizing bacteria and archaea in relation to nitrification potential across trophic gradients in two large Chinese lakes (Lake Taihu and Lake Chaohu)

Hou, Jie; Song, Changchun; Cao, Xiuyun; Zhou, Yiyong

doi:10.1016/j.watres.2013.01.042

Cited by 114 publications

(85 citation statements)

References 53 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Despite AOA outnumbering AOB, AOB abundance was correlated with total nitrification rates for all stations and all seasons (p < 0.005), but AOA abundance was not. This result agrees with a previous study on Taihu Lake sediments, where AOA were negatively correlated (r = 0.53, p < 0.05) with potential nitrification rates (0-3.0 µg NO − 3 N g −1 dry sediment; Hou et al, 2013). We speculate that AOA oxidized NH + 4 at lower rates due to oversaturation and inhibition and may not have contributed as much as AOB to nitrification rates in our study.…”

Section: Ammonia Oxidizer Abundancesupporting

confidence: 93%

“…In contrast, AOB, with higher K m , thrive at higher NH + 4 concentrations at nearshore locations (mean nearshore NH + 4 concentration = 31.3 µM). These results agree with previous research in Taihu Lake, where AOA outnumbered AOB in sediments at mesotrophic sites and AOB were more abundant at hypereutrophic locations (Hou et al, 2013). Another study on Taihu Lake sediments also reported that both AOA abundance and AOA : AOB were negatively correlated with ambient NH + 4 concentration (Wu et al, 2010).…”

Section: Ammonia Oxidizer Abundancesupporting

confidence: 92%

“…Another sediment study revealed that, while AOOs were present at all sites, the distribution of AOA and AOB depended on lake trophic status (Hou et al, 2013). Abundance of AOA decreased, while AOB increased, with increasing trophic status, following the substrate concentration hypothesis presented in kinetic experiments (Martens-Habbena et al, 2009).…”

Section: Introductionmentioning

confidence: 84%

“…AOA often dominate in environments with low substrate concentrations, such as the open ocean or oligotrophic lakes (Beman et al, 2008;Bollmann et al, 2014;Newell et al, 2011), while AOB are often more abundant in nutrient rich waters and soils (Hou et al, 2013;Jia and Conrad, 2009;Kowalchuk and Stephen, 2001;Verhamme et al, 2011). This substrate concentration adaptation is dictated by different physiological abilities to assimilate NH (Francis et al, 2005), some species adapt to higher substrate concentrations (Jung et al, 2011;Tourna et al, 2011).…”

Section: Ammonia Oxidizer Abundancementioning

confidence: 99%

See 3 more Smart Citations

Nitrification and ammonium dynamics in Taihu Lake, China: seasonal competition for ammonium between nitrifiers and cyanobacteria

et al. 2018

View full text Add to dashboard Cite

Abstract. Taihu Lake is hypereutrophic and experiences seasonal, cyanobacterial harmful algal blooms. These Microcystis blooms produce microcystin, a potent liver toxin, and are linked to anthropogenic nitrogen (N) and phosphorus (P) loads to lakes. Microcystis spp. cannot fix atmospheric N and must compete with ammonia-oxidizing and other organisms for ammonium (NH + 4 ). We measured NH + 4 regeneration and potential uptake rates and total nitrification using stable-isotope techniques. Nitrification studies included abundance of the functional gene for NH + 4 oxidation, amoA, for ammonia-oxidizing archaea (AOA) and bacteria (AOB). Potential NH + 4 uptake rates ranged from 0.02 to 6.80 µmol L −1 h −1 in the light and from 0.05 to 3.33 µmol L −1 h −1 in the dark, and NH + 4 regeneration rates ranged from 0.03 to 2.37 µmol L −1 h −1 . Nitrification rates exceeded previously reported rates in most freshwater systems. Total nitrification often exceeded 200 nmol L −1 d −1 and was > 1000 nmol L −1 d −1 at one station near a river discharge. AOA amoA gene copies were more abundant than AOB gene copies (p < 0.005) at all times; however, only abundance of AOB amoA (not AOA) was correlated with nitrification rates for all stations and all seasons (p < 0.005). Nitrification rates in Taihu Lake varied seasonally; at most stations, rates were highest in March, lower in June, and lowest in July, corresponding with cyanobacterial bloom progression, suggesting that nitrifiers were poor competitors for NH + 4 during the bloom.Regeneration results suggested that cyanobacteria relied extensively on regenerated NH + 4 to sustain the bloom. Internal NH + 4 regeneration exceeded external N loading to the lake by a factor of 2 but was ultimately fueled by external N loads. Our results thus support the growing literature calling for watershed N loading reductions in concert with existing management of P loads.

show abstract

Section: Ammonia Oxidizer Abundancesupporting

confidence: 93%

Section: Ammonia Oxidizer Abundancesupporting

confidence: 92%

Section: Introductionmentioning

confidence: 84%

Section: Ammonia Oxidizer Abundancementioning

confidence: 99%

See 2 more Smart Citations

Nitrification and ammonium dynamics in Taihu Lake, China: seasonal competition for ammonium between nitrifiers and cyanobacteria

et al. 2018

View full text Add to dashboard Cite

show abstract

“…Nitrification occurs in two steps: the first and rate-limiting step is the oxidation of ammonia to hydroxylamine, which is further oxidized to nitrite. The second step, performed by nitrite-oxidizing bacteria, encompasses the oxidation of nitrite to nitrate (15,16). The oxidation of ammonia is catalyzed by the enzyme ammonia monooxygenase, which is produced by a few (phylogenetically restricted) proteobacteria (ammonia-oxidizing bacteria [AOB]) and some archaea belonging to the phylum Thaumarchaeota (ammonia-oxidizing archaea [AOA]) (14,17,18).…”

mentioning

confidence: 99%

Different Effects of Transgenic Maize and Nontransgenic Maize on Nitrogen-Transforming Archaea and Bacteria in Tropical Soils

Cotta

Dias

Marriel

et al. 2014

Appl Environ Microbiol

View full text Add to dashboard Cite

dThe composition of the rhizosphere microbiome is a result of interactions between plant roots, soil, and environmental conditions. The impact of genetic variation in plant species on the composition of the root-associated microbiota remains poorly understood. This study assessed the abundances and structures of nitrogen-transforming (ammonia-oxidizing) archaea and bacteria as well as nitrogen-fixing bacteria driven by genetic modification of their maize host plants. The data show that significant changes in the abundances (revealed by quantitative PCR) of ammonia-oxidizing bacterial and archaeal communities occurred as a result of the maize host being genetically modified. In contrast, the structures of the total communities (determined by PCRdenaturing gradient gel electrophoresis) were mainly driven by factors such as soil type and season and not by plant genotype. Thus, the abundances of ammonia-oxidizing bacterial and archaeal communities but not structures of those communities were revealed to be responsive to changes in maize genotype, allowing the suggestion that community abundances should be explored as candidate bioindicators for monitoring the possible impacts of cultivation of genetically modified plants.

show abstract

Illumina sequencing‐based analysis of sediment bacteria community in different trophic status freshwater lakes

et al. 2017

View full text Add to dashboard Cite

Sediment bacterial community is the main driving force for nutrient cycling and energy transfer in aquatic ecosystem. A thorough understanding of the community's spatiotemporal variation is critical for us to understand the mechanisms of cycling and transfer. Here, we investigated the sediment bacterial community structures and their relations with environmental factors, using Lake Taihu as a model system to explore the dependence of biodiversity upon trophic level and seasonality. To combat the limitations of conventional techniques, we employed Illumina MiSeq Sequencing and LeFSe cladogram to obtain a more comprehensive view of the bacterial taxonomy and their variations of spatiotemporal distribution. The results uncovered a 1,000‐fold increase in the total amount of sequences harvested and a reverse relationship between trophic level and the bacterial diversity in most seasons of a year. A total of 65 phyla, 221 classes, 436 orders, 624 families, and 864 genera were identified in the study area. Delta‐proteobacteria and gamma‐proteobacteria prevailed in spring/summer and winter, respectively, regardless trophic conditions; meanwhile, the two classes dominated in the eutrophication and mesotrophication lake regions, respectively, but exclusively in the Fall. For LEfSe analysis, bacterial taxon that showed the strongest seasonal or spatial variation, majority had the highest abundance in spring/summer or medium eutrophication region, respectively. Pearson's correlation analysis indicated that 5 major phyla and 18 sub‐phylogenetic groups showed significant correlation with trophic status. Canonical correspondence analysis further revealed that porewater NH 4 +‐N as well as sediment TOM and NO x‐N are likely the dominant environmental factors affecting bacterial community compositions.

show abstract

Shifts between ammonia-oxidizing bacteria and archaea in relation to nitrification potential across trophic gradients in two large Chinese lakes (Lake Taihu and Lake Chaohu)

Cited by 114 publications

References 53 publications

Nitrification and ammonium dynamics in Taihu Lake, China: seasonal competition for ammonium between nitrifiers and cyanobacteria

Nitrification and ammonium dynamics in Taihu Lake, China: seasonal competition for ammonium between nitrifiers and cyanobacteria

Different Effects of Transgenic Maize and Nontransgenic Maize on Nitrogen-Transforming Archaea and Bacteria in Tropical Soils

Illumina sequencing‐based analysis of sediment bacteria community in different trophic status freshwater lakes

Contact Info

Product

Resources

About