Shifts in the Soil Microbial Community and Enzyme Activity Under Picea crassifolia Plantations and Natural Forests

Abstract

Soil microbes are crucial for regulating biogeochemical cycles and maintaining forest ecosystem sustainability; however, the understanding of microbial communities and enzyme activity under natural and plantation forests in plateau regions remains limited. Using soil samples from 15-, 30-, and 50-year-old Picea crassifolia plantations and a natural forest (NF) in eastern Qinghai, China, this study assessed physicochemical properties, microbial communities, and enzyme activity across three soil layers. Microbial composition was characterized using the phospholipid fatty acid (PLFA) method, which is sensitive to structural changes. The PLFAs of bacteria, fungi, and actinomycetes accounted for 58.31%–74.20%, 8.91%–16.83%, and 3.41%–10.41% of the total PLFAs in all forests, respectively. There were significant differences between the NF and plantations, with the NF exhibiting higher PLFA abundance and enzyme activities than plantations, except for fungal PLFAs. PLFAs in plantations increased with the plantation age. However, the fungi-to-bacteria ratio was lower in the NF than in plantations. Finally, a redundancy analysis revealed that soil properties influence microbial composition and enzyme functionality significantly. These findings highlight the influence of stand age on microbial communities and structure, offering valuable insights for forest management practices aimed at conserving natural forests.